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Quantitative immune colloidal gold detection card and kit for urocystatin C, urine microalbumin and urine creatinine

A technology of urinary microalbumin and colloidal gold, which is applied in the field of colloidal gold immunochromatography, can solve the problems of complex operation of the detection method, long detection time, high detection cost, etc., and achieve improved detection rate and detection accuracy, simple operation, The effect of simplifying inspection operations

Pending Publication Date: 2020-04-10
HUNAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing products on the market are detection kits for a single indicator, and only a small number of products can detect U-mALb, U-Cr, and U-mALb / Cr ratio at the same time, and the related detection methods are complicated to operate and expensive to detect. high
[0006] The Chinese invention patent with the publication number CN108088839B discloses a urine microalbumin / urine creatinine detection kit, which uses chemiluminescence to detect urine microalbumin and urine creatinine; Incubate with the sample by shaking, the operation is complicated, and the detection time is long

Method used

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  • Quantitative immune colloidal gold detection card and kit for urocystatin C, urine microalbumin and urine creatinine
  • Quantitative immune colloidal gold detection card and kit for urocystatin C, urine microalbumin and urine creatinine
  • Quantitative immune colloidal gold detection card and kit for urocystatin C, urine microalbumin and urine creatinine

Examples

Experimental program
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Effect test

Embodiment 1

[0050] like Figure 1~2 As shown, this embodiment provides a quantitative immune colloidal gold detection card for urinary cystatin C, urinary microalbumin and urine creatinine, including a snap box, a base plate, and a sample pad, a binding pad, and a chromatography pad sequentially pasted on the base plate. Membrane and sample suction pad, the bottom plate is installed in the buckle box; wherein, the bottom plate is PVC board, the chromatography membrane is nitrocellulose membrane, the sample suction pad is water-absorbing filter paper, and the sample pad and binding pad are made of glass fiber.

[0051] In this example, colloidal gold-labeled cystatin C antibody I, urine microalbumin antibody I, urine creatinine antibody I and chicken IgY are coated on the binding pad, and the chromatographic membrane is coated with cystatin C antibody II The first detection line, the second detection line coated with urinary microalbumin antibody II, the third detection line coated with BS...

Embodiment 2

[0059] The present embodiment provides a preparation method of urinary cystatin C, urine microalbumin and urine creatinine quantitative immune colloidal gold detection card, specifically comprising the following steps:

[0060] S1. Preparation of colloidal gold labeled antibody application solution: first prepare colloidal gold solution, put 1L ultrapure water in the reactor, add chloroauric acid to it, add trisodium citrate after boiling, continue to boil for 10 minutes, chloroauric acid is finally The concentration is 0.04%, and the final concentration of trisodium citrate is 0.01%. After cooling, dilute to 1L with ultrapure water, collect the product, and the diameter of the colloidal gold solution is 40mm;

[0061] Take 5 mL of colloidal gold solution, under slow stirring, adjust the pH value of the solution to 7.5 with 0.2 M potassium carbonate buffer solution, then add 30 μg Cystatin C antibody I, continue stirring for 30 min, add BSA with a final concentration of 0.5%, ...

Embodiment 3

[0073] The present embodiment provides a preparation method of urinary cystatin C, urine microalbumin and urine creatinine quantitative immune colloidal gold detection card, specifically comprising the following steps:

[0074] S1. Preparation of colloidal gold labeled antibody application solution: first prepare colloidal gold solution, put 1L ultrapure water in the reactor, add chloroauric acid to it, add trisodium citrate after boiling, continue to boil for 10 minutes, chloroauric acid is finally The concentration is 0.05%, and the final concentration of trisodium citrate is 0.02%. After cooling, dilute to 1L with ultrapure water, collect the product, and the diameter of the colloidal gold solution is 45mm;

[0075] Take 5 mL of colloidal gold solution, under slow stirring, adjust the pH value of the solution to 7.5 with 0.2 M potassium carbonate buffer solution, then add 20 μg Cystatin C antibody I, continue stirring for 30 min, add BSA with a final concentration of 0.5%, ...

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Abstract

The invention discloses a quantitative immune colloidal gold detection card for urine cystatin C, urine microalbumin and urine creatinine. The detection card comprises a buckle box, a bottom plate, and a sample pad, a combination pad, a chromatographic membrane and a sample absorption pad which are sequentially adhered to the bottom plate. The combination pad is coated with a colloidal gold labeled cystatin C antibody I, a urine microalbumin antibody I, a urine creatinine antibody I and a chicken IgY. A first detection line coated with a cystatin C antibody II, a second detection line coated with a urine microalbumin antibody II, a third detection line coated with a urine creatinine antigen modified by BSA, and a quality control line coated with goat anti-chicken IgY are arranged on the chromatographic membrane. Compared with single detection of U-mALb and U-Cr values, the U-mAlb / U-Cr ratio obtained in the invention can more sensitively reflect the degree of renal function impairment,and the accuracy of the detection result is improved.

Description

technical field [0001] The invention relates to the technical field of colloidal gold immunochromatography, and more specifically relates to a quantitative immunocolloidal gold detection card and kit for urinary cystatin C, urinary microalbumin and urinary creatinine. Background technique [0002] Chronic kidney disease is a common high-incidence disease in my country, with an adult incidence rate of 10.8%. If chronic kidney disease cannot be effectively controlled, it will progress to end-stage renal disease. At this time, patients need to rely on dialysis or kidney transplantation to maintain their lives. Therefore, the early screening and diagnosis of chronic kidney disease, timely intervention and treatment of chronic kidney disease, and the reversal of impaired renal function are of great significance to the prevention and treatment of chronic kidney disease. [0003] Cystatin C (CystatinC, CysC) is an extracellular inhibitor of cysteine ​​protease, belongs to the type ...

Claims

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Application Information

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IPC IPC(8): G01N33/70G01N33/68G01N33/58G01N33/558
CPCG01N33/70G01N33/6893G01N33/585G01N33/558G01N2333/8139G01N2333/76G01N2800/347
Inventor 黄钊贾学恩汤建新
Owner HUNAN UNIV OF TECH
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