Biological culture device

A biological culture and culture room technology, applied in the field of screening devices for antagonistic organisms and biological metabolites, and biological culture devices, can solve the problems of inability to observe antagonistic phenomena, inability to isolate and limit the water phase, and achieve convenient confrontation culture, Increase the screening range and design a reasonable effect

Inactive Publication Date: 2015-06-10
CHINA NAT RICE RES INST
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AI Technical Summary

Problems solved by technology

The reality is that different organisms often require different culture substrates, so the common culture substrate must be screened in advance, but the target organism and the organism to be screened may not grow well on the same medium, which brings difficulties to the screening test work. hassles and limitations
Furthermore, ordinary confrontation culture can only use solid medium because there is only one culture chassis. If liquid medium is added, since there is no solid support, the target organism and the organism to be screened may be mixed as soon as they are put in, making it impossible to observe. Antagonism phenomenon, and because the water phase cannot be isolated, it cannot be ruled out whether the active metabolite comes from the water phase, and it cannot be used for the screening of organisms that produce volatile metabolites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening and identification of bacteria producing active volatile substances resistant to rice blast and evaluation of their inhibitory ability

[0026] 1 strain

[0027] Both strain A832 and strain A841 were isolated from paddy field soil; rice blast strain GXA-31 was isolated from rice blast specimens in Guangxi; the above strains were all preserved by the pathology laboratory of China Rice Research Institute.

[0028] 2 Test method

[0029] Pour the PDA medium into the inner culture chamber and the outer culture chamber respectively, and form a flat plate after cooling.

[0030] Inoculate the 0.5cm rice blast fungus cake on the edge of the inner culture room, use sterile water to prepare the bacterial suspensions of strain A832 and strain A841, and spread them in the outer culture room respectively. For the control CK, only spread the same amount of aseptic water. 28 o M. oryzae cultured from C to CK grew close to the edge of the petri dish. Investiga...

Embodiment 2

[0033] Example 2 Extraction and property research of active volatile substances

[0034]The strain A841 was coated in the outer culture chamber, and sterile water and acetone were injected into the inner culture chamber respectively, and cultured at 30oC for 10 days. The mycelium inhibition test shows that there is no active substance inhibiting the rice blast bacterium in sterile water, but the acetone contains such substances, indicating that the acetone crude extract of the volatile active substance of the bacterial strain A841 has been obtained, and it can be explained that the active metabolite is lipid Soluble, the content of such substances can also be further determined by gas chromatography, and the substance can be purified by preparative liquid chromatography.

Embodiment 3

[0035] Example 3 Determination of potency of volatile pesticides.

[0036] Inject volatile pesticides into the inner or outer culture room, and cultivate the mycelia or spores of pathogenic fungi and lower animals such as nematodes in another culture room. The mortality rate can be measured to evaluate the efficacy of the pesticide.

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Abstract

The invention provides a biological culture device and belongs to the technical field of experiment devices. The biological culture device comprises a bottom disc and a top cover, wherein the bottom disc comprises an outer disc and more than one inner disc arranged in the outer disc; and the outer disc and the inner disc are used for dividing the bottom disc into a plurality of outer culture rooms and inner culture rooms. By virtue of the device, an operator can conveniently carry out confront culture in screening work of volatile active substances and the confront culture effect is good; and the biological culture device is particularly suitable for identification and detection of the produced volatile active substances, screening and identification of organisms capable of producing volatile active metabolites and the like.

Description

technical field [0001] The invention belongs to the technical field of experimental equipment, specifically relates to a biological cultivation device, belongs to the field of biological source pesticide development, and specifically relates to a screening device for antagonistic organisms and biological source metabolites, and is mainly used in the screening and production of volatile active metabolites. detection work. Background technique [0002] Culture vessels are indispensable devices in biological related laboratories. Petri dish, also known as "Petri dish" or "Petri dish", was originally developed by bacteriologist Julius Richard Petri, who worked under German biologist Robert Koch Designed by Julius Richard Petri (1852-1921) in 1887, the device consists of a flat disk-shaped bottom and a cover, generally made of glass or plastic, and is the most commonly used and simplest culture device. Widely used in the culture of microorganisms, protozoa and cells. [0003] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M1/22
Inventor 刘连盟黄世文王玲
Owner CHINA NAT RICE RES INST
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