Cell screening method and device based on expression level prediction model

Abstract

The present application relates to a cell screening method, device, computer equipment and storage medium based on an expression level prediction model, including: acquiring grayscale images of cells to be tested corresponding to a plurality of cells to be tested in a cell culture tank, and acquiring a plurality of cells to be tested The target cell texture features corresponding to the grayscale images of the measured cells; the target cell texture features are the optimal cell texture features determined in advance from various cell texture features; the target cell texture features of multiple cells to be tested are input into the pre-trained cell texture features. Expression prediction model, and according to the output of the expression prediction model, the predicted protein expression corresponding to a plurality of cells to be tested is obtained; Conditional target cells can quickly determine cells with high protein expression, avoid the need to undergo repeated culture and screening before cell screening, and greatly shorten the screening cycle.

Description

technical field [0001] The present application relates to the field of biotechnology, in particular to a cell screening method, device, computer equipment and storage medium based on an expression level prediction model. Background technique [0002] With the continuous development of genetic engineering technology, the isolation of monoclonal cell lines capable of expressing specific products from cell pools has become a common requirement in the biological field. [0003] In the prior art, when obtaining cells for culturing a monoclonal cell line, the cells in the cell pool can be transfected first, and the cell pool can be processed by a limiting dilution method to obtain a single cell, and then a single cell can be obtained. Cells are cultured with a homogenous cell population, that is, cell lines, and the cell lines with high target protein expression are screened. [0004] However, the process of obtaining single cells by limiting dilution method is cumbersome and req...

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Problems solved by technology

[0004] However, the process of obtaining single cells using the limiting dilution method is cumbersome and requires repeated culture and screening. At the same time, due to the problem of cell transfection efficiency, the proportion of cells with high expression levels of the target protein is low, resulting in low efficiency of screening cells. The screening cycle is long, and it is difficult to quickly and accurately obtain cells with high expression of the target protein

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