Immunochromatographic test strip and preparation method thereof

A technology of immunochromatography test strips and quality control substances, which is applied in the direction of analytical materials, instruments, and measuring devices, which can solve problems such as small detection ranges, achieve good test precision, and improve test accuracy.

Active Publication Date: 2020-06-23
东莞市东阳光诊断产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the C line signal is almost unchanged, the T / C value is only changed by the increase of the T value signal, so the detection range is small

Method used

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  • Immunochromatographic test strip and preparation method thereof
  • Immunochromatographic test strip and preparation method thereof
  • Immunochromatographic test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] This embodiment 1 provides an alpha-fetoprotein AFP fluorescent immunochromatographic test strip, the binding pad is coated with two kinds of markers, wherein the second marker is labeled with mouse monoclonal antibody I and rabbit IgG antibody, correspondingly, C The line is coated with goat anti-rabbit IgG antibody, and the specific preparation method is as follows:

[0079] (1) Reagents and instruments

[0080] Proclin 300: Aladdin

[0081] PVP: Aladdin

[0082] Trehalose: Aladdin

[0083] Sucrose: Chengdu Kelon Chemical Co., Ltd.

[0084] BSA: Roche

[0085] Europium ion fluorescent microspheres (particle size 300nm, surface functional groups are carboxyl groups): Bangs Labs

[0086] Alpha-fetoprotein AFP antibody: Medix(5108)

[0087] Alpha-fetoprotein AFP antibody: Artron (AFP-A51-Ab1)

[0088] Goat anti-mouse antibody: Luoyang Baitaike Biotechnology Co., Ltd.

[0089] Goat anti-rabbit IgG antibody: Hangzhou Xianzhi Biotechnology

[0090] Rabbit IgG antib...

Embodiment 2

[0123] This embodiment 2 provides an alpha-fetoprotein AFP fluorescent immunochromatographic test strip, the binding pad is coated with two markers, wherein the second marker is labeled with mouse monoclonal antibody I and chicken IgY antibody, correspondingly, C Line-coated sheep anti-chicken IgY antibody, the specific preparation method is as follows:

[0124] (1) Reagents and instruments

[0125] Europium ion fluorescent microspheres (particle size 200nm, surface functional groups are carboxyl groups): Bangs Labs

[0126] Goat Anti-Chicken IgY and Chicken IgY: Boyin Bio

[0127] All the other reagent raw materials and equipment are with embodiment 1

[0128] (2) Preparation of alpha-fetoprotein AFP fluorescent immunochromatographic test strips

[0129] (1) NC film preparation

[0130] (1.1) Paste and fix the NC film on the middle part of the PVC bottom plate;

[0131] (1.2) Dilute AFP mouse monoclonal antibody II (Medix 5108) to 1.5mg / ml and goat anti-chicken IgY antib...

Embodiment 3

[0152] This embodiment 3 provides a total prostate-specific antigen TPSA fluorescent immunochromatographic test strip, the binding pad is coated with two kinds of markers, wherein the second marker is labeled with TPSA mouse monoclonal antibody I and biotinylated TPSA mouse Monoclonal antibody I, correspondingly, the C line is coated with streptavidin, and the specific preparation method is as follows:

[0153] (1) Reagents and instruments

[0154] Europium ion fluorescent microspheres (particle size 200nm, surface functional groups are carboxyl groups): Bangs Labs

[0155] Total Prostate Specific Antibody TPSA Antibody: Cytocon 1006

[0156] Total Prostate Specific Antibody TPSA Antibody: Cytocon 1005

[0157] Biotin: Thermo

[0158] Streptavidin: Roche

[0159] All the other reagent raw materials and equipment are with embodiment 1

[0160] (2) Preparation of total prostate-specific antigen TPSA fluorescent immunochromatographic test strips

[0161] (1) NC film prepara...

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Abstract

The invention relates to an immunochromatographic test strip and a preparation method thereof. The immunochromatographic test strip comprises a sample pad, a combination pad and a reaction film whichare arranged on a substrate, the combination pad is coated with a first marker and a second marker; wherein the first marker is a compound in which a target antibody I is coupled to a labeled microsphere I, the second marker is a compound in which the target antibody I and a quality control substance I are jointly coupled to a labeled microsphere II, the detection line is coated with a target antibody II, and the quality control line is coated with a quality control substance II capable of being specifically combined with the quality control substance I. The test strip provided by the invention adopts a new quality control system, not only eliminates the interference of a T-line antibody to a C line and the interference of a common blocking agent and a common interfering substance, but also changes the signals of the T line and the C line along with the change of the concentration of a target object, effectively improves the detection precision, and widens the linear detection range.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to an immunochromatography test strip and a preparation method thereof. Background technique [0002] Immunochromatography is an immunological analysis method that appeared in the early 1980s. Usually, the strip-shaped fiber chromatography material is used as the solid phase, and the sample solution swims on the chromatography strip through capillary action, and the immune reaction through the combination of antigen and antibody The principle is that during the chromatography process, the immune complex is enriched or trapped in a certain area (detection line) of the chromatography material to obtain intuitive experimental results through enzyme reactions or detectable markers. [0003] With the development of immunochromatography technology, there are now immunochromatographic test strips that can be used for quantitative detection. Taking the chromatographi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/558G01N33/54313
Inventor 郑兰花熊亮洪礼清蒋奎胜陈郑刘仁源
Owner 东莞市东阳光诊断产品有限公司
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