A kind of room temperature preservation method of denitrifying bacteria

A denitrifying bacteria, normal temperature technology, applied in the direction of preservation of microorganisms, can solve the problem of uneconomical and applicable

Active Publication Date: 2021-05-04
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cryopreservation temperature of this method is -20~-70°C, which is not economical and applicable to some bacteria that need short-term preservation.

Method used

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  • A kind of room temperature preservation method of denitrifying bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare NO 2 - -N concentration is 200mg / L, glycerol is the carbon source, and the concentration is 2500mg / L in terms of COD. The denitrifying bacteria are cultivated in a 10L biological reaction tank with a temperature of 27°C and a pH of 7.8. The stirring speed is 20 rpm. When the bacteria entered the stable growth period, the culture was stopped, and the bacteria were collected after sedimentation and centrifugation.

[0028] Prepare the preserved nutrient solution, according to the mixture of denitrifying bacteria and preserved nutrient solution, the concentration of nutrients is: the concentration of glycerol is 1.0g / L in terms of COD, and the concentration of NO 2 - -N concentration is 0.2g / L, Fe 2+ The concentration is 0.02g / L, K + The concentration is 0.1g / L, Ca 2+ The concentration is 0.05g / L, Mg 2+ The concentration is 0.1g / L preparation.

[0029] Mix the bacteria with the prepared nutrient solution, add preservation aid A according to the concentratio...

Embodiment 2

[0031] The culture process and operating conditions are the same as in Example 1, except that it is preserved at 0°C. After 12 months, it was taken out to investigate the activity and survival rate of the bacteria. After 10 hours of recovery, the activity of the bacteria can be fully recovered, and the survival rate can reach 95%.

Embodiment 3

[0033] The cultivation process and operating conditions are the same as those in Example 1, except that the preservation aid A is added according to the concentration of the preservative in the system at 1.0 mg / L. After 6 months, it was taken out to investigate the activity and survival rate of the bacteria. After 8 hours of recovery, the activity of the bacteria can be fully recovered, and the survival rate can reach 95%.

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Abstract

The invention relates to a normal temperature preservation method for denitrifying bacteria, comprising (1) cultivating the denitrifying bacteria to the logarithmic growth phase, and collecting the bacteria; (2) preparing a preservation nutrient solution, mainly including carbon source, nitrogen source and trace substances, etc.; (3) Step (1) denitrifying bacteria are mixed with step (2) preserved nutrient solution in a preservation container, and an appropriate amount of preservation aids are added, and the preservation aids include glycolipids, sugar alcohols and organic acid salts; (4 ) Seal the mixture in step (3) and incubate for 4-12 hours, and store it at room temperature. Compared with the prior art, the method of the invention is suitable for long-term storage of a large number of denitrifying bacteria at room temperature, and has the advantages of good storage effect at room temperature, high survival rate, quick recovery of activity and the like.

Description

technical field [0001] The invention relates to a method for preserving bacteria, in particular to a simple and effective method for preserving denitrifying bacteria at room temperature. Background technique [0002] Ammonia nitrogen is a pollutant that the country realizes the total amount of water pollution control. Excessive ammonia nitrogen content in the water body will cause eutrophication of the water quality, resulting in the excessive reproduction of some algae in the water body, and the growth of other organisms will be affected, thus destroying the aquatic ecosystem and causing Water quality deteriorates and affects its function. Biological denitrification technology is currently a hot spot in the research and application of denitrification technology at home and abroad because of its environmental protection, high efficiency and no secondary pollution. Biological denitrification is to first oxidize ammonia nitrogen to nitrate nitrogen or nitrite nitrogen through...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 孙丹凤高会杰郭志华赵胜楠李宝忠陈明翔
Owner CHINA PETROLEUM & CHEM CORP
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