Surface enhanced Raman scattering immunochromatography test paper strip and preparation method and application

Abstract

The invention discloses a surface enhanced Raman scattering immunochromatography test paper strip and a preparation method and application. The test paper strip comprises a bottom liner, a sample absorbing cushion, a combination cushion, a chromatography membrane and a water absorbing cushion. The sample absorbing cushion, the combination cushion, the chromatography membrane and the water absorbing cushion are closely connected in sequence and are attached to the bottom liner, wherein gold-kernel and silver-shell nanoflower marked antibodies I adhere to the combination cushion, a detection line is arranged on the chromatography membrane, and antibodies II or antigens A adhere to the detection line. According to the surface enhanced Raman scattering immunochromatography test paper strip and the preparation method and application, due to the fact that the gold-kernel and silver-shell nanoflower marked antibody I are dispersed on the combination cushion, the antigens A or the antibodies II linearly adhere to the chromatography membrane, and the detection line is formed; the sample absorbing cushion, the combination cushion, the chromatography membrane and the water absorbing cushion are sequentially connected to the bottom liner in a lap joint mode; the surface enhanced Raman scattering immunochromatography test paper strip is obtained. The surface enhanced Raman scattering immunochromatography test paper strip has the advantages of being safe to operate, easy and convenient to use, low in cost, fast to use, high in sensitivity and the like and is wide in application range.

Description

technical field [0001] The invention belongs to the field of spectrum and immunological detection and detection, and in particular relates to a surface-enhanced Raman scattering immunochromatographic test strip, a preparation method and application thereof. Background technique [0002] Immunological detection methods are a series of experimental methods designed to detect antigens, antibodies, immune cells and their secreted cytokines and small molecules by applying immunological theory. In immunological detection, radioimmunoassay, enzyme-linked immunosorbent assay (ELISA), electrochemical immunoassay technology, electrochemiluminescence immunoassay technology, colloidal gold immunochromatography and other methods are mainly used at home and abroad to achieve quantitative or qualitative detection . But these methods all have different degrees of disadvantages: [0003] ①Enzyme-linked immunosorbent assay has high sensitivity, but requires some supporting professional inst...

AI Technical Summary

Problems solved by technology

[0003] ①Enzyme-linked immunosorbent assay has high sensitivity, but requires some supporting professional instruments and equipment, professional personnel to operate, certain requirements for the detection environment, long detection time, and cumbersome steps

The signal amplification ability of a single fluorescent dye is limited, and fluorescence quenching is prone to occur under light conditions, which affects the sensitivity and stability of the test strip

Quantum dots have the characteristics of wide excitation spectrum, narrow emission spectrum, large Stokes shift and high stability, but quantum dots also have some shortcomings: quantum dots have medium brightness and high price, which affect the sensitivity of test strips and increase The cost of development and production of test strips

However, fluorescent latex microspheres, like organic fluorescent dyes, have a small Stokes shift and are easily disturbed by background light, which affects their sensitivity.

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