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Anticancer active OPB (oligopeptidase B) oligopeptide and expression vector and application thereof

A technology of anti-cancer activity and expression vector, which is applied in the direction of medical preparations containing active ingredients, the use of vectors to introduce foreign genetic materials, peptide sources, etc., which can solve the problems of difficult synthesis of polypeptide molecules, difficult separation and purification, etc., and achieve low cost , easy operation, high output effect

Active Publication Date: 2016-05-25
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Problems in the prior art: (1) Bacterial system expression products may contain endotoxin, but this shortcoming can be solved by using a method for removing endotoxin
(2) The expression system based on DNA plasmid is difficult to synthesize too small polypeptide molecules, because it is difficult to separate and purify

Method used

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  • Anticancer active OPB (oligopeptidase B) oligopeptide and expression vector and application thereof
  • Anticancer active OPB (oligopeptidase B) oligopeptide and expression vector and application thereof
  • Anticancer active OPB (oligopeptidase B) oligopeptide and expression vector and application thereof

Examples

Experimental program
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specific Embodiment approach 1

[0033]Embodiment 1: An OPB oligopeptide with anticancer activity of this embodiment, the sequence of which is Gly-Asn-Lys-Lys-Trp-Glu-Gln-Lys-Gln-Val-Gln-Ile-Lys-Thr -Leu-Glu-Gly-Glu-Phe-Ser-Val-Thr-Met-Trp-Ser-Ser.

specific Embodiment approach 2

[0034] Specific embodiment 2: An OPB oligopeptide with anti-cancer activity of this embodiment, the oligopeptide or any part of the oligopeptide is used to prepare a drug for treating cancer.

specific Embodiment approach 3

[0035] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the oligopeptide or a part of the oligopeptide is used to prepare a medicine for treating cancer. Others are the same as in the second embodiment.

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Abstract

The invention relates to an anticancer active oligopeptide and an expression vector and application thereof, in particular to anticancer active OPB (oligopeptidase B) oligopeptide and an expression vector and application thereof. A sequence of the oligopeptide is Gly-Asn-Lys-Lys-Trp-Glu-Gln-Lys-Gln-Val-Gln-Ile-Lys-Thr-Leu-Glu-Gly-Glu-Phe-Ser-Val-Thr-Met-Trp-Ser-Ser. The oligopeptide is used to prepare anticancer drugs. The expression vector pMBP-2xOPB is constructed. A peptide fragment synthesized by using the OPB sequence can inhibit the growth and proliferation of tumor cells, and it is shown that the OPB-based peptide fragment and its derivatives are potential drugs for treating cancers.

Description

technical field [0001] The present invention relates to an oligopeptide with anticancer activity and its expression vector and application. Background technique [0002] YinYang1(YY1) is a multifunctional protein with the activity of promoting tumor cell growth. YY1 has an OPB (OncoproteinBinding) region (eg figure 1 shown), can bind to multiple proteins (oncoproteins) that promote cancer production and development, including AKT, Mdm2, E1A and Ezh2. [0003] The occurrence and development of cancer are accompanied by genetic and epigenetic changes. Epigenetics refers to those heritable traits that do not involve changes in DNA sequence during cell division. Epigenetics can coordinate the role of genetic information in the process of differentiation and development. Defects in these regulation lead to various diseases in humans, including cancer. YY1 is involved in many epigenetic regulatory mechanisms during tumorigenesis. To date, most studies have shown that the bio...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/70C12N15/66A61K38/17A61P35/00
CPCA61K38/00C07K14/47C12N15/66C12N15/70
Inventor 隋广超
Owner NORTHEAST FORESTRY UNIVERSITY