Skin spermatogonium protection solution

Abstract

The invention discloses a skin spermatogonium protection solution. The specific technical scheme is that the skin spermatogonium protection solution is formed by mainly dissolving an aminoglycoside-based antibiotic by using a DMEM culture medium aqueous solution, wherein 1 mL of the DMEM culture medium aqueous solution can dissolve 50-200 units of the aminoglycoside-based antibiotic, the concentration of the DMEM culture medium aqueous solution is 10-25 g / L, preferably the aminoglycoside-based antibiotic is one or a plurality of materials selected from gentamicin, kanamycin, amikacin and tobramycin, and preferably the skin spermatogonium protection solution further comprises oxyhemoglobin, Tween 80, palmitic amide, potassium alginate, lauroyl sarcosine, citric acid, pyruvic acid, fumaric acid and acetyl-CoA. The skin spermatogonium protection solution of the present invention provides the suitable and stable preservation environment for skin spermatogonium, and can significantly improve the vitality of the preserved skin spermatogonium.

Description

technical field [0001] The invention belongs to the technical field of skin spermatogonia preservation, and in particular relates to a skin spermatogonia protective solution. Background technique [0002] At present, in my country's clinical and cell research experiments, the collection, long-term storage and use of cells are separated in time and space, and the collected cells have very strict requirements on the external environment after isolation, especially when cells are transported between cell collection and long-term storage. , because the cells have not been preserved for a long time, the external environment will reduce the viability of the cells, so adding the protective solution immediately after the cells are collected to maintain the viability of the cells has become a very important topic. Because the collected cells will eventually enter the human blood whether they are cultured, proliferated or preserved, the added protective solution must on the one hand ma...

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Problems solved by technology

[0003] The conventional cell protection solution is physiological saline, which is convenient and safe, but the disadvantage is that the protective effect is not good. According to the patent document CN101919380A, when using normal saline to protect mesenchymal stem cells, the viability of mesenchymal stem cells will decrease by about 10% after 2 hours, so physiological Saline is only suitable for immediate preservation of mesenchymal stem cells

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