34 results about "Elsholtzia splendens" patented technology

The invention relates to a technique of remediation of slightly and medio- metal-contaminated vegetable soil with production. The specific steps are as follows: firstly, Sedum alfredii and Elsholtzia splendens are intercropped in vegetable soil contaminated by heavy metal such as cadmium, copper and zinc in spring with a with inter plant distance of ten to fifteen centimeters, row spacing of thirty centimeters; secondly, the Sedum alfredii is harvested periodically, a growth period of Sedum alfredii is three to four months during a first harvest, from then on, the Sedum alfredii is harvested every three months or when growing to a height of thirty to forty centimeters, stems of a height of two to three centimeters are left above the ground in harvest; the Elsholtzia splendens is harvested in October; thirdly, a cadmium-enriched rape is planted after the harvest of the Elsholtzia splendens with the spacing in the row of fifteen to twenty centimeters and is harvested in April of the next year; fourthly, a cucumber is planted after the harvest of the rape with the spacing in the row of twenty to twenty-five centimeters, a cabbage is planted after the harvest of the cucumber in September, the Elsholtzia splendens is planted after the harvest of the cabbage, then a restoring period is completed. Adopting a super accumulator plant for absorbing a plurality of heavy metal from the soil, the invention achieves reducing the heavy metal content in the vegetables and guaranteeing the safety of the farm produce.

The invention provides a biological remediation method for soil polluted by cadmium, zinc, lead and copper ions. The biological remediation method is realized by the following technical scheme comprising the following steps: (1) selecting the soil to be remediated, polluted by heavy metals, manually removing weeds and shrubs, applying enough decomposed organic agricultural foreign fertilizers in the end of autumn and the beginning of winter, deeply ploughing to enable the soil to be weathered, freezing overwintering pests for death, ploughing a land thoroughly and crushing the soil by a rake; (2) after the soil is unfrozen in the spring, when the average temperature reaches more than 10 DEG C, transplanting woody and fast-growing trees, namely bamboo willows with the heavy-metal enriching capability; (3) after the transplanting is finished, when the temperature reaches more than 15 DEG C, implementing the earthworm remediating process, and then collecting earthworms; and (4) after the first-time collection of the earthworms in the earthworm remediating process, planting elsholtzia splendens and alfred stonecrop and then wholly removing the elsholtzia splendens plants and the alfred stonecrop plants with roots, and in the spring next year, reintroducing the earthworms, the elsholtzia splendens and the alfred stonecrop into the land for remediation again, wherein the whole remediation process lasts for 20 months totally.

The invention belongs to the field of phytoremediation of heavy metal polluted soil, and discloses a method for improving phytoremediation efficiency of copper-polluted soil on the basis of the existing phytoremediation method for the heavy metal polluted soil. The method comprises the following steps: applying a chemical enhancer containing powdered sulfur and ethylenediaminedisuccinic acid to the copper-polluted soil; spraying a nutrient solution containing exogenous plant hormone naming zeatin Z at a fixed period after planting elsholtzia splendens; picking the aboveground parts of elsholtzia splendens after the growing season is finished, so as to reach the purpose of remediating the heavy-metal copper polluted soil. With the adoption of the method, the growth of elsholtzia splendens is promoted, and the problems that high-copper soil represented by soil in a copper mine area inhibits the growth of elsholtzia splendens and causes short plant, small biomass and slow growth can be solved; the method has the advantages of being high in absorbing efficiency, high in degree of enrichment, and obvious in remediation effect.

The invention discloses a comprehensive heavy metal contaminated soil remediation method. A comprehensive phytoremediation technology combining woody fast-growing tree paulownia elongate with an obvious effect of fixing multiple heavy metals such as Cu, Pb, Cd and Zn and herbaceous plant Elsholtzia splendens with hyperaccumulating effect and high growth speed on Cu2+ is adopted, and an auxiliary animal (earthworm) restoration technology is used, so that the highest effect of eliminating heavy metal pollution of soil is achieved, in situ bioremediation of the soil is successfully realized, pollutants are effectively prevented from directly entering a food chain, and secondary pollution of the soil is avoided. The comprehensive heavy metal contaminated soil remediation method disclosed by the invention is environment-friendly, low in cost and easy to popularize in large area, has an obvious effect of remediating the heavy metal contaminated soil and can improve the fertility of the heavy metal contaminated soil.

The invention provides a biological remediation method for soil polluted by heavy metals. The biological remediation method comprises the following steps: selecting the soil, to be remediated, polluted by the heavy metals, manually removing weeds and shrubs, applying enough decomposed organic agricultural foreign fertilizers in the end of autumn and the beginning of winter, deeply ploughing to enable the soil to be weathered, freezing overwintering pests for death, ploughing a land thoroughly and crushing the soil by a rake; after the soil is unfrozen in the spring, when the average temperature reaches more than 10 DEG C, transplanting woody and fast-growing trees, namely bamboo willows with heavy-metal enriching capability; after the transplanting is finished, when the temperature reaches more than 15 DEG C, implementing the earthworm remediating process, and then collecting earthworms; and after the first-time collection of the earthworms in the earthworm remediating process, planting a herbaceous plant, namely elsholtzia splendens with hyperaccumulating action to Cu<2+> and high growth speed so as to enhance the enriching action to copper ions of the shallow soil, and then wholly removing the elsholtzia splendens plants with roots. The biological remediation method provided by the invention has the advantages that not only can the effect of removing the heavy-metal pollution of the soil maximally be achieved, but also the secondary pollution to the soil is avoided.

The invention discloses a biological heavy metal contaminated soil remediation method which comprises the following steps: (1) manually removing weeds and shrubs on to-be-remediated heavy metal contaminated soil, applying sufficient organic farm manures which are thoroughly decomposed at the season when autumn changes into winter, performing deep ploughing, so that the soil is weathered, overwintering injurious insects are frozen to death, and the land is thoroughly ploughed and harrowed; (2) transplanting woody fast-growing trees bamboo willow when the average air temperature reaches over 10 DEG C after the soil in spring is unfrozen; (3) introducing earthworms for soil remediation when the air temperature reaches over 15 DEG C after transplanting, and collecting the earthworms; (4) introducing Elsholtzia splendens and dayflower after the earthworms are collected for the first time in the earthworm remediation process, and wholly removing the Elsholtzia splendens plants and dayflower with roots; and (5) introducing the earthworms, Elsholtzia splendens and dayflower again in spring in the next year, applying sufficient organic farm manures before introduction, thoroughly ploughing and harrowing the land, and repeating the previous processes. According to the method disclosed by the invention, the animals and plants are combined to act, soil remediation is realized, and the method is economic, environment-friendly, high in remediation efficiency and easy to popularize.

The invention relates to a method for using the refinishing plant elsholtzia to prepare the organic fertilizer with copper. It harvests, dries, shatters and piles the elsholtzia when it grows on 165-195 days; it amplifying cultivates the microorganism 1 and adds it into the pile and ascertains the viable bacteria number; it mixing cultivates the microorganism 2 and the molasses and adds it into the pile. The bacteria adding is 10% of the pile quality and the bacteria number is between 2.0x106CFU / g to 2.8x106CFU / g. It shifts the pile to guarantee the gas blow; it forms the organic fertilizer; the microorganism 1 is Cellulobacillus; the microorganism 2 is Effective Microorganisms. The OD value is between 0.5-0.6; the cellulose decomposing bacteria: the EM bacterium is 1:1.

The invention discloses a method for remediating heavy metal polluted soil comprehensively. A comprehensive plant remediation technology for combining woody fast-growing tree paulownia elongate with an obvious fixing effect to multiple kinds of heavy metals including Cu, Pb, Cd, Zn and herbaceous plant elsholtzia splendens with hyperaccumulation effect and rapid growth speed to Cu<2+> is adopted, and meanwhile an animal (earthworm) remediation technology is assisted so as to reach an effect of removing soil heavy metal pollution maximally, so that the in-situ bioremediation to soil is realized; moreover, pollutants are effectively prevented from entering a food chain directly so as not to generate secondary pollution to soil. The method is a comprehensive remediation method of heavy metal contaminated soil, which has good environment friendliness, is low in cost, has an obvious effect to the remediation of heavy metal contaminated soil, can enhance the fertility of the heavy metal contaminated soil, and is easy to popularize in a large area.

The invention relates to a plant source pesticide used for planting fruits and vegetables. The plant source pesticide is composed of the following raw materials: ethanol or chloroform extracts of air potatoes, gamboge, sophora flavescens, persimmon leaves, birch barks, gingko leaves and lavender, capsaicine, cinnamomum appelianum schewe oil, elsholtzia splendens nakai oil and vitex canescens oil. The extracts of the air potatoes, the gamboge, sophora flavescens, the persimmon leaves, the birch barks, the gingko leaves and the lavender, the capsaicine, the cinnamomum appelianum schewe oil, the elsholtzia splendens nakai oil and the vitex canescens oil are matched so that the plant source pesticide has the insect killing function of a bactericide, has no pollution to soil and environment for planting strawberries and has no residues on melons and fruits, and vegetables, and is safe to use.

The invention discloses a method for extracting an antitumor component caffeic acid from Elsholtzia splendens, which sequentially comprises the following steps of: 1) performing crude extraction, namely soaking flowering branches and leaves of the Elsholtzia splendens in ethanol and performing other treatment to obtain a thin extract; 2) performing extraction, namely dissolving the thin extract in water, extracting by using petroleum ether, and extracting the obtained water layer by using ethyl acetate to obtain an ethyl acetate extract; 3) separating the ethyl acetate extract by using macroporous resin to obtain a yellowish-brown extract; and 4) purifying and refining the yellowish-brown extract by using thin-layer silica gel, and using an elution system consisting of CH2Cl2, CH3OH and HCOOH in a volume ratio of 30:1:1 to obtain the caffeic acid. By the method, the high-purity caffeic acid can be obtained at high yield.

The invention discloses a method for extraction and separation of rosmarinic acid, apigenin and luteolin in elsholtzia splendens. The method comprises the steps of: (1) ethanol immersion extraction: performing air drying, smash, immersion extraction and extraction on branches and leaves of elsholtzia splendens harvested in the full-bloom stage to obtain a brownish black extractum; (2) performing macroporous resin coarse separation and concentration on the brownish black extractum to obtain a yellowish-brown extractum; (3) performing polyamide column separation on the obtained yellowish-brown extractum to obtain an extractum A, an extractum B and an extractum C; and (4) performing silica-gel column chromatography purification and refinement on the extractum A, the extractum B and the extractum C to obtain the apigenin, the apigenin and the rosmarinic acid correspondingly. Highly purified rosmarinic acid, apigenin and luteolin can be obtained by adopting the method provided in the invention.

The invention discloses a method for planting rhizoma polygonati. The method comprises following steps: land selection, land preparation, rhizome selection and treatment, rhizome planting, field management and harvesting. In the treatment of rhizomes, potassium hydroxide solution is first used to sterilize the rhizomes, and then self-made treatment liquid is used to soak the rhizomes and further sterilize the rhizomes, so as to improve the antibacterial ability of rhizomes. The cortex moutan in the self-made treatment liquid contains gallic acid, which has a strong inhibitory effect on variousfungi such as Bacillus subtilis and Proteus. The composition contains grapefruit peel, elsholtzia splendens leaf and pearl plum, and the volatile oil of the three also has a strong smoke-killing effect on pests and colonies. The rhizome of Polygonatum sibiricum is sterilized with potassium hydroxide solution and then soaked in self-made treatment liquid to kill harmful bacteria and microorganismscarried by rhizome of Polygonatum sibiricum, which greatly enhanced its antibacterial ability, avoided the rot and mildew of rhizome, and increased the rate of seedling emergence.

The invention discloses application of total Elsholtzia splendens Nakai flavones extract of copper-polluted soil in the preparation of a vascular dilatation medicine. The vascular dilatation effect of the total Elsholtzia splendens Nakai flavones is obviously superior to that of total Hangzhou white chrysanthemum flavones extract, total ginkgo flavones and total soybean flavones.

The invention belongs to the technical field of garden biology, and particularly relates to a seedling raising method of elsholtzia splendens nakai. According to the method provided by the invention,seeds are harvested by adopting close-to-mature elsholtzia splendens nakai seed spikes; seed loss caused by ejection when the seed spikes mature can be avoided; the problem of difficult collection ofthe elsholtzia splendens nakai seeds is solved; the post-maturation of the elsholtzia splendens nakai seed spikes is promoted by adopting indoor artificial environment treatment, thereby guaranteeingthe maturity of the seeds; at the same time, the germination of the elsholtzia splendens nakai seeds is induced by adopting a self-made pregermination agent, so that the problem of low germination rate of the seeds under natural conditions is solved; by adopting the method provided by the invention, the average seedling rate of the elsholtzia splendens nakai is 99.87%, and the effect is significant; post-maturation treatment of the elsholtzia splendens nakai seed spikes, induction of seed germination, and seedling cultivation do not require special facilities, drugs used are conventional reagents and are cheap and easy to obtain, and the cost is low.

A phytoremediation method for zinc-cadmium composite polluted soil is characterized in that the sedum with ore is cultivated in the zinc-cadmium composite contaminated soil area to be treated, and the harvested sedum with ore is harvested and transferred every year. In order to achieve the purpose of efficient management, in terms of cultivation and management, it can be harvested twice a year in July and November; it can also be used as an intercropping of Sedum sedum and Haizhou Sedum.

The invention discloses a botanical pesticide capable of preventing myzus persicae and an application of the botanical pesticide. The botanical pesticide is a composition which is prepared from appelianum oil, elsholtzia splendens oil and Vitex canescens oil, is added with an accessory ingredient to prepare a missible oil agent, a microemulsion or an emulsion in water. The botanical pesticide has a remarkable prevention effect on the myzus persicae, has no residue on tobacco leaves, improves the quality of the tobacco leaves, is harmless to the environment, natural enemies of the myzus persicae, people and livestock and has the strong practicability.

The invention provides a plant mixed cultivation remediation method for phthalate-polycyclic aromatic hydrocarbon-heavy metal polluted soil, and belongs to the technical field of soil remediation. Theplant mixed cultivation remediation method for the phthalate-polycyclic aromatic hydrocarbon-heavy metal polluted soil comprises the step of planting alfalfa, elsholtzia splendens and sedum plumbizincicola on contaminated soil through mixed cultivation. The plant mixed cultivation remediation method for the phthalate-polycyclic aromatic hydrocarbon-heavy metal polluted soil, provided by the invention, is simple and convenient to operate, can treat various pollutants in the soil by using the plants planted through mixed cultivation, and has the advantages of ecological environmental protectionand rapid soil remediation.

The invention discloses a botanical pesticide capable of preventing myzus persicae and an application of the botanical pesticide. The botanical pesticide is a composition which is prepared from appelianum oil, elsholtzia splendens oil and Vitex canescens oil, is added with an accessory ingredient to prepare a missible oil agent, a microemulsion or an emulsion in water. The botanical pesticide has a remarkable prevention effect on the myzus persicae, has no residue on tobacco leaves, improves the quality of the tobacco leaves, is harmless to the environment, natural enemies of the myzus persicae, people and livestock and has the strong practicability.

The invention relates to a technology for repairing vegetable field soil slightly polluted by heavy metals while producing. The steps are as follows: 1) Intercropping Sedum sedum and Sedum haizhou in the vegetable soil polluted by heavy metals of cadmium, copper and zinc in spring, the planting density is 10-15cm, and the row spacing is 30cm; 2) Harvest Sedum sedum regularly, the first time Harvest the southeast sedum for 3-4 months of growth, and then harvest once every 3 months or when it grows to a height of 30-40cm. When harvesting, the stem above the ground is 2-3cm high; Harvest Haizhou Xiangru in October; 3) Haizhou Plant cadmium-enriched rapeseed after Harvestifolia harvested, with a plant spacing of 15-20cm, and harvest in April of the following year; 4) After rapeseed, plant cucumbers with a plant spacing of 20-25cm, plant cabbage after cucumber harvest in September, and plant Haizhou after cabbage harvest Rosacea, complete a repair cycle. The invention utilizes super-accumulating plants to take away a large amount of heavy metals from soil, thereby reducing the content of heavy metals in vegetables and ensuring the safety of agricultural products.

The present invention discloses a preparation technology of crystal sugar garlics, and belongs to the field of the garlic processing. The preparation technology comprises the steps that the garlics of which outer skins and root hairs are removed are washed to be clean, the washed garlics are drained, then the drained garlics are mixed evenly with edible salt, the salt mixed garlics are loaded into a jar to be pickled, wherein the weight ratio of the garlics to the edible salt is (20:1) to (25:1), and the pickling time is 50-70 hours; during the pickling of the garlics, elsholtzia splendens is put into boiled water to be decocted for 5-10 hours at the same time, and one or two of white granulated sugar and crystal sugar is added into the decocted elsholtzia splendens water solution; the pickled garlics are put into the cooled elsholtzia splendens water solution to be soaked for 10-15 days in a seal way; the soaked garlics are drained, then the drained garlics are mixed evenly with white granulated sugar, the treated garlics are loaded into the jar, the jar is sealed and allowed to stand, jar reversing is conducted once after 4-7 days of the standing, the total standing time is 45-60 days, and the finished products are obtained. The elsholtzia splendens is introduced, so that the spicy taste and smell of the garlics are reduced. The traditional processing steps are optimized, so that the salt content of the crystal sugar garlics is greatly reduced and the mouthfeel of the crystal sugar garlics is improved.

The invention belongs to the field of garden biotechnology, and particularly relates to an artificial germination accelerating method for elsholtzia splendens seeds. The artificial germination accelerating method has the advantages that nearly-matured elsholtzia splendens ears are selected to harvest the seeds, seed loss caused by ejection when the ears are matured can be avoided, and the problemof difficulty in collection of the elsholtzia splendens seeds can be solved; indoor artificial environment treatment is adopted to promote after-ripening of the elsholtzia splendens ears, and the maturity of the seeds is ensured; a self-made germination accelerating agent is used to induce germination of the elsholtzia splendens seeds, so that the problem of low seed germination rate under naturalconditions is solved, and the germination rate of the elsholtzia splendens seeds is near 98%; according to the method, no special facilities are needed for after-ripening treatment of the elsholtziasplendens ears or inducing of seed germination, and drugs used are conventional reagents which are cheap, easy to obtain and low in cost.

The invention relates to mongollian thyme herb essential oil mosquito repellent floral water and a preparation method. The floral water is prepared from, by weight, 0.5-5 parts of mongollian thyme herb essential oil, 0.1-0.8 part of elsholtzia splendens essential oil, 3-8 parts of honeysuckle extracting solution, 3-8 parts of dandelion-viola mandshurica extracting solution, 0.1-0.3 part of glycerin and 0.1-0.3 part of glyceryl monostearate. According to the mongollian thyme herb essential oil mosquito repellent floral water and the preparation method, green, natural and pollution-free mongollian thyme herb is used as a raw material, the essential oil is extracted, and the mongollian thyme herb essential oil mosquito repellent floral water is prepared. The mongollian thyme herb essential oil and the elsholtzia splendens essential oil are used as mosquito repellent active components, honeysuckle flowers are used as a detumescence antipruritic active component, under the compound effect of the elsholtzia splendens essential oil, dandelions and viola mandshurica, the mosquito repellent effect of the mongollian thyme herb essential oil is greatly improved, the irritation to the skin isavoided at the same time, skin drying is avoided, and meanwhile the heat clearing and toxicity removing effects of removing swelling and tingling due to mosquito bites are further achieved.

The invention discloses a biological heavy metal contaminated soil remediation method which comprises the following steps: (1) manually removing weeds and shrubs on to-be-remediated heavy metal contaminated soil, applying sufficient organic farm manures which are thoroughly decomposed at the season when autumn changes into winter, performing deep ploughing, so that the soil is weathered, overwintering injurious insects are frozen to death, and the land is thoroughly ploughed and harrowed; (2) transplanting woody fast-growing trees bamboo willow when the average air temperature reaches over 10 DEG C after the soil in spring is unfrozen; (3) introducing earthworms for soil remediation when the air temperature reaches over 15 DEG C after transplanting, and collecting the earthworms; (4) introducing Elsholtzia splendens and dayflower after the earthworms are collected for the first time in the earthworm remediation process, and wholly removing the Elsholtzia splendens plants and dayflower with roots; and (5) introducing the earthworms, Elsholtzia splendens and dayflower again in spring in the next year, applying sufficient organic farm manures before introduction, thoroughly ploughing and harrowing the land, and repeating the previous processes. According to the method disclosed by the invention, the animals and plants are combined to act, soil remediation is realized, and the method is economic, environment-friendly, high in remediation efficiency and easy to popularize.

The invention relates to a method for using the refinishing plant elsholtzia to prepare the organic fertilizer with copper. It harvests, dries, shatters and piles the elsholtzia when it grows on 165-195 days; it amplifying cultivates the microorganism 1 and adds it into the pile and ascertains the viable bacteria number; it mixing cultivates the microorganism 2 and the molasses and adds it into the pile. The bacteria adding is 10% of the pile quality and the bacteria number is between 2.0x106CFU / g to 2.8x106CFU / g. It shifts the pile to guarantee the gas blow; it forms the organic fertilizer; the microorganism 1 is Cellulobacillus; the microorganism 2 is Effective Microorganisms. The OD value is between 0.5-0.6; the cellulose decomposing bacteria: the EM bacterium is 1:1.

The invention discloses a method for extracting and separating nepeta glucoside, homoplantaginin and apigenin glucoside from elsholtzia splendens. According to the invention, ethanol leaching / solution extraction, macroporous resin crude separation, gel column screening, and polyamide column separation purification are adopted; and semi-preparative liquid chromatography is also adopted for refining. With the method, nepeta glucoside, homoplantaginin and apigenin-7-o-beta-D-glucoside products are separated from the floral leaves of the environment-specific plant elsholtzia splendens. Nepeta glucoside and homoplantaginin are both extracted from elsholtzia splendens plant for a first time.

The invention provides a biological remediation method for soil polluted by cadmium, zinc, lead and copper ions. The biological remediation method is realized by the following technical scheme comprising the following steps: (1) selecting the soil to be remediated, polluted by heavy metals, manually removing weeds and shrubs, applying enough decomposed organic agricultural foreign fertilizers in the end of autumn and the beginning of winter, deeply ploughing to enable the soil to be weathered, freezing overwintering pests for death, ploughing a land thoroughly and crushing the soil by a rake; (2) after the soil is unfrozen in the spring, when the average temperature reaches more than 10 DEG C, transplanting woody and fast-growing trees, namely bamboo willows with the heavy-metal enriching capability; (3) after the transplanting is finished, when the temperature reaches more than 15 DEG C, implementing the earthworm remediating process, and then collecting earthworms; and (4) after the first-time collection of the earthworms in the earthworm remediating process, planting elsholtzia splendens and alfred stonecrop and then wholly removing the elsholtzia splendens plants and the alfred stonecrop plants with roots, and in the spring next year, reintroducing the earthworms, the elsholtzia splendens and the alfred stonecrop into the land for remediation again, wherein the whole remediation process lasts for 20 months totally.

The invention provides a biological remediation method for soil polluted by heavy metals. The biological remediation method comprises the following steps: selecting the soil, to be remediated, polluted by the heavy metals, manually removing weeds and shrubs, applying enough decomposed organic agricultural foreign fertilizers in the end of autumn and the beginning of winter, deeply ploughing to enable the soil to be weathered, freezing overwintering pests for death, ploughing a land thoroughly and crushing the soil by a rake; after the soil is unfrozen in the spring, when the average temperature reaches more than 10 DEG C, transplanting woody and fast-growing trees, namely bamboo willows with heavy-metal enriching capability; after the transplanting is finished, when the temperature reaches more than 15 DEG C, implementing the earthworm remediating process, and then collecting earthworms; and after the first-time collection of the earthworms in the earthworm remediating process, planting a herbaceous plant, namely elsholtzia splendens with hyperaccumulating action to Cu<2+> and high growth speed so as to enhance the enriching action to copper ions of the shallow soil, and then wholly removing the elsholtzia splendens plants with roots. The biological remediation method provided by the invention has the advantages that not only can the effect of removing the heavy-metal pollution of the soil maximally be achieved, but also the secondary pollution to the soil is avoided.

The invention discloses application of total Elsholtzia splendens Nakai flavones extract of copper-polluted soil in the preparation of a vascular dilatation medicine. The vascular dilatation effect of the total Elsholtzia splendens Nakai flavones is obviously superior to that of total Hangzhou white chrysanthemum flavones extract, total ginkgo flavones and total soybean flavones.