Pathological specimen slice immunocytochemistry operation method and pathological specimen slice immunocytochemistry operation system

Abstract

The invention discloses a pathological specimen slice immunocytochemistry operation method and a pathological specimen slice immunocytochemistry operation system. The pathological specimen slice immunocytochemistry operation method comprises: slicing pathological specimen paraffin, baking the pathological specimen paraffin slice, removing the paraffin from the pathological specimen paraffin slice, carrying out antigen repair on the pathological specimen slice, staining the pathological specimen slice, adding antibodies to the pathological specimen slice in a dropwise manner, carrying out color developing on the pathological specimen slice, counterstaining the pathological specimen slice, sealing the pathological specimen slice, carrying out microscopic examination, and other steps. According to the present invention, staining is firstly performed with hematoxylin and then the antibodies I and II are added in the dropwise manner, such that the color developing of the antibodies can be easily achieved, the size and the position of the tissue can be easily grasped by sequentially carrying out the staining and the antibody adding in the dropwise manner, the position deviation during the antibody adding in the dropwise manner is reduced, the normative experiment operation can be easily performed, the strong practical value can be provided, the actual waste can be effectively avoided, and the error of the experiment data can be substantially reduced.

Description

technical field [0001] The invention belongs to the technical field of laboratory detection and analysis methods, and in particular relates to an immunohistochemical operation method and system for pathological specimen slices. Background technique [0002] Immunohistochemistry is the application of the principle of immunological antigen-antibody reaction, that is, the principle of specific reaction between antigen and antibody. Through chemical reaction, the chromogenic agent of the labeled antibody, such as fluorescein, enzyme, metal ion, isotope, etc., is developed to determine the reaction tissue cells. Antigen, its localization, qualitative and quantitative research. [0003] For immunohistochemical operations, experimenters often encounter tissue sections that have no color after repairing. When adding I antibody and II antibody, they do not know the specific dripping location and tissue, so they can only take a large area. Dropping, resulting in inaccurate dropping r...

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Problems solved by technology

[0005] The present invention solves the problems in the prior art that the operators in the immunohistochemical experiment do not accurately grasp the position when adding the antibody, the disease work efficiency is low, the waste of solvent is large, and the experimental data is inaccurate, etc.

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