This invention discloses an electrochemical sensor for detecting Cys C, its preparation method, and a method for detecting Cys C. The electrochemical sensor for detecting Cys C includes a
working electrode, a
reference electrode, and a counter
electrode. The
working electrode comprises an
electrode substrate and
nanomaterials and a Cys C
aptamer sequentially modified on the
electrode substrate. The
nanomaterials are MoS2
nanomaterials or gold nanomaterials. The 5′ end of the Cys C
aptamer is modified with an electroactive substance X, where X is
ferrocene or
methylene blue. The 3′ end of the Cys C
aptamer is modified with a group Y, where Y is -(CH2). n -SH, where n is a natural number from 1 to 12. The electrochemical sensor of this invention can be used for
in vivo quantitative detection of Cys C, and exhibits good performance in terms of cost-effectiveness, ease of operation, and the use of
environmentally friendly materials. It is a potential electrochemical sensor for early diagnosis of CKD.