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Method for breeding locust

A technology for locust locust and pods, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low induction rate, difficult to meet the needs of locust reproduction and breeding, etc., and achieves high induction rate, high repeatability, and growth robust effect

Inactive Publication Date: 2012-12-26
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

The above-mentioned studies all use the immature zygotic embryos 1-4 after flowering as explants to induce culture, and the induction rate is low, the highest induction rate is only 12%, which is difficult to meet the needs of Robinia pseudoacacia propagation and breeding. Therefore, the establishment of Robinia pseudoacacia efficient Somatic embryogenesis system is imperative

Method used

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  • Method for breeding locust
  • Method for breeding locust
  • Method for breeding locust

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Embodiment 1

[0059] 1. Test materials

[0060] 1, the present invention takes the well-growing diploid Robinia pseudoacacia seedling population of Beijing Yanqing Mijiabao Nursery as a resource for drawing materials, and chooses 10 mother trees that grow well, have straight stems, and have a high seed setting rate (the interval between each strain is greater than 50 meters), between June and September 2010, collect the zygotic embryos of different developmental stages of Robinia pseudoacacia in the 4th to 12th week after flowering as explant test materials, collect once every 10 days, and collect 500 pods each time , that is, collect pods 25, 35, 45, 55, 65, and 75 days after flowering, collect 500 pods each time, store the pods collected on the same day in an ice box at low temperature and bring them back to the laboratory, and then put them in a refrigerator at 4°C Refrigerate for later use.

[0061] 2. Plant growth regulator

[0062] The plant growth regulating substance used in the p...

Embodiment 2

[0107] Except for the callus induction culture process, the 6-BA in the callus culture medium is 0 mg / L, and the 2,4-D is 5.0 mg / L; in the somatic embryo induction culture process, the somatic embryo induction medium The 6-BA used is 1.0 mg / L, the NAA is 1.0 mg / L, the rest is the same as that of Example 1, and the data analysis results are shown in Tables 2 and 3.

Embodiment 3

[0109] In addition to the callus induction culture process, the 6-BA in the callus culture medium is 0.3 mg / L, and the 2,4-D is 0.3 mg / L; in the somatic embryo induction culture process, the somatic embryo induction culture The used 6-BA of base is 0.1mg / L, and NAA is 0.1mg / L, and all the other are identical with embodiment 1, and data analysis result is as shown in table 2,4.

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Abstract

The invention discloses a method for breeding locusts, which uses zygotic embryo of different embryonic ages as explants, and uses MS+2, 4-D+BA+cane sugar+agar as an induction medium of an embryonic callus. The method comprises: using MS basal medium + MES + glutamine + casein hydrolysate + naphthylacetic acid + 6-Benzylaminopurine+cane sugar+agar as a somatic cell embryo medium, by inducting the callus, transferring the formed globular embryo onto MS+casein hydrolysate medium, carrying out maturation culture of somatic embryo, and bourgeoning the cultured somatic embryo on a basal medium to from a complete small plant. The method of the invention has the advantages of high callus inductivity, high somatic embryo inductivity and high germination rate, and can cultivate a large numbers of locust test-tube plantlets in a short period for large scale and factory production.

Description

technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for in vitro culture and plant regeneration of black locust, and belongs to the field of tissue culture of black locust. Background technique [0002] Black locust (Robinia pseudoacacia L.), also known as acacia, belongs to the genus Robinia L. of the subfamily Fabaceae (Leguminosae), a deciduous tree, native to the United States, and naturally distributed in the Appalachian Mountains in the eastern United States. .Mt.) and the Ozank Mountains (Ozank.Mt.), which were introduced into my country in the early 20th century and rapidly expanded and planted after the introduction, are the main timber forests and firewood forests in the middle and lower reaches of the Yellow River, the Huaihe River Basin, the Haihe River Basin, and the lower reaches of the Yangtze River. , water and soil conservation forests, seawall and river embankment protection forests, there are 4 billion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李云习洋王欢孙宇涵孙鹏袁存权李允菲戴丽胡瑞阳
Owner BEIJING FORESTRY UNIVERSITY
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