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Composite microbial fertilizer capable of preventing vegetable root-knot nematode disease and preparation method thereof

A technology of complex microbial bacteria and root-knot nematode disease, applied in the field of fertilizer manufacturing, can solve the problems of restricting the development and stable production of crops in protected areas, unreasonable nutrient distribution ratio, and inappropriate fertilization methods, etc., to prevent root-knot nematode disease, Labor saving, effect of improving soil permeability and oxygen supply

Inactive Publication Date: 2017-07-14
河南省农业科学院园艺研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, diseases caused by root-knot nematodes have seriously restricted the development and stable production of crops in protected areas in my country, affecting the sustained, rapid and healthy development of the national economy
[0003] Existing microbial organic fertilizers and microbial fertilizers, adding functional bacteria to prevent vegetable root-knot nematodes have general effects, and the amount of application is small, the method of fertilization is inappropriate, the ratio of nutrients in the matrix is ​​unreasonable, and the functional bacteria cannot be effectively supplied. Propagation and growth, the effect is not obvious, especially for soil-borne diseases, such as fusarium wilt, root-knot nematode, and almost no effect

Method used

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  • Composite microbial fertilizer capable of preventing vegetable root-knot nematode disease and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0030] The preparation of embodiment 1 functional bacterium raw material

[0031] (1) Cultivation of P. lilacensis strains

[0032] The Pl36-1-1 strain of P. lilacinus (preservation number is CCTCC NO: M2016683) was propagated with PDA medium (potato sucrose agar powder medium), cultured at 28°C for 7 days, and stored at 4°C.

[0033] Liquid fermentation, liquid medium PDB (potato sucrose medium), 28°C, 200rpm / min, cultivated for 9 days, and the spore content determined by the hemocytometer was 10 9 CFU / g. Store at 4°C.

[0034] (2) Proliferation of arbuscular mycorrhizal fungi (BGCAM0023)

[0035] First mix and sterilize the sand:soil at a ratio of 3:1, and then sterilize the arbuscular mycorrhizal fungi (National Resource Platform No. 1511C0001BGCAM0023, provided by the "Arbuscular Mycorrhizal Fungi Germplasm Resource Bank" of the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences) (BGC) ") and mixed sand at a ratio of 1:100 ...

Embodiment 2

[0038] Embodiment 2: a kind of compound microbial bacterial fertilizer that prevents root-knot nematode disease of vegetables, is made up of the raw material of following weight portion: 50 parts of chicken manure, 10 parts of straw powder, 4 parts of cornmeal, 2 parts of oil cake, 10 parts of functional bacteria, decomposed 1.5 parts of agent and 22.5 parts of water. The functional bacteria include P. lilacinus, arbuscular mycorrhizal fungi and Bacillus subtilis with a weight ratio of 4:1:5, and the live bacteria contents of the three raw materials are respectively 6×10 9 CFU / g, 5.1×10 9 CFU / gram of arbuscular mycorrhizal fungi, 9.3×10 9 CFU / gram.

[0039] The preparation method of the above-mentioned novel compound microbial bacterial fertilizer for preventing root-knot nematode disease of vegetables comprises the following steps: (1) fully mixing the chicken manure, straw powder, cornmeal, oil cake, decomposing agent and water in proportion, Compost at 55-60°C to kill p...

Embodiment 3

[0041]Embodiment 3: A kind of composite microbial fertilizer for preventing root-knot nematode disease of vegetables, is made up of the raw material of following parts by weight: 45 parts of chicken manure, 12 parts of straw powder, 5 parts of cornmeal, 1 part of oil cake, 12 parts of functional bacteria, decomposed 2 parts of agent and 23 parts of water. The functional bacteria include P. lilacinus, arbuscular mycorrhizal fungi and Bacillus subtilis with a weight ratio of 4:1:5, and the live bacteria contents of the three raw materials are respectively 6×10 9 CFU / g, 5.1×10 9 CFU / gram of arbuscular mycorrhizal fungi, 9.3×10 9 CFU / gram.

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Abstract

The invention relates to a composite microbial fertilizer capable of preventing the vegetable root-knot nematode disease and a preparation method thereof, and belongs to the field of fertilizer production. The composite fertilizer is produced by the following steps: adding carbon containing plant powder and cereal protein powder into a decomposed organic fertilizer to prepare an organic substance, adding functional bacteria into the organic substance, and fully and evenly mixing to obtain the novel multi-resistance composite fertilizer. The functional bacteria can breed massively around the roots of vegetables and crops to form circular protective covers, thus the diseases caused by root-knot nematode are prevented effectively, a good ecological environment is created for the growth of vegetables, at the same time, the soil environment is improved, the soil hardening is avoided, the ventilation and oxygen content of soil are improved, the product quality and output are increased, and the fertilizer has the functions of pesticide and fertilizer. The organic substance content of the composite microbial fertilizer is not less than 50%, and the beneficial bacterium content is 6*10<9> CFU / g. The composite microbial fertilizer is easy to use, the fertilizing effect is high, the fertilizer only needs one application, labor is saved, and the environment is protected.

Description

technical field [0001] The invention belongs to the technical field of fertilizer production, and in particular relates to a compound microbial fertilizer for preventing vegetable root-knot nematode disease and a preparation method thereof. Background technique [0002] Vegetable root-knot nematode is a kind of important plant pathogenic nematode that seriously damages economic crops and is widely distributed all over the world. It can infect not only economical food crops, vegetables and ornamental flowers and trees, but even weeds. Root-knot nematode disease can reduce the yield of commercial crops by 10-20%, and in severe cases, it can reach more than 75%, and even lead to crop failure. At present, the diseases caused by root-knot nematodes have seriously restricted the development and stable production of crops in protected areas in my country, and affected the sustained, rapid and healthy development of the national economy. [0003] Existing microbial organic fertili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05G3/02C05G3/00C12N1/14C12N1/20C12R1/645C12R1/125C05G3/60C05G3/80
CPCC05F3/00C05G3/00C05G3/60C05G3/80C12N1/14C12N1/20C05F5/002C05F11/00C05F11/08
Inventor 马凯杨凡史宣杰蔡毓新赵秀山赵肖斌唐艳领
Owner 河南省农业科学院园艺研究所
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