Tissue culture method of Gentiana dahurica Fisch for rapid in vitro reproduction

Abstract

The invention relates to a tissue culture method of Gentiana dahurica Fisch for rapid in vitro reproduction. The method comprises the following steps: (1) selection and sterilization process of explant, comprising taking the stem tip of seedling of biennial Gentiana dahurica Fisch in a greenhouse as the explant, dipping for sterilization and washing so as to obtain the sterilized explant stem tip; (2) inoculation of explant, comprising inoculating the explant stem tip after sterilization at a callus tissue induction culture medium so as to form callus tissue; (3) proliferation and differentiation culture of the callus tissue, comprising inoculating the callus tissue to a callus tissue proliferation culture medium to culture so as to form single buds of Gentiana dahurica Fisch; (4) induction of root growth, comprising separating the single buds of Gentiana dahurica Fisch and afterwards transplanting into the root growing culture medium to culture so as to form complete regenerated plantlets; (5) sterilization of seedling medium; (6) transplanting of plant, comprising transplanting the complete regenerated plantlets into the seedling substrate subjected to sterilization directly, and acclimating the seedling so as to grow normal Gentiana dahurica Fisch plants. According to the method, the problems of overlong seedling period and low reproduction coefficient of Gentiana dahurica Fisch are overcome, and the method is easy to operate and can be used for rapid factory seedling culture.

Description

technical field [0001] The invention relates to tissue culture and in vitro rapid propagation of plants, in particular to a method for in vitro rapid propagation of Gentiana miltiorrhiza tissue culture. Background technique [0002] Gentiana is a perennial herbaceous plant belonging to the genus Gentianaceae (Gentianaceae). It is a famous traditional Chinese and Tibetan medicinal material in my country. It is recorded in the 2005 edition of "Chinese Pharmacopoeia" and Tibetan Medicine Chronicles. It is distributed in alpine grasses at an altitude of 800-4500m. Meadows, thickets, forest margins, hillside grasslands, ravines and river beaches. In my country, Gentiana miltiorrhiza is mainly distributed in northern Sichuan, northwest, northwest, North China, northeast and other regions. It is a commonly used traditional Chinese and Tibetan medicinal material. The dried root is used as medicine and can be used to treat tonsillitis, urticaria and rheumatoid arthritis. . At present...

AI Technical Summary

Problems solved by technology

At present, the demand for Gentiana miltiorrhiza is increasing year by year. The medicinal materials of Gentiana miltiorrhiza have long relied on wild excavation to meet the needs of domestic and foreign markets. The germplasm resources have been severely damaged and the reserves have decreased sharply. The population of Gentiana miltiorrhiza has been destroyed, making its resources unable to meet the market. need