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Microbial agent for preventing and controlling apple root rot and preparation method thereof

A technology of microbial inoculum and microbial strain is applied in the field of microbial inoculum for preventing and controlling apple root rot and its preparation field, which can solve problems such as unsatisfactory effects, achieve significant prevention and control effects, improve disease resistance, and increase yield Effect

Inactive Publication Date: 2020-04-14
SHANXI HAOZHIDA BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method has a certain effect on the treatment of apple tree root rot, the effect is not satisfactory.

Method used

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  • Microbial agent for preventing and controlling apple root rot and preparation method thereof
  • Microbial agent for preventing and controlling apple root rot and preparation method thereof
  • Microbial agent for preventing and controlling apple root rot and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Trichoderma harzianum (preservation number: CGMCC No.19037, the same below) and Trichoderma konshii (preservation number: CGMCC No.19038, the same below) were inoculated into the seed tank and fermented for 36 hours by conventional methods and then transplanted to the fermenter for 60 hours. The thalline fermentation liquid that obtains after hour, the thalline fermentation liquid containing mycelia dry weight is 2.8g / L, for subsequent use.

[0038] Get 50kg of wheat bran, 50kg of soybean meal powder, and 100kg of sawdust and mix them into the fermentation tank, add 10kg of urea+10kg of potassium dihydrogen phosphate to the 75kg of Trichoderma harzianum culture solution prepared above, stir evenly, and then mix them into the raw materials of the fermentation tank. Fermentation at 25-32° C. for 10 days to obtain a fermented product of Trichoderma harzianum;

[0039] Take 100kg of wheat bran, 50kg of soybean meal powder, and 100kg of sawdust and mix them into the fermentati...

Embodiment 2

[0047] Trichoderma harzianum and Trichoderma konshii were inoculated into seed tanks for 24 hours of fermentation according to conventional methods, and then transplanted to the fermentation tank for 48 hours to obtain the thalline fermentation liquid, the thalline fermentation liquid contains mycelium dry weight of 2.6g / L ,spare.

[0048] Get 100kg of wheat bran, 25kg of soybean meal powder, and 50kg of sawdust and mix them into the fermentation tank, add 5kg of urea+5kg of potassium dihydrogen phosphate to the 50kg of Trichoderma harzianum nutrient solution prepared above, stir evenly, and then mix them into the raw materials of the fermentation tank. Fermentation at 25-32° C. for 7 days to obtain a fermented product of Trichoderma harzianum;

[0049] Take 100kg of wheat bran, 25kg of soybean meal powder, and 50kg of sawdust and mix them into the fermentation tank, add 5kg of urea + 5kg of potassium dihydrogen phosphate into the 75kg of Trichoderma konii culture solution pre...

Embodiment 3

[0052] Trichoderma harzianum and Trichoderma konshii were inoculated into seed tanks and fermented for 36 hours according to conventional methods, and then transplanted to the fermenter for 72 hours to obtain the thalline fermentation liquid. The dry weight of mycelia contained in the thalline fermentation liquid was 2.9g / L ,spare.

[0053] Get 100kg of wheat bran, 50kg of soybean meal powder, and 100kg of sawdust and mix them into the fermentation tank, add 10kg of urea+5kg of potassium dihydrogen phosphate to the 60kg of Trichoderma harzianum culture solution prepared above, stir evenly, and then mix them into the raw materials of the fermentation tank. Fermenting at 25-32° C. for 8 days to obtain a fermented product of Trichoderma harzianum;

[0054] Take 100kg of wheat bran, 25kg of soybean meal powder, and 50kg of sawdust and mix them into the fermentation tank. Add 5kg of urea + 5kg of potassium dihydrogen phosphate to the 80kg of Trichoderma konshii culture solution pre...

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Abstract

The invention provides a microorganism for preventing and controlling apple root rot: Trichoderma harzianum HZD-5, Trichoderma koningii HZD-6, and also provides a microbial agent which is composed ofthe above two bacteria and used for preventing and controlling apple root rot. Research results show that the production method of the microbial agent is simple and the effect of preventing and controlling apple root rot is obvious; the microbial agent can effectively increase the disease resistance of apples; and there is no chemical residue due to addition of chemical drugs.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, in particular to a microbial bacterial agent for preventing and controlling apple root rot and a preparation method thereof. Background technique [0002] Apple root rot, also known as round spot root rot, is a kind of root rot disease with wide distribution and serious damage. Root rot is caused by the damage of Fusarium fungus habitual in the soil when the root system of apples is weak. According to the survey, the occurrence of apple tree root rot is closely related to the strength of the fruit tree root system. Drought, lack of fertilizer, soil salinization, soil erosion, soil compaction, poor ventilation, excessive fruiting, severe annual and severe pests and diseases are all important conditions for inducing the disease. Apple tree root rot is the main cause of apple production reduction. After the main root is infected, the early plants do not show symptoms. Later, with the inte...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14
Inventor 马建桥刘波孙波王芳宁芬
Owner SHANXI HAOZHIDA BIOLOGICAL SCI & TECH CO LTD
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