Method for genetic targeting of stem spermatogonium of poultry

A spermatogonial stem cell and gene targeting technology, which can be applied in microorganism-based methods, genetic engineering, plant genetic improvement, etc., can solve the problem of low proliferation efficiency of spermatogonial stem cells, improve the homologous recombination efficiency of positive clones, and achieve high protein yield. , the effect of short homology arms
CN101575614AInactive Publication Date: 2009-11-11JINLING INST OF TECH +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JINLING INST OF TECH
Publication Date
2009-11-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for genetic targeting of stem spermatogonium of poultry. By using an adenovirus of poultry as a targeting vector, the method performs targeting operation on the stem spermatogonium of the poultry; the obtained genetically modified stem spermatogonium can be used for transplanting testis and obtaining a transgenetic gene; and by mating the normal poultry, the stem spermatogonium can be further used for breeding the transgenetic poultry. The method has the advantages that: the method can effectively realize the high-efficiency genetic targeting of the stem spermatogonium of the poultry; the used homologous arm is short, and the vector is easily established; and after the exogenous objective albumen is directly integrated in an albumin expression albumen high-efficiency promotor, the expressional specificity of the exogenous albumen of the transgenetic offspring is high, the yield of albumen is high. Moreover, the method also has the advantages of simple operation, safety, no pathogenicity, low immunogenicity and less cell toxins.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering and relates to a gene targeting method. Background technique

[0002] Compared with mammalian bioreactors, avian oviduct bioreactors have many advantages, such as simple and efficient, low cost, easy purification, safety and stability, and have great development potential and market application prospects. The concept of poultry as a bioreactor experiment has existed for thirty years, but in fact in 2004 (lentiviral vector method to produce transgenic poultry appeared, McGrew M J, Sherman A, Ellard F M, et al.EMBO, 2004, 5: 728 -733) before, people focused more on how to achieve effective gene transfer in poultry. After the emergence of the lentiviral vector method, it included the development of other technical conditions. Now, people really have the conditions to think more about exogenous The specificity and high efficiency of protein expression, from a practical point of view, the ...

Claims

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