Method for differentiating bone cells by oriented inducing chick embryo stem spermatogonium

A spermatogonial stem cell and osteoblast technology, which is applied in the field of directional induction of chicken embryo spermatogonial stem cells to differentiate into osteoblasts, and achieves the effect of strong operability, advanced and simple technology
CN1935986AInactive Publication Date: 2007-03-28YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2007-03-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses the method used to directionally induce chicken embryo stem spermatogonium to differentiate into bone cell. Its features are that inoculating the chicken stem spermatogonium to culture bottle without feeder layer; using conditioned medium to induce culturing which is that the DMEM high glucose contains 10% calf blood serum, 2% chicken blood serum, 2mmol / L L-glutamine, 1mmol / L sodium pyruvate, 5.5*10-15ng / ml beta-mercaptoethanol, 10 mul / ml non-essential amino acid, 10-7mol / L dexamethasone, 0.01mol / L beta-phospho glycerol, 0.05g / L vitamin C. The invention has the advantages of advanced technology, simple operation, strong repeatability, providing possible for realizing tissue organ rebirthing and replacing. Thereby hopefully to find out the approach for build cell platform in large scale production, further a new study prospect can be exploited in developmental biology, medicine, genetics fields etc.
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Description

technical field

[0001] The invention relates to a method for differentiating chicken embryo spermatogonial stem cells into osteoblasts, which belongs to cell engineering

[0002] technology field. Background technique

[0003] Chicken spermatogonial stem cells (SSCs) have the ability to differentiate into pluripotency, and adding appropriate differentiation-inducing agents during in vitro culture can induce the required special types of cells and tissues, which has attracted great attention. At present, people's research on the induction of stem cells into osteoblasts is mostly limited to mammals (rats, mice). Gopalakrish.V et al. used mouse bone marrow stromal stem cells (BMSCs) to induce osteoblasts. Study the internal mechanism of BMSCs induction of osteoblasts; Zhang Weicheng, Ding Lianghua, etc. respectively used rat and human bone marrow stromal cells to induce osteoblasts, and they all achieved success.

[0004] However, the research on poultry SSCs is still in its ...

Claims

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