117results about How to "Restore fertility" patented technology

Non-human animals, tissues, cells, and genetic material are provided that comprise a modification of an endogenous non-human heavy chain immunoglobulin sequence and that comprise an ADAM6 activity functional in a mouse, wherein the non-human animals express a human immunoglobulin heavy chain variable domain and a cognate human immunoglobulin λ light chain variable domain.

The invention discloses an organic fertilizer or a bio-mixed organic fertilizer special for rice and a preparing method thereof. The organic fertilizer or the bio-mixed organic fertilizer special for rice is prepared by composting and fermenting raw materials (dry basis) according to the following weight percentage: 10%-20% of straw stalks, 25%-30% of rice chaff (bran), 25%-35% of rapeseed cakes (dregs), 5%-20% of sugar refinery filtered mud, 5%-10% of bagasse ash and 0.1% of ferment bacterial fertilizer. The organic fertilizer or the bio-mixed organic fertilizer is good in fertilizer effect and environment-friendly, can lead nutritional components absorbed by rice, rape and sugarcanes from the soil to return to the land so as to restore soil fertility, leads circular economy to be developed, use quantity of fertilizers and pesticides are reduced, green and organic agricultural products are produced, production cost and environment cost are reduced, food safety is improved, environment is protected, and modern agriculture and green agriculture development are promoted.

The invention belongs to the field of soil conditioners and relates to an algae microbial soil conditioner specially used for saline-alkali soil. The algae microbial soil conditioner is prepared by blending the raw materials including an acidic raw material, a plant sourced organic substance, an animal sourced organic substance, a composite probiotic agent, an enzymolysed algae extract, and medium and trace elements. The soil conditioner is prepared by combining the probiotics and the algae active extract, so that on one hand, pH value of the saline-alkali soil can be adjusted, saline-alkali degree is reduced, root system growth environment of crops is improved and damage on a root system due to the salt and alkali is reduced; and on the other hand, available phosphorus, immobilized by soil, can be released, so that available microelements in soil are effectively supplied, thereby improving soil fertility. Meanwhile, alginic acid can improve the water retention and fertilizer saving capability of soil, thereby finally improving the soil completely, increasing crop quality and utilization rate of fertilizers, and achieving yield increasing.

The invention belongs to the field of phytoremediation of heavy metal polluted soil, and discloses a method for improving phytoremediation efficiency of copper-polluted soil on the basis of the existing phytoremediation method for the heavy metal polluted soil. The method comprises the following steps: applying a chemical enhancer containing powdered sulfur and ethylenediaminedisuccinic acid to the copper-polluted soil; spraying a nutrient solution containing exogenous plant hormone naming zeatin Z at a fixed period after planting elsholtzia splendens; picking the aboveground parts of elsholtzia splendens after the growing season is finished, so as to reach the purpose of remediating the heavy-metal copper polluted soil. With the adoption of the method, the growth of elsholtzia splendens is promoted, and the problems that high-copper soil represented by soil in a copper mine area inhibits the growth of elsholtzia splendens and causes short plant, small biomass and slow growth can be solved; the method has the advantages of being high in absorbing efficiency, high in degree of enrichment, and obvious in remediation effect.

This invention relates to a nuclear conditional male sterility system in Brassica napus. Embodiments of the invention provide for the (male sterile) prebasic female (MsMsrfrf), the (male fertile) maintainer line (msmsrfrf), the (male sterile) basic female line (Msmsrfrf), and hybrid lines. Further provided are methods for the production of those lines. Further embodiments of the invention relate to markers associated to the sterility, fertility and maintainer alleles and the use of those markers in providing a hybrid system.

The invention relates to the field of composite fertilizers, and in particular to a whole-element composite fertilizer and a preparation method thereof. The whole-element composite fertilizer consists of the following raw materials in parts by weight: urea, triple superphosphate, potassium sulfate, monoammonium phosphate, potassium chloride, ammonium molybdate, boric acid, polypeptide alkali chelate zinc, citrate chelate copper, long-lasting additives, illite powder, attapulgite powder and humic acid ammonium. The preparation method comprises the following steps: preparing pretreated raw materials, mixing and stirring, crushing cakes, pelletizing by using a pelletizer, drying granules, cooling the granules, screening, coating finished granules so as to obtain a finished product, and the like. The invention provides the whole-element composite fertilizer which is capable of effectively replenishing organic matters and various medium-trace elements which are urgently required by soil, improving soil structures, improving permeability, and promoting nutrient absorption of crops, and the invention further provides the reasonable preparation method for the whole-element composite fertilizer.

The invention provides a remediation method for organic contaminated soil. The remediation method comprises the following steps of: mixing the organic contaminated soil, persulfate and bio-carbon powder to obtain pre-remediated soil; and ageing the pre-remediated soil for 30-40 days to remediate the organic contaminated soil. The remediation method can effectively eliminate the organic pollutantsin soil, further can supplement organic carbon for the soil, reduces toxicity of the contaminated soil, remediates soil fertility, does not generate secondary pollution, realizes efficient, green, safe and complete remediation for the organic contaminated soil, and provides a novel way for efficient and safe remediation for the organic contaminated soil.

The invention relates to a wood charcoal kiln smoke gas treatment system, and belongs to the technical field of smoke gas treatment. The wood charcoal kiln smoke gas treatment system comprises graded dust removal equipment, a smoke gas exhaust chimney and a power distribution cabinet, wherein the graded dust removal equipment is connected with a wood charcoal kiln exhaust opening; the graded dust removal equipment structurally comprises a condensation processing device, an electrostatic adsorption device and a burner arranged at the outlet part of the chimney; a gas inlet and a gas outlet of the condensation processing device are respectively connected with the wood charcoal exhaust opening and the gas inlet of the electrostatic adsorption device; the gas outlet of the electrostatic adsorption device is connected with a gas inlet of the chimney through an induced draft fan and a pipeline. The electrostatic wood charcoal kiln smoke gas treatment equipment has the key characteristics that the matched use of three procedures and the sequential strict control are adopted; a condensation device is used as a first work procedure; the electrostatic adsorption device is used as a second work procedure; a smoke gas burning device is used as a third work procedure; wood charcoal smoke gas sequentially passes through the three work procedures; finally, the wood charcoal smoke gas is thoroughly purified.

The invention discloses an improved cabbage type oilseed rape radish cytoplasmic male sterility restoring line and an improvement method thereof. The improvement method comprises the following steps: by taking a cabbage type oilseed rape Ogura CMS sterile line as a female parent and taking a cabbage type oilseed rape Ogura CMS restoring line as a male parent, hybridizing to obtain generation F1 seeds; carrying out radiation treatment on the generation F1 seeds and sowing the seeds to obtain generation F1 plants after the radiation treatment; by taking the cabbage type oilseed rape Ogura CMS sterile line as a female parent and taking the generation F1 plants as a male parent, hybridizing to obtain seeds; sowing the seeds to obtain later-generation plants; and carrying out single-plant molecular marker screening on fertile plants in the later-generation plants to obtain an improved cabbage type oilseed rape Ogura CMS restoring line. According to the improved cabbage type oilseed rape radish cytoplasmic male sterility restoring line and the improvement method thereof, molecular markers, which are tightly interlocked with restoring genes, in exogenous radish chromosome segments are eliminated, and are screened to obtain the improved restoring line with relatively short exogenous radish chromosome segments; and the improved restoring line has a good agricultural shape and a relatively high grain yield.

The present invention provides for a device comprising a scaffold composition, a bioactive composition and a bio-in-hibiting composition, wherein said bioactive and bio-inhibiting compositions are incorporated into or coated onto said scaffold composition, wherein said scaffold composition temporally supports survival and growth of resident follicles, migration and multiplication of stroma cells and spreading and organization of endothelial cells and new vessels wherein said bioactive composition regulates development of a resident follicle, formation of new blood vessels and chemoattraction and proliferation of stroma cells and wherein the bio-inhibiting composition regulates inhibition of the development of a second resident follicle. The presence of the bio-inhibiting composition within the scaffold is involved in the quiescence of the follicles in the primordial stage, which is important to restore fertility.

The invention discloses a microorganism soil conditioner for saline-alkali soil. The microorganism soil conditioner is formed by the following raw materials according to the parts by weight: 20-30 parts of ectothiorhodospirasp fermented liquid, 5-10 parts of halobacterium jilantaiense fermented liquid, 10-20 parts of lactobacillus plantarum fermented liquid, 10-15 parts of trichoderma viride fermented liquid, 5-10 parts of pseudomonas fluorescens fermented liquid, 5-10 parts of sinorhizobium meliloti fermented liquid, 20-30 parts of an organic fertilizer, 0.5-1 part of humic acid, and 0.5-1 part of Grondverbeteraar. In addition, the invention further provides a preparation method of the microorganism soil conditioner for the saline-alkali soil. The soil conditioner is capable of improvingthe soil physical and chemical properties of the saline-alkali soil, and creating a soil environment suitable for the crop growth, and improving the soil fertility and the overground part biomass thereof. In addition, the soil conditioner uses the pure biological raw materials, is green and environmental, and has no hazard to the soil and the environment.

The present invention provides a method of growing spermatogonial stem cells of mammals and the like in vitro, which is characterized in that glial cell-derived neurotrophic factor (GDNF) or an equivalent thereto, and leukemia inhibitory factor (LIF) are contained in a medium (culture broth) for culturing spermatogonial stem cells. According to the method of the present invention, spermatogonial stem cells can be grown in vitro to the extent that enables use thereof for developmental engineering.

The invention relates to an organic fertilizer converted by waste silkworm faeces and a production method of the organic fertilizer. The production method comprises the steps of spreading the silkworm faeces to a spreading height of 10-15cm, mixing grenadine syrup with a weight percentage of 10%, lactic acid bacteria and yeast in the proportion of (250-350): (40-60):1, obtaining fermentation liquid after uniform stirring, uniformly spraying the fermentation liquid on the spread silkworm faeces till the silkworm faeces are wet through exactly, spreading a layer of silkworm faeces to a spreading height of 10-15cm again on the sprayed silkworm faeces, uniformly spraying the fermentation liquid till the silkworm faeces are wet through exactly, treading down, covering a film on the silkworm faeces, keeping for 15-30 days, turning, covering with a film for 10-15 days again, adding phosphorus and potassium to the silkworm faeces, with a weight ratio of silkworm faeces, phosphorus and potassium of 100:(3-6):(1-4), pelleting, drying, bagging, and then obtaining a finished product. The waste silkworm faeces are converted into the environment-friendly organic fertilizer, so that the problem that the silkworm faeces pollute the environment can be solved, endogenesis hormone secretion of plants can be stimulated, the tillering capacity of plant cells is high, the effective stems are more, the crop planting requires no nitrogenous fertilizer for prompting growth, and the quality is good.

The invention dis closes a nitrogen fixing straw decomposing inoculant capable of inhibiting a soil-borne disease. The nitrogen fixing straw decomposing inoculant capable of inhibiting the soil-borne disease mainly comprises the following materials: southern phanerochaete chrysosporium powder, streptomyces chibaensis powder, streptomyces melanosporofaciens powder, clostridium barati powder, beijerinckia fluminensis powder and asahii bacillus powder; the decomposing inoculant is prepared through complex fermentation of cellulose, lignin, the southern phanerochaete chrysosporium having a function of decomposing hemicellulose, the streptomyces chibaensis powder capable of inhibiting the soil-borne disease, the streptomyces melanosporofaciens powder, the clostridium barati having a strong nitrogen fixing capability, the beijerinckia fluminensis powder and the asahii bacillus powder. After the decomposing inoculant is applied in a field, the decomposing speed of a wheat straw is increased, the weight loss ratio of the wheat straw is remarkably increased, the tensile force of the wheat straw is remarkably reduced, the number of antagonistic bacillus and actinomycetes of soil pathogens is remarkably increased, the number of pathogenic microorganisms is remarkably reduced, and nitrogen-fixing bacteria are remarkably increased.

The invention relates to the technical field of mercury-contaminated soil remediation, in particular to a method for remedying mercury-contaminated soil of farmland through crops. The method comprises the steps that at the end of March or the beginning of April, a plurality pieces of farmland with the mercury-contaminated soil are selected, early-rice seedlings with developed root systems are selected to be transplanted, fertilizer and water management is conducted, microbial flora liquid is sprayed in the rice seedling stage, and harvesting is conducted in late July to early August; oilseed rape seeds are sown in the farmland in early September to early October in the current year, and oilseed rapes are uprooted after being ripen; ramie seedlings are transplanted to the farmland in a sunny day in late March to middle April in the next year, and first-stage ramie, second-stage ramie and third-stage ramie are harvested; and the steps are cyclically operated till the mercury content of the farmland soil reaches the national standard. According to the method, three crops such as the rice, the oilseed rape and the ramie which have the high enrichment effect on heavy metal mercury are sequentially planted on the farmland with the mercury-contaminated soil, remediation of the crops on the mercury-contaminated soil is promoted through a chemical regulation agent, and then the annual average purifying rate of mercury in the soil reaches up to 45%.

The invention discloses a method and a system for waste therophyte high-efficiency clean environment-protective cycled comprehensive utilization. In the method, waste therophyte is utilized to make machine-made charcoal, pyroligneous and woodtar in smoke gas generated in the charcoal making process are separated from the smoke gas by a separation device, the pyroligneous and the woodtar are taken as chemical materials, and the separated smoke gas is taken as fuel gas. The device comprises a charcoal making machine kiln set (1) and a vertical carbonization furnace set (2); the charcoal making machine kiln set (1) and the vertical carbonization furnace set (2) are connected with an inlet of a cooling and purifying system (4) through a pipeline (3); an outlet of the cooling and purifying system is connected with a gas storage holder (7) through a blower (5) and a water block (6) in sequence; and the gas storage holder is connected with a fuel gas generator (8) through the pipeline. The invention utilizes waste materials as raw materials for making charcoal, separates the chemical materials from waste smoke gas generated in the charcoal making process, uses the separated smoke gas as gas fuel, turns waste into treasure, effectively prevents pollution to the environment and has extremely good social and economic benefits.

The invention discloses an agricultural heavy metal polluted farmland soil remediation system, which is characterized in that the system comprises a soil quality detection system; the soil quality detection system comprises a fixed monitoring unit and a mobile detection unit; the soil quality detection system is connected with an information management system through wireless data, the information management system comprises an information processing center, and the information processing center comprises an information analysis center and a data storage library connected with the information analysis center and the soil quality detection system; the information management system is connected with a soil remediation system through wireless data, and the soil remediation system comprises vehicle-mounted soil remediation equipment; according to the invention, a real-time soil information map is made through the fixed and mobile detection monitoring terminal and the information processing center, an optimal soil remediation scheme is selected by means of accurate and timely data, and rapid, accurate and efficient remediation treatment on the soil polluted by heavy metals is realized by using a specially designed soil remediation device.

A soil improvement method comprises the steps of leaching or washing saline soil through water to remove redundant salt and alkali so as to obtain soil with the pH of 6-7; generating organic fertilizers through microbial population by means of organic matters; detecting contents of nitrogen, phosphorus, potassium and other microelements required by crop growth in the soil; uniformly infiltrating quantitative organic fertilizers and controlled-release chemical fertilizers into soil according to the microelement contents of the soil and demands of the planted crops. According to the soil improvement method, raw materials for producing the fertilizers can be collected easily, and manufacturing cost is low. Capacity of municipal refuse treatment and reutilization is improved greatly, pollution is reduced, and the environment is beatified. The produced fertilizers can improve a land structure, kill malignant bacteria in the soil, decompose high-concentration chemical components in the soil and avoid soil hardening. In addition, yield increase of the crops is promoted, and crop quality is improved.