Cell screening method and device based on expression quantity prediction model, computer equipment and storage medium

Abstract

The invention relates to a cell screening method and device based on an expression quantity prediction model, computer equipment and a storage medium. The method comprises the following steps: acquiring to-be-detected cell grey-scale maps respectively corresponding to a plurality of to-be-detected cells in a cell culture pool, and acquiring target cell texture features respectively corresponding to the plurality of to-be-detected cell grey-scale maps, wherein the target cell texture feature is an optimal cell texture feature determined from a plurality of cell texture features in advance; inputting the target cell texture features of the plurality of cells to be detected into a pre-trained expression quantity prediction model, and obtaining predicted protein expression quantities respectively corresponding to the plurality of cells to be detected according to the output of the expression quantity prediction model; according to the predicted protein expression quantity, determining thetarget cells with the predicted protein expression quantity meeting the set conditions from the multiple cells to be detected. Cells with the high protein expression quantity are rapidly determined, it is avoided that cell screening can be conducted only after repeated culture and screening, and the screening period is greatly shortened.

Description

technical field [0001] The present application relates to the field of biotechnology, in particular to a cell screening method, device, computer equipment and storage medium based on an expression level prediction model. Background technique [0002] With the continuous development of genetic engineering technology, the isolation of monoclonal cell lines capable of expressing specific products from cell pools has become a common requirement in the biological field. [0003] In the prior art, when obtaining cells for culturing a monoclonal cell line, the cells in the cell pool can be transfected first, and the cell pool can be processed by a limiting dilution method to obtain a single cell, and then a single cell can be obtained. Cells are cultured with homogeneous cell populations, namely cell lines, and the cell lines with high target protein expression are screened. [0004] However, the process of obtaining single cells by the limiting dilution method is cumbersome and r...

AI Technical Summary

Problems solved by technology

[0004] However, the process of obtaining single cells using the limiting dilution method is cumbersome and requires repeated culture and screening. At the same time, due to the problem of cell transfection efficiency, the proportion of cells with high expression levels of the target protein is low, resulting in low efficiency of screening cells. The screening cycle is long, and it is difficult to quickly and accurately obtain cells with high expression of the target protein

Images