Preparation of pairing monoclonal antibody aiming to dairy cow Y sperm specificity and application thereof

Abstract

The invention discloses a preparation of pairing monoclonal antibody aiming to dairy cow Y sperm specificity and application thereof. Dairy cow spermatogonium is adopted to directly mithridatize a Balb/c mouse; after dairy cow spermatogonium is blended with Balb/c mice, tumor strain is built to screen; tumor cells are injected into the abdominal cavity of a mouse to produce hydroperitoneum; the hydroperitoneum of the mouse is extracted to be purified and screened to obtain pairing monoclonal antibody aiming to dairy cow Y sperm specificity; the pairing monoclonal antibody determines clusters respectively according to different antigens of H-Y antigen. The obtained pairing monoclonal antibody has high titer and strong specificity; testing proves that the pairing monoclonal antibody has no binding force to X sperm; based on principles of immunology, H-Y antibodies can be combined with Y sperm specificity to form an antigen-antibody compound; X sperm and Y sperm of the dairy cow can be separated by direct input method, IMB technology, centrifugal process, immune affinity and column chromatography so as to control the sex of dairy cows. The invention has the advantages of high efficiency, safety and low cost, is suitable for breeding stirps female on large scale and has higher economic benefits.

Description

[0001] The present invention relates to a method for sex control of dairy cows by separating X and Y sperm of cows, in particular to a method of separating X and Y sperm of cows by preparing specific monoclonal antibodies against Y sperm of cows, thereby realizing sex control of cows method. Background technique [0002] Dairy cows are single-born animals, and compared with other livestock species, their fecundity is low. Under natural conditions, the ratio of male to female in dairy cows is close to 1:1. The main purpose of raising dairy cows is to produce milk, so every breeder hopes that the cows will produce female calves to increase economic benefits. Increasing the proportion of cows through sex control technology can improve the reproductive efficiency of fine-bred dairy cows, thereby increasing the number of fine-bred dairy cows and improving the production level of dairy cows. It can be said that the realization of cow sex control is the wish of dairy farmers for man...

AI Technical Summary

Problems solved by technology

[0004] The early embryo sex identification method is to conduct sex identification on the pre-implantation embryo during embryo transfer, and artificially select embryos of a certain sex to transfer to the recipient, such as classic karyotype analysis, modern polymerase chain reaction (PCR), DNA probe method, etc., but these methods can only be applied to embryo transfer, and the survival rate of transplanted embryos is low, and it will also cause embryo waste. From the perspective of cost, this method is not suitable for large-scale breeding

[0005] Sperm separation by flow cytometry is based on the difference in DNA content between X and Y sperm. This gender control method has a high accuracy rate, but it has the disadvantages of slow separation speed, high technical cost, and some separation steps will affect the sperm quality. Damage and other disadvantages, not suitable for large-scale promotion and use