NT-proBNP quantitative detection kit and preparation method thereof

Abstract

The invention relates to an NT-proBNP quantitative detection kit and a preparation method thereof, the NT-proBNP quantitative detection kit comprises a chip and a reagent, and the chip comprises a chip substrate and a chip cover plate; a sample adding area, a reaction area, a detection area and a waste liquid pool are sequentially arranged on the chip substrate; the sample adding area is communicated with the reaction area through a filtering channel, and a blood filtering film is arranged in the filtering channel; the reaction area is communicated with the detection area through a reaction channel, a first flow limiting column is arranged in the reaction channel, the first flow limiting column is of a bent structure, and the bending direction extends in the width direction of the reaction channel; the detection area is communicated with the waste liquid pool through a waste liquid channel, a second flow limiting column is arranged at the communication position of the waste liquid channel and the waste liquid pool and is of a wave-shaped structure, and a protrusion is arranged at the end, facing the waste liquid pool, of the second flow limiting column; the reaction area is coated with an NT-proBNP antibody I marked by fluorescent microspheres, and an NT-proBNP antibody II is fixed in the detection area; a sample adding hole, a detection window and an air hole are formed in the chip cover plate. The kit has the advantages of rapidness, simplicity and convenience in detection and capability of greatly improving the detection sensitivity and accuracy.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to an NT-proBNP (N-terminal brain natriuretic peptide precursor) quantitative detection kit and a preparation method thereof. Background technique [0002] B-type natriuretic peptide (B-type natriuretic peptide) is mainly derived from the ventricle, and is a good serum marker for judging the severity and prognosis of chronic heart failure. Under normal circumstances, only a small amount of BNP is stored in cardiomyocytes, and its secretion depends on the activation of the BNP gene. When clinically relevant factors such as atrial fibrillation, valvular heart disease, increased ventricular volume load, pulmonary hypertension, changes in the size and function of the left and right ventricles, coronary heart disease and other clinically relevant factors lead to ventricular muscle stretch or ischemia, the BNP gene of cardiomyocytes is activated, and the BNP gene ...

AI Technical Summary

Problems solved by technology

Immunochemiluminescence method is a new method improved on the basis of radioimmunoassay and enzyme immunoassay. It has no radioactive and teratogenic substances. It has the advantages of the above two methods, and the product has a long validity period and is environmentally friendly. Pollution, non-toxic and harmless to the human body, it is the best in vitro diagnostic reagent detection method at present. It has obvious advantages in technology, but the disadvantage is also cumbersome operation, long time-consuming, and high cost, and is not suitable for rapid bedside detection.

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