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Method for separating electrochemical activity bacterial strain and detecting electrochemical activity

A separation method and electrochemical technology, applied in the field of microbial separation and inspection, can solve the problems of cumbersome inspection operation of electrochemically active strains, unfavorable growth of electrochemically active strains, difficulty in obtaining high-efficiency electricity-producing bacteria, etc., so as to shorten the screening period. , The effect of rich composition, saving time and effort

Inactive Publication Date: 2009-04-15
HARBIN INST OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

[0007] The invention discloses an isolation and inspection method for electrochemically active bacterial strains, which solves the problem that the single composition of the culture medium in the isolation of electrochemically active bacterial strains is not conducive to the growth of electrochemically active bacterial strains, the success rate of separation and purification is low, and it is difficult to obtain high-efficiency production. Electrobacteria, electrochemically active strains inspection operation is cumbersome and the screening period is long

Method used

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  • Method for separating electrochemical activity bacterial strain and detecting electrochemical activity
  • Method for separating electrochemical activity bacterial strain and detecting electrochemical activity
  • Method for separating electrochemical activity bacterial strain and detecting electrochemical activity

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specific Embodiment approach 1

[0013] Specific embodiment one: the separation of the electrochemically active bacterial strain of this embodiment is carried out according to the following steps: one, under aseptic condition, cut off the anode of the microbial fuel cell, then drop into the bottle containing the liquid culture medium of sterilization, seal and place Cultivate for 2 to 4 days at 25-32°C; 2. Take 1 mL of the bacterial solution obtained in Step 1 for doubling dilution; The bacterial liquid is evenly distributed on the entire solid medium, and then cultivated at 28-31°C for 5-10 days; 4. Pick a single colony in the Henggate rolling tube in step 3 and inoculate it in the liquid medium, and inoculate it at 25-31°C. Cultivate at 32°C for 2 to 4 days, and the obtained bacterial solution is an electrochemically active strain; wherein every 1000 mL of liquid medium in step 1 contains 10 to 20 g of glucose, 2 to 4 g of tryptone, and 2 to 4 g of Beef extract, 1-1.5g of yeast, 3-6g of NaCl, 1-2g of K 2 H...

specific Embodiment approach 2

[0015] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, under aseptic conditions, cut off 2-5mm 2 Anode of the microbial fuel cell. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0016] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the multiple dilution ratio in step two is 10 3 ~10 7 times. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses an electrochemically active strain separating method and an electrochemical activity detecting method, relates to microorganism separating and detecting methods, and solves the problems that separating culture mediums of electrochemically active strains have simple nutrition, are unfavorable for the growth of the electrochemically active strains, and has low electrochemical activity and success ratio of the separated strains, complex detecting operations of electrochemical strain activity and long filtering period. The separating method comprises the steps of: (1) accumulation cultivation, (2) doubling dilution, (3) Hungate rolled tube separation and (4) electrochemically active strain purification. A circular voltammetry method is adopted for detecting electrochemical strain activity. A liquid culture medium of the electrochemically active strain separating method has abundant nutrition, favorable growth of the electrochemically active strains, very intuitive strain size and morpha observation and high separating success ratio; and the circular voltammetry method adopted for detecting the electrochemically active strains has simple operations and shortened filtering period.

Description

technical field [0001] The invention relates to a method for separating and testing microorganisms. Background technique [0002] Microbial fuel cell is a new type of device that uses biomass and microorganisms to produce electrical energy. Through the catalytic oxidation of microorganisms, the direct conversion of chemical energy and electrical energy is realized. During the entire energy conversion process, the anode biofilm formed by microorganisms is The key part of power generation is also the speed limiting link. The electrochemically active strain can quickly adsorb on the surface of the anode, grow and reproduce, and greatly shorten the time for the formation of biofilm on the anode. More importantly, its activity will directly affect the output voltage and energy conversion efficiency of the battery. Under the same external conditions, the strain with high activity can make the substrate fully utilized in a short time, the energy conversion rate is high, and the ou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12Q1/04
Inventor 冯玉杰李贺于艳玲刘尧兰何伟华
Owner HARBIN INST OF TECH
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