39 results about "Antibodies i" patented technology

The invention relates to a device for checking 10 food microbes synchronously and a screen diagnosis method, which adopts check test paper and a sample cup to process one-time synchronous check on 10 food microbes. The invention is characterized in that an immunity method is selected to select two matched monoclonal antibodies for different antigenic determinants of one strain as a food microbe antibody I and a food microbe antibody II; the invention utilizes prepared matched monoclonal antibodies and utilizes color particle immunity chromatography method to prepare test paper; the 10 food microbe test paper are arranged in one sample cup, and when adds a liquid sample, the one displays two read lines is considered as the existence of one microbe. The invention combines general food microbe agents together, to detect 10 microbes for one sample, thus being convenient for screening and on-time diagnosis.

The invention relates to the field of the emulsion method immune chromatography, and particularly relates to a detection kit of a helicobacter pylori emulsion method. The detection kit of the helicobacter pylori emulsion method comprises a reagent strip, wherein the reagent strip comprises a substrate, filter sample paper, a sensitized emulsion polyester film, an immune cellulose nitrate film and absorbent paper, wherein the filter sample paper, the sensitized emulsion polyester film, the immune cellulose nitrate film, and the absorbent paper are orderly connected end to end, and are fixed on the substrate, the immune cellulose nitrate film is covered with helicobacter pylori resisting urea enzyme antibodies I marked by colorful emulsion particles, and the immune cellulose nitrate film is provided with a detection line coated with helicobacter pylori resisting urea enzyme antibodies II and a quality control line coated by goat anti-rabbit serum IgG. The detection kit of the helicobacter pylori emulsion method, which is provided by the invention, has the advantages of convenience in detection, stability in detection results and cost conservation.

The invention discloses a surface enhanced Raman scattering immunochromatography test paper strip and a preparation method and application. The test paper strip comprises a bottom liner, a sample absorbing cushion, a combination cushion, a chromatography membrane and a water absorbing cushion. The sample absorbing cushion, the combination cushion, the chromatography membrane and the water absorbing cushion are closely connected in sequence and are attached to the bottom liner, wherein gold-kernel and silver-shell nanoflower marked antibodies I adhere to the combination cushion, a detection line is arranged on the chromatography membrane, and antibodies II or antigens A adhere to the detection line. According to the surface enhanced Raman scattering immunochromatography test paper strip and the preparation method and application, due to the fact that the gold-kernel and silver-shell nanoflower marked antibody I are dispersed on the combination cushion, the antigens A or the antibodies II linearly adhere to the chromatography membrane, and the detection line is formed; the sample absorbing cushion, the combination cushion, the chromatography membrane and the water absorbing cushion are sequentially connected to the bottom liner in a lap joint mode; the surface enhanced Raman scattering immunochromatography test paper strip is obtained. The surface enhanced Raman scattering immunochromatography test paper strip has the advantages of being safe to operate, easy and convenient to use, low in cost, fast to use, high in sensitivity and the like and is wide in application range.

The invention relates to an antigen detection method and application thereof. The method converts detection of an antigen signal into detection of a nucleic acid barcode label, and comprises the following operation steps: (1) labeling modified nano magnetic microspheres by using an antibody I of an antigen to be detected to prepare immune magnetic microspheres, and labeling nano-gold sol by using an antibody II of the antigen to be detected and a nucleic acid barcode fragment at the same time to prepare a double-labeled nano-gold probe; (2) fully combining the immune magnetic microspheres, the nano-gold probe and the antigen to be detected, removing combined aggregate, and leaving supernatant fluid for detection; and (3) detecting the nucleic acid barcode label in the supernatant fluid. The antigen detection method can be applied to the detection of clinical PSA antigen. The method has the advantages of simple operation, high sensitivity, good specificity, easy automation and wide application prospect.

The invention discloses a pathological specimen slice immunocytochemistry operation method and a pathological specimen slice immunocytochemistry operation system. The pathological specimen slice immunocytochemistry operation method comprises: slicing pathological specimen paraffin, baking the pathological specimen paraffin slice, removing the paraffin from the pathological specimen paraffin slice, carrying out antigen repair on the pathological specimen slice, staining the pathological specimen slice, adding antibodies to the pathological specimen slice in a dropwise manner, carrying out color developing on the pathological specimen slice, counterstaining the pathological specimen slice, sealing the pathological specimen slice, carrying out microscopic examination, and other steps. According to the present invention, staining is firstly performed with hematoxylin and then the antibodies I and II are added in the dropwise manner, such that the color developing of the antibodies can be easily achieved, the size and the position of the tissue can be easily grasped by sequentially carrying out the staining and the antibody adding in the dropwise manner, the position deviation during the antibody adding in the dropwise manner is reduced, the normative experiment operation can be easily performed, the strong practical value can be provided, the actual waste can be effectively avoided, and the error of the experiment data can be substantially reduced.

The present invention relates to the use of a novel, isolated anti-CXCR4 antibody in the diagnosis of cancer. In particular, methods for diagnosing and/or prognosing an oncogenic disorder associated with CXCR4 expression, are disclosed.

The invention discloses a test strip for rapidly detecting plasmodia based on colloidal gold immunochromatographic assay, as well as antibodies and cell lines thereof. The test strip comprises a basal plate, a sample pad, a polyester cellulose pad, a nitrocellulose membrane and a water absorbent pad, wherein the sample pad, the polyester cellulose pad, the nitrocellulose membrane and the water absorbent pad are partially superposed in sequence along the length direction of the basal plate and fixed on the basal plate; a colloidal gold-antibody I (H12) conjugate coating is sprayed on the polyester cellulose pad; a first test line, a second test line and a quality control line are distributed on the nitrocellulose membrane; the first test line is formed by performing spray-coating on an antibody II (A5) on the nitrocellulose membrane; the second test line is formed by performing spray-coating on an antibody IV (F6) on the nitrocellulose membrane; and the quality control line is formed by performing spray-coating on an goat anti-mouse IgG1 antibody on the nitrocellulose membrane. The test strip provided by the invention is easy to operate, rapid, sensitive and specific, and is applied to the rapid and early-stage diagnosis of malaria.

The invention relates to test paper for detecting chlamydia trachomatis infection, and the test paper comprises a baseplate, a sample pad, a conjugate pad, a nitrocellulose membrane and an absorption pad, wherein a detection line and a quality control line are arranged on the nitrocellulose membrane, the detection line on the nitrocellulose membrane is coated by mouse anti-human monoclonal protein antibody IgG (antibody II) of major outer membrane protein antigen of chlamydia trachomatis in the concentration of greater than or equal to 1mg/mL, and the quality control line is coated by goat anti-mouse IgG polyclonal antibody in the concentration of greater than or equal to 1.5mg/mL; the conjugate pad is coated by mouse anti-human monoclonal protein antibody IgG (antibody I) of the MOMP (major outer membrane protein) antigen of the chlamydia trachomatis, which is labeled by nano-gold and is in the concentration of not less than 6 mu g/mL; the sensitivity of detecting the MOMP of the chlamydia trachomatis of the test paper is 0.1ng/ml, the detection value range is 0-200ng/ml and the specificity is 98.5%; and the detection can be completed within 5 minutes.

A food-borne pathogenic bacteria quick detection method based on Gamma-Fe2O3@Au nano particle indirect enrichment and immunomagnetic separation, and belongs to the technical field of quick pathogen detection for food security. On dependence of a method for detecting pathogen in a food liquid sample, the specificity of an antibody I is combined with the target bacteria, the Gamma-Fe2O3@Au composite nano particle is utilized to prepare immuomagnetic beads of an antibody II to enrich target strains marked by the antibody I, the nano magnetic beads of the target bacteria are captured through separation, and then subjected to nitration to be converted into ferrous ions, and the ferrous ions are detected to indirectly detect whether the sample contains target pathogen. Under a certain condition, the quantity of the ferrous ions and the target bacteria show linear relation, so that the target bacteria can be quantificationally detected within a certain range. The food-borne pathogenic bacteria quick detection method can be used for quick detection of harmful pathogen in the food sample, and can be used for quick screening of large quantities of samples to be detected.

The invention relates to the field of animal medicine, and discloses a colloidal gold test strip capable of simultaneously detecting waterfowl source parvoviruses and a preparation method. The colloidal gold test strip comprises a base plate, a sample pad, a gold mark pad, a nitrocellulose membrane and an absorbent pad, the gold mark pad is coated with colloidal gold mark protein with gosling plague virus monoclonal antibodies I, detection lines and quality control lines are sequentially arranged on the nitrocellulose membrane along flowing directions of samples, the detection lines of the nitrocellulose membrane are coated with gosling plague virus monoclonal antibodies II, and the quality control lines of the nitrocellulose membrane are coated with goat-anti-mouse IgG (intravenous gamma globulin) antibodies. The colloidal gold test strip can simultaneously detect goose parvoviruses, Muscovy duck parvoviruses and novel Muscovy duck parvoviruses, false positive of reaction is reduced to a great extent, and specificity and sensitivity of detection are improved.

The invention provides a test strip and a method for detecting prostate tumor antigens. By the aid of the test strip and the method, the shortcomings of complicated operation and long time consuming of existing methods for detecting PSA (prostate specific antigens) and difficulty in meeting POC (point of care) quick diagnosis requirements can be overcome. PSA monoclonal antibodies II are labeled by gold nanorods by the aid of immunochromatography technologies, a nitrocellulose membrane is coated with goat anti-rat IgG and PSA monoclonal antibodies I, and the test strip for detecting the PSA isprepared by the aid of double-antibody sandwich processes. Liquid can flows through a binding pad and the nitrocellulose membrane step by step under capillary actions after PSA sample solution is added onto a sample pad of the test strip, and is bound with biological molecules, which are immobilized on the test strip, under antigen-antibody interaction, and a detection line visible to naked eyescan be generated. The test strip and the method are combined with microarray scanners, so that corresponding detection signal strength values can be obtained, and the PSA can be detected. The test strip and the method have the advantages that the method is simple and speedy, and is low in sample consumption and cost and short in detection time, the minimum concentration of the detectable PSA is 0.1 ng mL<-1>, the detection range is 0.1-100 ng mL<-1>, and clinical detection requirements can be met.

The invention discloses a novel tissue slice immunohistochemical operation method. The novel tissue slice immunohistochemical operation method includes the steps of 1), closing endogenous catalase and soaking by 0.3% methanol-hydrogen peroxide for 30 minutes; 2), staining by hematoxylin for 2 minutes; 3), dropwise adding 40 microlitres of antibody I and standing at room temperature for 40 minutes-1 hour; 4), dropwise adding 40-50 microlitres of antibody II and standing at room temperature or 37 DEG C for 1 hour. The novel tissue slice immunohistochemical operation method has the advantages that a conventional operation step that the antibody I and the antibody II are stained by the hematoxylin before being dropwise added is changed to be beneficial to developing of the antibody I and the antibody II which are dropwise added. The novel tissue slice immunohistochemical operation method is simple in operation step and succeeds in changing existing operation effects only by making changes on operation sequences of existing operation steps, adopted reagents and operation time, and the antibodies are added dropwise after staining to facilitate confirmation of sizes and positions of tissues.

A food-borne pathogenic bacteria quick detection method based on Fe3O4 nano particle indirect enrichment and immunomagnetic separation belongs to the technical field of quick pathogen detection for food security. On dependence of a method for detecting pathogen in a food liquid sample, the specificity of an antibody I is combined with the target bacteria, the Fe3O4 nano particle material is utilized to prepare immuomagnetic beads of an antibody II to enrich target strains marked by the antibody I, the nano magnetic beads of the target bacteria are captured through separation, and then subjected to nitration to be converted into ferrous ions, and the ferrous ions are detected to indirectly detect whether the sample contains target pathogen. Under a certain condition, the quantity of the ferrous ions and the target bacteria show linear relation, so that the target bacteria can be quantificationally detected within a certain range. The food-borne pathogenic bacteria quick detection method can be used for quick detection of harmful pathogen in the food sample, and can be used for quick screening of large quantities of samples to be detected.

The invention discloses a quantitative immune colloidal gold detection card for urine cystatin C, urine microalbumin and urine creatinine. The detection card comprises a buckle box, a bottom plate, and a sample pad, a combination pad, a chromatographic membrane and a sample absorption pad which are sequentially adhered to the bottom plate. The combination pad is coated with a colloidal gold labeled cystatin C antibody I, a urine microalbumin antibody I, a urine creatinine antibody I and a chicken IgY. A first detection line coated with a cystatin C antibody II, a second detection line coated with a urine microalbumin antibody II, a third detection line coated with a urine creatinine antigen modified by BSA, and a quality control line coated with goat anti-chicken IgY are arranged on the chromatographic membrane. Compared with single detection of U-mALb and U-Cr values, the U-mAlb/U-Cr ratio obtained in the invention can more sensitively reflect the degree of renal function impairment,and the accuracy of the detection result is improved.

The invention discloses a quantitative detection kit for Sflt-1. The kit comprises a substrate and a cover plate, the substrate and the cover plate are formed by bonding, a sample adding hole and a detection hole are formed in the cover plate, a sample adding area, a reaction area, a detection area and a waste liquid area are sequentially arranged on the substrate, the reaction area is coated witha fluorescent microsphere labeled sFLT-1 antibody I, and the detection area is internally and fixedly coated with sFLT-1 antibodies II with different epitopes. The kit is high in sensitivity, good inrepeatability, extremely small in required sample size, short in detection time and simple and convenient to operate, the operation process can be greatly simplified, the consumption of samples and reagents is reduced, expensive instruments do not need to be arranged, on-site instant detection becomes possible, and the kit has huge economic value and social value; meanwhile, a doctor can be helped to diagnose related diseases of preeclampsia, and early identification and intervention are performed on high-risk groups, so that the safety of maternal and infant in the gestation period is guaranteed.

The present invention relates to clinical blood detecting and analyzing technology, and is the cTnI Luminol chemiluminescence immunological analysis detecting method with the system including self made cardiac muscle calcium protein resisting antibody I(cTnI) to label horseradish peroxidase, cinnamic acid as luminescence intensifier, tetreaphenyl sodium borate as synergistic intensifier and horseradish peroxidase as catalyst for Luminol-H2O2 luminescence. Compared with available ELISA process, the said CLIA process of the present invention has no obvious difference, correlation coefficient of 0.9852 and diagnosis coincidence rate of 96.15 %.

The invention belongs to the technical field of assay, and particularly relates to a kit for rapidly and quantitatively assaying PD-L1 (programmed death ligand-1) by chemiluminescent magnetic bead enzyme-linked immunosorbent assay. The kit for rapidly and quantitatively assaying PD-L1 by chemiluminescent magnetic bead enzyme-linked immunosorbent assay disclosed by the invention consists of standards, mouse anti-human PD-L1 antibody I-coated immunomagnetic beads, an enzyme-labeled mouse anti-human PD-L1 antibody II, chemiluminescent substrates, and auxiliary reagents. The kit for rapidly and quantitatively assaying PD-L1 by chemiluminescent magnetic bead enzyme-linked immunosorbent assay disclosed by the invention has the advantages of high specificity, good stability, high sensitivity andlower cost, and is suitable for large-scale clinic popularization.

The invention relates to an NT-proBNP quantitative detection kit and a preparation method thereof, the NT-proBNP quantitative detection kit comprises a chip and a reagent, and the chip comprises a chip substrate and a chip cover plate; a sample adding area, a reaction area, a detection area and a waste liquid pool are sequentially arranged on the chip substrate; the sample adding area is communicated with the reaction area through a filtering channel, and a blood filtering film is arranged in the filtering channel; the reaction area is communicated with the detection area through a reaction channel, a first flow limiting column is arranged in the reaction channel, the first flow limiting column is of a bent structure, and the bending direction extends in the width direction of the reaction channel; the detection area is communicated with the waste liquid pool through a waste liquid channel, a second flow limiting column is arranged at the communication position of the waste liquid channel and the waste liquid pool and is of a wave-shaped structure, and a protrusion is arranged at the end, facing the waste liquid pool, of the second flow limiting column; the reaction area is coated with an NT-proBNP antibody I marked by fluorescent microspheres, and an NT-proBNP antibody II is fixed in the detection area; a sample adding hole, a detection window and an air hole are formed in the chip cover plate. The kit has the advantages of rapidness, simplicity and convenience in detection and capability of greatly improving the detection sensitivity and accuracy.

The invention relates to the field of latex process immunochromatography, and particularly discloses a plasmodium falciparum latex process detection kit. The kit comprises a reagent strip, wherein the reagent strip comprises a substrate, filtering sample paper, a sensitized latex polyester membrane, an immune nitrocellulose membrane and water absorption paper, wherein the filtering sample paper, the sensitized latex polyester membrane, the immune nitrocellulose membrane and the water absorption paper are sequentially in end-to-end connection and are fixed on the substrate; color latex particle-marked plasmodium falciparum-resistant histidine enriched protein II monoclonal antibodies I cover the sensitized latex polyester membrane; detection lines coated by plasmodium falciparum-resistant histidine enriched protein II monoclonal antibodies II and quality control lines coated with goat anti-rabbit IgG are arranged on the immune nitrocellulose membrane. The plasmodium falciparum latex process detection kit has the advantages that the response is sensitive; the detection is convenient; the detection result is stable; the cost is reduced.