Cell screening method and device based on convolutional neural network

Abstract

The present application relates to a cell screening method and device based on a convolutional neural network, the method comprising: acquiring grayscale images of cells to be tested corresponding to a plurality of cells to be tested in a cell culture pool; Multiple grayscale images of the cells to be tested are input to the target convolutional neural network model; according to the output of the target convolutional neural network model, the protein expression levels corresponding to the multiple cells to be tested are obtained; according to the corresponding The protein expression level is high, and the target cells whose protein expression level meets the set conditions are determined from the multiple cells to be tested, which realizes the rapid determination of cells with high protein expression level, and avoids the need for repeated culture and screening before the cell Screening, greatly shortened screening cycle.

Description

technical field [0001] The present application relates to the field of biotechnology, in particular to a convolutional neural network-based cell screening method, device, computer equipment and storage medium. Background technique [0002] With the continuous development of genetic engineering technology, it has become a common requirement in the biological field to isolate monoclonal cell lines capable of expressing specific products from cell pools. [0003] In the prior art, when obtaining cells for cultivating monoclonal cell lines, the cells in the cell pool can be transfected first, and the cell pool can be treated with the limiting dilution method to obtain a single cell, and then a single cell can be used. Cell culture has a homogeneous cell population, that is, cell lines, and screens the cell lines with high expression of the target protein. [0004] However, the process of obtaining single cells using the limiting dilution method is cumbersome and requires repeat...

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Problems solved by technology

[0004] However, the process of obtaining single cells using the limiting dilution method is cumbersome and requires repeated culture and screening. At the same time, due to the problem of cell transfection efficiency, the proportion of cells with high expression levels of the target protein is low, resulting in low efficiency of screening cells. The screening cycle is long, and it is difficult to quickly and accurately obtain cells with high expression of the target protein

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