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A kind of preservation method of denitrifying bacteria

A technology of denitrifying bacteria and storage aids, applied in the direction of preservation of microorganisms, etc., can solve the problems of high storage cost and high energy consumption for long-term storage, and achieve the effects of high survival rate, economical and convenient storage, and reduced storage energy consumption

Active Publication Date: 2021-05-04
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method needs to add high-concentration nutrients and preservatives, and the preservation cost is relatively high.
In addition, the cryopreservation temperature is -20~-70°C. In order to improve the preservation effect, usually choose -50~-70°C, and the energy consumption for long-term storage is high.

Method used

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  • A kind of preservation method of denitrifying bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Prepare NO 2 - -N concentration is 200mg / L, glycerol is the carbon source, and the concentration is 2500mg / L in terms of COD. The denitrifying bacteria are cultivated in a 10L biological reaction tank with a temperature of 27°C and a pH of 7.8. The stirring speed is 20 rpm. When the bacteria entered the stable growth period, the culture was stopped, and the bacteria were collected after sedimentation and centrifugation.

[0029] Prepare the preserved nutrient solution, according to the mixture of denitrifying bacteria and preserved nutrient solution, the concentration of nutrients is: the concentration of glycerol is 1.0g / L in terms of COD, and the concentration of NO 2 - -N concentration is 0.2g / L, Fe 2+ The concentration is 0.02g / L, K + The concentration is 0.1g / L, Ca 2+ The concentration is 0.05g / L, Mg 2+ The concentration is 0.1g / L preparation.

[0030]Mix the bacterium with the prepared nutrient solution, add preservation aid A according to the concentratio...

Embodiment 2

[0032] The culture process and operating conditions are the same as in Example 1, except that the concentration of glycerol is 2.5 g / L in terms of COD. After 2 years, they were taken out to investigate the activity and survival rate of the bacteria. After 2 days of recovery, the activity of the bacteria can be completely restored, and the survival rate can reach 95%. When the amount of protective agent was increased, the preservation effect was basically the same.

Embodiment 3

[0034] The cultivation process and operating conditions are the same as those in Example 1, except that they are stored in a low-temperature refrigerator at -50°C. After 2 years, they were taken out to investigate the activity and survival rate of the bacteria. After 2 days of recovery, the activity of the bacteria can be completely restored, and the survival rate can reach 95%. The preservation effect is basically the same, but the energy consumption of -50 ℃ preservation is relatively high.

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Abstract

The invention relates to a method for preserving denitrifying bacteria, comprising (1) cultivating the denitrifying bacteria to a stable growth period, and collecting the bacteria; (2) preparing a nutrient solution for denitrifying bacteria preservation; (3) step (1) denitrifying bacteria The nitrifying bacteria are mixed with the preservation nutrient solution in step (2), and an appropriate amount of preservation aids are added, and the preservation aids include glycolipids, sugar alcohols and organic acid salts; (4) cryopreservation. Compared with the prior art, the method of the invention is suitable for the preservation of a large number of denitrifying bacteria, and has the advantages of high survival rate, quick recovery of activity, low storage energy consumption and the like.

Description

technical field [0001] The invention relates to a method for preserving bacteria, in particular to a simple and effective method for freezing and preserving denitrifying bacteria at low temperature. Background technique [0002] Ammonia nitrogen is a pollutant that the country realizes the total amount of water pollution control. Excessive ammonia nitrogen content in the water body will cause eutrophication of the water quality, resulting in the excessive reproduction of some algae in the water body, and the growth of other organisms will be affected, thus destroying the aquatic ecosystem and causing Water quality deteriorates and affects its function. Biological denitrification technology is currently a hot spot in the research and application of denitrification technology at home and abroad because of its environmental protection, high efficiency and no secondary pollution. Biological denitrification is to first oxidize ammonia nitrogen to nitrate nitrogen or nitrite nitr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 孙丹凤高会杰郭志华赵胜楠李宝忠陈明翔
Owner CHINA PETROLEUM & CHEM CORP
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