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126results about How to "The inspection method is simple" patented technology

Method for detecting sulfanilamide medicine and special enzyme-linked immunoassay reagent kit thereof

The invention discloses a method for detecting a sulfanilamide medicine and a special enzyme-linked immunoassay reagent kit thereof. The enzyme-linked immunoassay reagent kit comprises a sulfanilamide medicine monoclonal antibody and the sulfanilamide medicine. The sulfanilamide medicine monoclonal antibody is secreted by the hybridoma cell line SAs of a sulfanilamide monoclonal antibody, whose preservation number is CGMCC No. 3393. In the enzyme-linked immunoassay reagent kit of the invention, an indirect competition ELISA (Enzyme-Linked Immuno Sorbent Assembly) method is mainly used for qualitatively or quantitatively detecting the content of the sulfanilamide medicine in products (especially milk, pork, chicken, eggs, honey, fish, shrimp and the like) eaten by animals or people. The reagent kit and the detection method of the invention have low requirements on the pretreatment of samples and simple pretreatment process of the samples and can detect mass samples quickly at the same time. By using the sulfanilamide medicine monoclonal antibody with high specificity, the detection method is convenient and simple, and the invention has the characteristics of high specificity, high sensibility, high precision, high accuracy and the like.
Owner:北京维德维康生物技术有限公司

Method and system for achieving steering-wheel turning angle verification based on EPS

The invention relates to the technical field of automobile control, in particular to a method and system for achieving steering-wheel turning angle verification based on EPS. A target turning angle command is received, and according to the target turning angle command, a motor of a steering wheel is driven so as to make the steering wheel rotate to a target angle; the actual turning angle deltaalpha of the steering wheel in a sampling time interval deltat is calculated; the turning angle deltadelta of a motor in the sampling time interval deltat is calculated; the turning angle deltadelta of amotor in the sampling time interval deltat is converted into the relative turning angle deltabeta of the steering wheel; the difference between the relative turning angle deltabeta of the steering wheel and the actual turning angle deltaalpha of the steering wheel is calculated, when the absolute value of the difference is not greater than that of phi, it is judged that a turning angle signal ofthe steering wheel is normal, and when the absolute value of the difference is greater than phi, it is judged that the turning angle signal of the steering wheel is abnormal. According to the rotary position sensor signal of a motor of the steering wheel in an electric power steering system, the relative turning angle of the steering wheel is calculated out, and the redundancy check of a steeringwheel angle signal sent by a vehicle angle sensor is achieved.
Owner:DONGFENG MOTOR CORP HUBEI

Enzyme-linked immunoassay kit for chloramphenicol detection, and applications thereof

The present invention provides an enzyme-linked immunoassay kit for chloramphenicol detection. The enzyme-linked immunoassay kit comprises an enzyme label plate coated with coating antigen, an enzyme marker, a specific chloramphenicol antibody working solution (when the enzyme label plate is coated with antigen and the enzyme marker is enzyme-labeled anti-antibody or the enzyme label plate is coated with anti-antibody and the enzyme marker is enzyme-labeled antigen, the kit contains the specific chloramphenicol antibody working solution), chloramphenicol standard solutions, a substrate coloration solution, a termination solution, a concentration washing solution and a concentration reconstituted solution. The present invention further discloses a method for detecting chloramphenicol by using the enzyme-linked immunoassay kit. The method comprises: carrying out a sample pretreatment, adopting the kit to detect, and finally analyzing a detection result. With the enzyme-linked immunoassay kit, chloramphenicol residue in animal tissues (muscles, livers and the like), delicatessen, aquatic products, royal jelly, urine, feeds, milk and other samples can be detected, characteristics of simple operation, low cost and high sensitivity are provided, and the kit and the method can be applicable for screening and on-site monitoring of a large number of samples.
Owner:BEIJING KWINBON BIOTECH

Swine fever and porcine pseudorabies bivalent vaccine as well as preparation method and application thereof

InactiveCN103505724ASolve the problem of low early potencyImprove securityAntiviralsAntibody medical ingredientsDiseaseRabies
The invention provides a swine fever and porcine pseudorabies bivalent vaccine. The swine fever and porcine pseudorabies bivalent vaccine contains at least one swine fever virus antigen and at least one porcine pseudorabies virus antigen, wherein the two antigens coordinate well, are excellent in immune effect and can promote each other. The swine fever and porcine pseudorabies bivalent vaccine is simple in preparation method, is convenient and efficient in immunization and has the advantages that immunization cost is reduced, an immunization procedure is simplified and economy and reliability are realized compared with a vaccine which can be used for preventing and treating more than two diseases only when immunization is carried out in steps and at least two injections are taken and an immune method of the vaccine in the prior art. The immune effect of the swine fever and porcine pseudorabies bivalent vaccine is better than that of a single vaccine and better in safety and avoids adverse effects caused by multiple immunizations. Besides, the invention also provides a simple testing method for determining swine fever effect in the bivalent vaccine by adopting an indirect immunofluorescence method, so that quality of bivalent live vaccines in each batch is guaranteed, and economic benefit is obviously increased.
Owner:PU LIKE BIO ENG
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