Composite microbial fertilizer capable of preventing vegetable root-knot nematode disease and preparation method thereof
A technology of complex microbial bacteria and root-knot nematode disease, applied in the field of fertilizer manufacturing, can solve the problems of restricting the development and stable production of crops in protected areas, unreasonable nutrient distribution ratio, and inappropriate fertilization methods, etc., to prevent root-knot nematode disease, Labor saving, effect of improving soil permeability and oxygen supply
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Embodiment 1
[0030] The preparation of embodiment 1 functional bacterium raw material
[0031] (1) Cultivation of P. lilacensis strains
[0032] The Pl36-1-1 strain of P. lilacinus (preservation number is CCTCC NO: M2016683) was propagated with PDA medium (potato sucrose agar powder medium), cultured at 28°C for 7 days, and stored at 4°C.
[0033] Liquid fermentation, liquid medium PDB (potato sucrose medium), 28°C, 200rpm / min, cultivated for 9 days, and the spore content determined by the hemocytometer was 10 9 CFU / g. Store at 4°C.
[0034] (2) Proliferation of arbuscular mycorrhizal fungi (BGCAM0023)
[0035] First mix and sterilize the sand:soil at a ratio of 3:1, and then sterilize the arbuscular mycorrhizal fungi (National Resource Platform No. 1511C0001BGCAM0023, provided by the "Arbuscular Mycorrhizal Fungi Germplasm Resource Bank" of the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences) (BGC) ") and mixed sand at a ratio of 1:100 ...
Embodiment 2
[0038] Embodiment 2: a kind of compound microbial bacterial fertilizer that prevents root-knot nematode disease of vegetables, is made up of the raw material of following weight portion: 50 parts of chicken manure, 10 parts of straw powder, 4 parts of cornmeal, 2 parts of oil cake, 10 parts of functional bacteria, decomposed 1.5 parts of agent and 22.5 parts of water. The functional bacteria include P. lilacinus, arbuscular mycorrhizal fungi and Bacillus subtilis with a weight ratio of 4:1:5, and the live bacteria contents of the three raw materials are respectively 6×10 9 CFU / g, 5.1×10 9 CFU / gram of arbuscular mycorrhizal fungi, 9.3×10 9 CFU / gram.
[0039] The preparation method of the above-mentioned novel compound microbial bacterial fertilizer for preventing root-knot nematode disease of vegetables comprises the following steps: (1) fully mixing the chicken manure, straw powder, cornmeal, oil cake, decomposing agent and water in proportion, Compost at 55-60°C to kill p...
Embodiment 3
[0041]Embodiment 3: A kind of composite microbial fertilizer for preventing root-knot nematode disease of vegetables, is made up of the raw material of following parts by weight: 45 parts of chicken manure, 12 parts of straw powder, 5 parts of cornmeal, 1 part of oil cake, 12 parts of functional bacteria, decomposed 2 parts of agent and 23 parts of water. The functional bacteria include P. lilacinus, arbuscular mycorrhizal fungi and Bacillus subtilis with a weight ratio of 4:1:5, and the live bacteria contents of the three raw materials are respectively 6×10 9 CFU / g, 5.1×10 9 CFU / gram of arbuscular mycorrhizal fungi, 9.3×10 9 CFU / gram.
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