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Method for extracting antitumor component caffeic acid from Elsholtzia splendens

A caffeic acid and anti-tumor technology, which is applied in the field of biological resource utilization of natural products, can solve the problems such as the separation and purification of active ingredient caffeic acid that has not been reported, and achieve the effects of simple and safe extraction process, high yield and simple extraction process.

Inactive Publication Date: 2011-11-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the technology patent of extracting caffeic acid with high yield (>0.09%) and high purity (>98%) from plants in China, and there is no report on the method of isolating and purifying the active ingredient caffeic acid from the repairing plant Herba chinensis.

Method used

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  • Method for extracting antitumor component caffeic acid from Elsholtzia splendens
  • Method for extracting antitumor component caffeic acid from Elsholtzia splendens
  • Method for extracting antitumor component caffeic acid from Elsholtzia splendens

Examples

Experimental program
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Embodiment 1

[0037] Embodiment 1: A method for extracting caffeic acid, an anti-tumor component in Herba Cinnamomi, followed by the following steps:

[0038] 1), rough extraction:

[0039] Grind into dry powder (moisture content is lower than 0.5%, weight ratio) after air-drying the flower branches and leaves of Herba Cinnamomi harvested in the full bloom stage, pass through a 100 mesh sieve, take the above 500g dry powder, place it in a 10L plastic bucket, add 95 % (V / V) ethanol 5L, stirred evenly and soaked for 3 days until the material was completely imbibed, treated with 40KHz ultrasonic frequency for 1 hour, and suction filtered to obtain the filtrate.

[0040] Repeat the above-mentioned process for a total of 3 times with the residue obtained by suction filtration (that is, replace the dry powder with the residue, and repeat the above-mentioned leaching).

[0041] Combine the filtrate obtained from 4 extractions; then concentrate in vacuo at 45°C to obtain 126.1 g of dilute extract ...

Embodiment 2

[0065] Embodiment 2: A method for extracting caffeic acid, an anti-tumor component in Rhizoma pilosula, followed by the following steps:

[0066] 1), rough extraction:

[0067] Air-dry the flowering branches and leaves of Rhizoma haizhou harvested at the full bloom stage and grind them into dry powder, pass through a 100-mesh sieve, take the above 5000g dry powder, put it in a 100L plastic bucket, add 75L of 95% (V / V) ethanol, and stir well Soak for 4 days until the material is completely imbibed, treat with an ultrasonic frequency of 40KHz for 1 hour, and filter with suction to obtain a filtrate.

[0068] Repeat the above-mentioned process for a total of 3 times with the residue obtained by suction filtration (that is, replace the dry powder with the residue, and repeat the above-mentioned leaching).

[0069] Combine the filtrate obtained from 4 extractions; then concentrate in vacuo at 45°C to obtain 1275.1g of dilute extract ①. Yield 25.50%.

[0070] 2), extraction:

[...

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Abstract

The invention discloses a method for extracting an antitumor component caffeic acid from Elsholtzia splendens, which sequentially comprises the following steps of: 1) performing crude extraction, namely soaking flowering branches and leaves of the Elsholtzia splendens in ethanol and performing other treatment to obtain a thin extract; 2) performing extraction, namely dissolving the thin extract in water, extracting by using petroleum ether, and extracting the obtained water layer by using ethyl acetate to obtain an ethyl acetate extract; 3) separating the ethyl acetate extract by using macroporous resin to obtain a yellowish-brown extract; and 4) purifying and refining the yellowish-brown extract by using thin-layer silica gel, and using an elution system consisting of CH2Cl2, CH3OH and HCOOH in a volume ratio of 30:1:1 to obtain the caffeic acid. By the method, the high-purity caffeic acid can be obtained at high yield.

Description

technical field [0001] The invention belongs to the field of biological resource utilization of natural products, and relates to a method for separating and purifying the antibacterial and antitumor active ingredient caffeic acid in the repairing plant—Cheria haizhouensis. Background technique [0002] Elsholtzia splendens (Elsholtzia splendens), also known as copper grass, is a plant of the genus Elsholtzia in the family Lamiaceae. It has long been designated as the basic plant of Elsholtzia splendens in my country, but it was revised in the 1995 edition of the Pharmacopoeia. Folks use Haizhou incense more widely, and its effect is similar to that of fenugreek. As early as 1953, academician Xie Xuejin and Mr. Xu Bangliang, well-known exploration geochemists, discovered and reported that Rhizoma haizhou is an indicator plant of copper ore, and it is an effective indicator plant for copper ore mining in my country. In recent years, Chinese scholars have conducted a large num...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C59/52C07C51/42C07C51/48C07C51/47
Inventor 彭红云邢严张良张梦希
Owner ZHEJIANG UNIV
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