60results about How to "Expand the scope of the filter" patented technology

The invention relates to technology for screening and arranging winter plants in artificial wetlands for purifying domestic sewage in subtropical regions. A method comprises the following steps of: screening seven plants with high purification capabilities, such as cress, rape, juncus effuses, saxifrage, Iris germannica, sweet osmanthus flower and flagIris according to adaptation capabilities andthree indexes such as a physiological property, a pollution resisting and removing capability and matrix enzyme activity; and combining and arranging the screened plants according to a sewage purification effect and a landscape effect. A winter artificial wetland plant mode has a good sewage purification effect, can operate safely and effectively in the subtropical regions and has certain landscape value.

The invention relates to the field of protein engineering, in particular to a method for preparing CysC-paired monoclonal antibodies, which comprises the following steps of: screening hybridoma cells having specific binding with CysC after fusing mouse splenic cells and myeloma cells capable of generating CysC antibodies, preparing cells for generating the monoclonal antibodies, finishing first-time cell fusion, further purifying out the monoclonal antibodies, and selecting one monoclonal antibody to be marked with horse radish peroxidase; then implementing the second-time fusion of the mouse splenic cells and the myeloma cells; finishing CysC-antigen coating on an elisa plate, adding a hybridoma-cell cultural supernatant obtained through the second-time cell fusion to elisa-plate holes, arranging a blank control hole, subsequently adding the monoclonal antibody marked with the horse radish peroxidase to the elisa-plate holes, incubating at the temperature of 37 DEG C for 30 minutes, adding a substrate to each hole for color rendering, and measuring a light absorption value under 450nm after stopping reaction through sulfuric acid; and selecting the uninhibited holes as the paired monoclonal antibodies.

The invention provides a method for preparing a poly(4-methyl-1-pentene) hollow fiber membrane with a gradient pore structure through coupling of a thermally induced phase separation method and a non-solvent induced phase separation method. The method comprises the following steps: uniformly mixing poly(4-methyl-1-pentene) and a diluent at high temperature, and carrying out extrusion to realize one-shot molding; after the formed mixture passes through an air section, allowing the mixture to enter a cooling bath for cooling so as to realize thermally induced phase separation and non-solvent phase separation; and finally, extracting the diluent to obtain the hollow fiber membrane. The hollow fiber membrane prepared by the method provided by the invention has improved safety, and the thermally induced phase separation process and the non-solvent induced phase separation process in a poly(4-methyl-1-pentene) diluent system are easy to regulate and control, so the poly(4-methyl-1-pentene) membrane with better mechanical strength, gas permeability and plasma infiltration resistance is obtained. The invention also provides the poly(4-methyl-1-pentene) hollow fiber membrane and applicationthereof in the field of artificial membrane lungs.

The invention discloses a river sand screening device for construction. The sand screening device comprises a sand feeding hopper, a drying cylinder, a primary screening device and a secondary sand screening device, the drying cylinder is mounted at the upper end of a bracket; the drying cylinder is of a double-layer hollow structure, a heating block is arranged between the outer cylinder wall andthe inner cylinder wall of the drying cylinder; one end of the drying cylinder is connected with a sand inlet hopper; the other end of the drying cylinder is communicated with the primary screening device; the primary screening device is a sand screening barrel; the sand screening barrel is installed on a supporting frame, first screening holes are formed in the side wall of the sand screening barrel, the second-stage sand screening device comprises a swing frame and a sand filtering plate, a sand screening plate is fixed to the upper end of a swing frame, second screening holes are formed inthe sand screening plate, the swing frame is connected with the supporting frame and located below the sand screening barrel, and the sand screening plate and the sand screening barrel are opposite in inclination direction. The problem that wet sands with small particles are mutually adhered is solved, the working efficiency is improved, the screening effect is improved, the utilization rate of the sand is increased, waste is avoided, and the construction cost is reduced.

The invention belongs to the technical field of biological environment protection, and in particular relates to a plant screening and configuring method for improving an operation effect of a subsurface flow type constructed wetland in winter. Ligneous plants and herbaceous plants are screened out under the comprehensive consideration of the characteristics such as stain resistance and purification ability, growth property, rhizosphere enzyme activity, landscape property of the plants, and the grown plants are periodically maintained and managed. According to the method, a good operation effect of the constructed wetlands in winter is guaranteed, the landscape property of four seasons in the overall year is maintained, the promotion and application bottleneck of the wetlands is effectively solved, and the application prospect is wide.

The invention discloses a preparation method of a pepsinogen I paired monoclonal antibody. The preparation method of the pepsinogen I paired monoclonal antibody comprises the following steps: (1) immunizing BALB / c mice with pepsinogen I protein as an antigen, and selecting immunized mice with high serum antibody titer for cell fusion, screening a high-titer positive cell line, preparing ascites, and purifying to obtain a monoclonal antibody; and (2) mixing the pepsinogen I protein with the monoclonal antibody obtained in the step (1) to prepare antigen-monoclonal antibody immune complex immunized BALB / c mice, selecting mice with high serum antibody titer for cell fusion, screening a positive cell line having no competitive inhibition effect with the monoclonal antibody of the step (1), andpurifying the secreted monoclonal antibody to obtain a monoclonal antibody paired with the monoclonal antibody of the step (1). The method effectively improves the probability of obtaining the pairedmonoclonal antibody of the pepsinogen I antigen.

The invention discloses a microfluidic-based high-flux crystallization condition screening method. The method comprises the following steps of S1, pouring a mixture of PDMS and a cross-linking agent into a cleaned mold, vacuumizing, heating for curing, stripping the cured PDMS from the mold, pasting the PDMS to a substrate, and heating for curing to obtain a microfluidic chip; S2, uniformly mixinga substance to be crystallized, a solvent and an anti-solvent to output a dispersed phase, pouring a continuous phase and the dispersed phase into a channel of the microfluidic chip, and generating liquid drops containing crystals under several crystallization conditions; S3, collecting the liquid drops containing the crystals; and S4, identifying a crystal morphology, and screening a corresponding crystallization condition according to the crystal morphology. The microfluidic chip can be used as a micro-reactor, and has advantages of high-flux treatment, rapid reaction, low reagent consumption, flexible operation, automation, integration and the like.

The invention provides patent compilation and analysis application software which comprises a carrier client, a central processing unit, a cloud platform and a software server cluster, and the carrierclient is a computer provided with the software and is in telecommunication connection with the central processing unit. By arranging the central processing unit with a plurality of modules, communication, compilation, analysis and data transmission can be achieved, and diversification of software functions is achieved. The system is also provided with a software server cluster comprising different types of servers. According to the method and the system, the user access and file storage can be efficiently provided, the system configuration and management are facilitated, the data retrieval speed is increased, a large amount of patent data can be effectively classified, the data utilization is more accurate, and the system security guarantee is effectively provided while the access, retrieval and query are facilitated.

The invention discloses a method and a device for extracting a gene fusion immune treatment neoantigen by integrating deep sequencing data of DNA and RNA. The method comprises the following steps: S10, acquiring a genome gene fusion sequence of a sample; S20, acquiring a transcriptome gene fusion sequence of the sample; S30, constructing a gene fusion proteome; and S40, obtaining a sample neoantigen. The tumor specific neoantigens discovered by the scheme of the invention are all from gene fusion, so that the screening range of the neoantigens is expanded, and an ammunition library of an immune treatment method based on the neoantigens is enriched. Through analysis and integration of tumor sample whole exome sequencing data and transcriptome sequencing data, gene fusion events in tumor tissues are comprehensively detected, the false positive rate of neoantigens generated by fusion is reduced, the effectiveness of neoantigen vaccine is improved, and the method is of great significance to improvement of the clinical immune treatment effect.

The invention discloses a terbinafine hydrochloride vaginal effervescent tablet and a preparation method thereof. The terbinafine hydrochloride vaginal effervescent tablet mainly comprises a basic remedy of terbinafine hydrochloride, an effervescent auxiliary material and other auxiliary materials, wherein the effervescent auxiliary material consists of organic acid and carbonate; and other auxiliary materials consist of one or more of a disintegrant, a binding agent, a diluting agent and a lubricating agent and a surfactant. The invention is suitable for women to administrate in vagina; and the medicament can be disintegrated in a shortest time, generates fine and even foam and is favorable for fully absorbing pharmacodynamical ingredients, so patients are easy to accept the medicament, and the medicament is suitable to be clinically popularized and applied. The invention also discloses the preparation method which ensures that the quality of products is stable, the preparation process is simple, the quality is controllable and the cost is lower, so the preparation method is suitable for industrial large-scale production. The terbinafine hydrochloride vaginal effervescent tablet prepared by the invention is mainly applied to monilial vaginitis and has unique effect.

The invention discloses a configuration method for undercurrent type artificial wetland woody plants in subtropical areas. The method includes: utilizing the characteristics that water of undercurrent type artificial wetland flows below 10-20cm of surface matrix to select from woody plants which can live through the winter safely in the subtropical areas according to staining resistance and decontamination capability, adaptability and physiological property indexes to obtain woody plants which can be applied to the undercurrent type artificial wetland, and configuring according to purification capability of the woody plants. The plants after configured can be engaged with various base materials, plants and microorganisms originally existing in the undercurrent type artificial wetland, andthe plants are configured to be oleander, hibiscus and gardenia. By the configuration method for the undercurrent type artificial wetland woody plants in the subtropical areas, the problem of withering of most herbaceous plants in artificial wetland in winter can be solved, selection range of artificial wetland plants is widened, and diversity of species is increased. The configuration method forthe undercurrent type artificial wetland woody plants in the subtropical areas is good in wastewater purification effect in the subtropical areas, can safely and effectively operate in the subtropical areas, and has good application prospects.

The invention relates to a method for generating a recombinant antibody combining at least one target antigen and the recombinant antibody generated thereby. The antibody generation method provided by the invention combines an optimized high-throughput antibody sequencing method with a hybridoma antibody screening technology, and compared with the traditional hybridoma monoclonal antibody technology, enlarges the selection range of candidate antibody genes, improves the antibody structure optimization efficiency, and increases the possibility of acquiring functional antibodies.

The invention relates to an educational institution recommendation method based on big data and a system thereof, the method comprising the following steps: an authentication module of a server verifies the identity of a user; the information obtaining module of the server obtains the education information of the user, the education information comprises: a school, an educational institution and aprofession; the verification module verifies the authentication of the educational institution; the information obtaining module obtains the identity and educational information of the user participating in the educational institution, and the curriculum of the user in the educational institution; the screening and sorting module divides the educational institution into a first sequence and a second sequence according to the user identity and educational information; the pushing module pushes an educational institution of a user whose educational information similarity degree exceeds a presetthreshold value to the user in a manner of a first sequence and a second sequence. The method further comprises the following steps: the information obtaining module obtains the number of courses ofa user in an educational institution; the information obtaining module obtains the attendance rate of the user to the course; the information obtaining module server obtains the satisfaction degree ofthe user to the educational institution.

The invention provides a method and system for calculating tumor neoantigen load. The method comprises the steps: S1, integrating normal and tumor data of a sample, sequencing a specific interval, anddetecting somatic mutation of the sample; S2, annotating and filtering the somatic mutation, and performing translating to obtain a mutant polypeptide sequence of the patient; S3, according to the normal proteome of the sample, filtering the mutant polypeptide sequence to obtain a new polypeptide sequence; and S4, performing human leukocyte antigen typing analysis according to the normal comparison data of the samples to obtain HLA genotypes of the samples; According to the method for calculating the tumor neoantigen load, weight scoring is performed on each predicted neoantigen, and high-quality and low-quality neoantigens can be distinguished by considering different dimension information such as mutation quality, mutation frequency, affinity and the like, so that a numerical value capable of better reflecting the real tumor neoantigen load of the sample is obtained.

An intelligent recommendation method and system for local educational institutions based on user similarity are provided. The method comprises the following steps: an authentication module of the server verifies the user identity; the information obtaining module of the server obtains the education information of the user, the education information comprises: a school, an educational institution and a profession; the verification module verifies the authentication of the educational institution; the information obtaining module obtains the identity and educational information of the user participating in the educational institution, and the curriculum of the user in the educational institution; the screening and sorting module divides the educational institution into a first sequence and asecond sequence according to the user identity and educational information; the pushing module pushes an educational institution of a user whose educational information similarity degree exceeds a preset threshold value to the user in a manner of a first sequence and a second sequence. The method further comprises the following steps: the information obtaining module obtains the number of coursesof a user in an educational institution; the information obtaining module obtains the attendance rate of the user to the course; the information obtaining module server obtains the satisfaction degree of the user to the educational institution.

The invention discloses a method for efficiently screening high linolenic acid germplasm of brassica napus. The method comprises the following steps of (1) expanding phenotypic variation of linolenicacid by utilizing a plurality of different ecological environments in winter and spring; (2) establishing different screening indexes according to different screening environments and generations; (3)performing comprehensive screening on the linolenic acid yield and the fatty acid composition to quickly obtain the high linolenic acid germplasm; (4) screening agronomic and quality characters whilescreening linolenic acid by utilizing a method of combining selfing and plant selection, so as to improve the comprehensive characters of the high linolenic acid germplasm; and (5) performing geneticstability analysis and quantitative evaluation on the many-year and multi-point results, so as to obtain the genetically stable high linolenic acid germplasm of brassica napus. The method disclosed by the invention has the characteristics of quantifiability and mechanical repeatability, can meet the screening requirements of the high linolenic acid germplasm of brassica napus, and can also enrichand complete the brassica napus breeding method; and meanwhile, beneficial reference can be provided for the development of fatty acid improvement work of other crops.

A vibrating bead screening machine is provided, which includes a base, a feed box and a discharge cabinet. The top of the base is symmetrically welded with vertical plates on the left and right sides, and a top plate is fixedly connected between the tops of the two vertical plates. Install the drive deceleration motor, install the flywheel on the output shaft of the drive deceleration motor, the edge of the flywheel is provided with a swing arm, the outer end of the swing arm is hinged with one end of the connecting rod, the other end of the connecting rod is hinged with the feed box, and the feed box The two sides are installed on the lifting slide rails inside the vertical plate through sliders; the top of the rear end of the feed box is provided with a feed inlet, and a feed hopper is welded at the feed inlet, and the bottom of the front end of the feed box is opened in turn. There are a first discharge port, a second discharge port and a third discharge port; the present invention drives the feeding box to vibrate up and down by driving the reduction motor and uses the screen inside the feeding box to automatically screen the beads, which has high efficiency.

The invention provides a method for quickly and efficiently screening a nisin high-yield strain. The method is characterized in that induced mutant lactococcus lactis servers as a starting strain, and low pH-Nisin resistance plate serves as a strain culture carrier for preliminary screening. The method is simple, and required agents are common; by adoption of the low pH resistance plate, screening efficiency is improved greatly through double indexes, a screening range is widened, possibility of screening out of the high-yield mutant strain is increased, and the grown-out strain is more representative; cell growth metabolite is treated to directly prepare a screening culture medium to screen a substrate tolerant strain, a substrate retroinhibition effect of cells is improved, cell growth and metabolism performances are improved, and the obtained strain is superior. By a re-screening step, all strains growing on the preliminary screening plate can be rescreened by one step, screening omission is avoided, and screening workload can be relieved effectively.

The invention provides a matching method of feature points in a robot vision navigation positioning image. The method comprises steps of extracting feature points from two frame images and performing rough matching of the feature points of the two frame images; and filtering the matching feature points using affine transformation invariance of spatial geometry. By using the feature that the captured ceiling image feature points have the affine transformation invariance between different frames, the mismatched feature points can be filtered according to whether the connection length of the feature pair in the two frame images and whether the corresponding angles are equal. The feature points on the ceiling can be screened, the accurate matching is realized, and the mismatched interference points can be removed. In addition, when the matching of the feature points of different frame images is made, that whether the length and the angle are equal are judged by using the threshold determination method, and the screening range of the effective feature points is enlarged, which is not limited to the ceiling feature points. The matching method is also applicable to the positioning in fuzzy, pixel discretization and other conditions of shooting.

The invention belongs to the technical field of prevention and treatment of melon fusarium wilt and discloses a method for screening and identifying a melon fusarium wilt resisting activity product in a Fosmid library. The method comprises steps as follows: soil microorganism genome DNA is extracted with an ectopic pyrolysis method; metagenome Fosmid library construction and quality detection are performed; a metagenome Fosmid expression library is screened, and Fosmid activity clone is obtained; a fermentation broth is extracted, and a relatively pure activity layer is obtained; purified melon fusarium wilt resisting activity substances are obtained; an atlas is analyzed, an activity substance structure is determined, and the melon fusarium wilt resisting activity of a compound is verified; endogenous strains of plants are screened and modified, genes are transferred to the modified endogenous strains, and an efficient expression system of melon fusarium wilt resisting drug genes is established by selecting different promoters, different media and fermentation conditions. The bottleneck that conventional biocontrol fungus cannot be subjected to field planting is broken through, and the research, development and commercialization process of new drugs for resisting melon fusarium wilt is greatly accelerated.

The invention relates to a cell screening method and device based on an expression quantity prediction model, computer equipment and a storage medium. The method comprises the following steps: acquiring to-be-detected cell grey-scale maps respectively corresponding to a plurality of to-be-detected cells in a cell culture pool, and acquiring target cell texture features respectively corresponding to the plurality of to-be-detected cell grey-scale maps, wherein the target cell texture feature is an optimal cell texture feature determined from a plurality of cell texture features in advance; inputting the target cell texture features of the plurality of cells to be detected into a pre-trained expression quantity prediction model, and obtaining predicted protein expression quantities respectively corresponding to the plurality of cells to be detected according to the output of the expression quantity prediction model; according to the predicted protein expression quantity, determining thetarget cells with the predicted protein expression quantity meeting the set conditions from the multiple cells to be detected. Cells with the high protein expression quantity are rapidly determined, it is avoided that cell screening can be conducted only after repeated culture and screening, and the screening period is greatly shortened.

The present application relates to a cell screening method, device, computer equipment and storage medium based on an expression level prediction model, including: acquiring grayscale images of cells to be tested corresponding to a plurality of cells to be tested in a cell culture tank, and acquiring a plurality of cells to be tested The target cell texture features corresponding to the grayscale images of the measured cells; the target cell texture features are the optimal cell texture features determined in advance from various cell texture features; the target cell texture features of multiple cells to be tested are input into the pre-trained cell texture features. Expression prediction model, and according to the output of the expression prediction model, the predicted protein expression corresponding to a plurality of cells to be tested is obtained; Conditional target cells can quickly determine cells with high protein expression, avoid the need to undergo repeated culture and screening before cell screening, and greatly shorten the screening cycle.

The invention relates to the technical field of screening, in particular to an article screening device based on industrial Internet of Things. The device comprises a middle plate, supporting legs are fixedly connected to two ends of one side of the upper surface of the middle plate, a second supporting plate is fixedly connected to the supporting legs, a first supporting plate is obliquely and fixedly connected to the connecting legs, and the first supporting plate and the second supporting plate form a screening channel. A fixing plate is fixedly connected to the first supporting plate, a limiting groove is formed in the fixing plate, the two vertical plates are arranged in a spaced mode, a worm is rotationally connected to the two vertical plates through bearings, a through hole is formed in the edge of a worm gear, a connecting rod can be inserted into the through hole, a hinge seat is fixedly connected to the connecting rod, and a supporting mechanism is arranged at the end, close to the hinge seat, of a moving edge. A plurality of supporting rods are fixedly connected to the position under the screening channel at equal intervals, and a material guiding sliding way is fixedly connected to the multiple supporting rods. The device has the beneficial effects that the screening range can be conveniently adjusted, and therefore articles of different sizes can be screened.

The invention discloses a system and method for pushing global information in real time based on a micro platform, and relates to the technical field of information analysis, processing and pushing.The system comprises a micro platform for whole-region information annalists and pushing; an enterprise terminal used for receiving the information pushed by the micro platform; wherein the enterprise terminal is also used for inputting keyword information of the wanted global information to the micro platform; and internet used for providing network files for the micro platform. The invention is scientific, reasonable and safe and convenient to use, and by comparing and analyzing the internet public files and the enterprise keywords, on one hand, the enterprise is assisted to classify and screen network information pools, and accurate positioning is conducted on acquisition of external information of the enterprise; on the other hand, beneficial benchmarking information can be obtained for an enterprise in real time, repeated implantation of a certain piece of information by the enterprise is avoided, and benchmarking and difference finding effects are indirectly achieved; finally, information source support can be provided for the enterprise from other aspects, and a way is laid for subsequent development of the enterprise.

The invention relates to an evaluation method for performance of hydrate accelerant, in particular to a method for theoretically evaluating the hydrate forming performance of the hydrate accelerant and predicting the crystal structure of hydrate, wherein starting from the bond forming nature in the forming process of the hydrate, energy of the highest occupied orbital of accelerant molecules and the lowest unoccupied orbital of water molecules, the ratio of diameter of the accelerant molecules to diameter of the hydrate molecules and whether hydrogen bond or halogen bond influence is formed between accelerant molecules and water molecules or not are analyzed to theoretically evaluate the hydrate forming performance of the hydrate accelerant and predict the crystal structure of the hydrate.

The present application relates to a cell screening method and device based on a convolutional neural network, the method comprising: acquiring grayscale images of cells to be tested corresponding to a plurality of cells to be tested in a cell culture pool; Multiple grayscale images of the cells to be tested are input to the target convolutional neural network model; according to the output of the target convolutional neural network model, the protein expression levels corresponding to the multiple cells to be tested are obtained; according to the corresponding The protein expression level is high, and the target cells whose protein expression level meets the set conditions are determined from the multiple cells to be tested, which realizes the rapid determination of cells with high protein expression level, and avoids the need for repeated culture and screening before the cell Screening, greatly shortened screening cycle.