A kit for early prediction of osteoporosis

A technology for osteoporosis and a kit, which is applied in the field of biotechnology applications, can solve the problems of lack of specific detection indicators for osteoporosis, and achieve high accuracy, simple operation, and good reliability

Active Publication Date: 2018-06-29
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a lack of ideal specific detection indicators for early clinical prediction of osteoporosis.

Method used

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  • A kit for early prediction of osteoporosis
  • A kit for early prediction of osteoporosis
  • A kit for early prediction of osteoporosis

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The present invention will be further described below in conjunction with drawings and embodiments.

[0025] (1) Discovery of the early diagnostic marker IFNg (interferon-γ) for osteoporosis

[0026] The present invention adopts the proteomics technology to detect the protein markers whose serum content changes significantly in the early stage of postmenopausal osteoporosis, and provides the possibility for convenient and economical early prediction of osteoporosis.

[0027] 1. Grouping of experimental animals

[0028] Twenty SD rats were randomly divided into OVX group and Sham group according to the random table method, with 10 rats in each group. The OVX group is an ovariectomy (castration) rat model as an animal model, which is a commonly used model for simulating postmenopausal osteoporosis in women; the Sham group is a sham operation control group, in which an equal amount of fat is removed around the ovaries of rats as a sham operation control. All rats were a...

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Abstract

The invention provides a kit for early prediction of osteoporosis. The kit comprises a detection plate, a standard substance, interferon-gamma antibodies I and interferon-gamma antibodies II capable of emitting fluorescent light, wherein the detection plate comprises a plate body and at least two sample adding holes in the plate body; the bottoms of the sample adding holes are coated with the interferon-gamma antibodies II; the standard substance is an interferon-gamma water solution, and the concentration of the water solution is higher than or equal to 1 ng / mL. A serum sample of a to-be-tested person and the standard substance are added to the sample adding holes in the detection plate respectively, and whether the to-be-tested person has the risk of osteoporosis is judged according to the difference of fluorescence intensity. The kit is low in cost, high in accuracy and good in reliability, has a risk prediction function by comparison with traditional bone density examination and can provide a diagnosis basis for prediction of osteoporosis in clinic.

Description

technical field [0001] The invention belongs to the application field of biotechnology, and in particular relates to early prediction of osteoporosis. Background technique [0002] Osteoporosis (OP) is a systemic bone metabolic disease characterized by low bone mass and microarchitectural destruction of bone tissue, and clinically manifested by increased bone fragility. [0003] Osteoporosis can be divided into primary and secondary types: primary osteoporosis refers to osteoporosis that occurs without other diseases that cause the disease, postmenopausal osteoporosis and senile osteoporosis It is the main type of primary osteoporosis; secondary osteoporosis refers to osteoporosis caused by any disease, drug or organ transplantation that affects bone metabolism. In addition, according to the site of occurrence, it can also be divided into localized or generalized osteoporosis. The incidence of osteoporosis ranks first among the common orthopedic diseases of middle-aged and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6866G01N2333/57G01N2800/108G01N2800/50
Inventor 杨柳胡雅茜罗卓荆赵卓杰
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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