Pathological specimen slice immunocytochemistry operation method and pathological specimen slice immunocytochemistry operation system

A technology of immunohistochemistry and operation method, applied in the field of immunohistochemistry operation method of pathological specimen slice and its system, can solve the problems of inaccurate experimental data, low efficiency of disease work, inaccurate grasp, etc., so as to reduce errors and avoid The effect of wasting and reducing the deviation of grasp

Pending Publication Date: 2017-11-21
CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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Problems solved by technology

[0005] The present invention solves the problems in the prior art that the operators in the immunohistochemical experiment do not accurately grasp...

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  • Pathological specimen slice immunocytochemistry operation method and pathological specimen slice immunocytochemistry operation system

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Embodiment 1

[0038] A method for immunohistochemical operation of pathological specimen slices, comprising the following steps:

[0039] (1) Paraffin sections of pathological specimens: take the prepared paraffin of pathological specimens, and cut them into 3 μm paraffin sections with a paraffin microtome;

[0040] (2) Baking slices of pathological specimens: Bake the glass slides with paraffin sections of pathological specimens in a constant temperature oven at 60°C for 2 hours, and dry the paraffin sections of pathological specimens;

[0041] (3) Dewaxing of pathological specimen sections: Before dewaxing, place the glass slides with paraffin sections of pathological specimens at room temperature for 60 minutes, let them cool down, and return to the same temperature as room temperature, and then use xylene Ⅰ and xylene Ⅱ , xylene III for 5 minutes each time, then place the slides in absolute alcohol I, absolute alcohol II, and absolute alcohol III for 2 minutes each time, and then move t...

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Abstract

The invention discloses a pathological specimen slice immunocytochemistry operation method and a pathological specimen slice immunocytochemistry operation system. The pathological specimen slice immunocytochemistry operation method comprises: slicing pathological specimen paraffin, baking the pathological specimen paraffin slice, removing the paraffin from the pathological specimen paraffin slice, carrying out antigen repair on the pathological specimen slice, staining the pathological specimen slice, adding antibodies to the pathological specimen slice in a dropwise manner, carrying out color developing on the pathological specimen slice, counterstaining the pathological specimen slice, sealing the pathological specimen slice, carrying out microscopic examination, and other steps. According to the present invention, staining is firstly performed with hematoxylin and then the antibodies I and II are added in the dropwise manner, such that the color developing of the antibodies can be easily achieved, the size and the position of the tissue can be easily grasped by sequentially carrying out the staining and the antibody adding in the dropwise manner, the position deviation during the antibody adding in the dropwise manner is reduced, the normative experiment operation can be easily performed, the strong practical value can be provided, the actual waste can be effectively avoided, and the error of the experiment data can be substantially reduced.

Description

technical field [0001] The invention belongs to the technical field of laboratory detection and analysis methods, and in particular relates to an immunohistochemical operation method and system for pathological specimen slices. Background technique [0002] Immunohistochemistry is the application of the principle of immunological antigen-antibody reaction, that is, the principle of specific reaction between antigen and antibody. Through chemical reaction, the chromogenic agent of the labeled antibody, such as fluorescein, enzyme, metal ion, isotope, etc., is developed to determine the reaction tissue cells. Antigen, its localization, qualitative and quantitative research. [0003] For immunohistochemical operations, experimenters often encounter tissue sections that have no color after repairing. When adding I antibody and II antibody, they do not know the specific dripping location and tissue, so they can only take a large area. Dropping, resulting in inaccurate dropping r...

Claims

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Application Information

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IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 姜美容周梅华彭靖黎娅商亚彤
Owner CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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