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Nano-gold test paper for detecting major outer membrane protein antigen of chlamydia trachomatis as well as preparation method and application thereof

A technology for the main outer membrane protein and Chlamydia trachomatis, which is applied in the field of test strips for detecting Chlamydia trachomatis infection, can solve the problems of rapid detection test strips of gold nanoparticles that have not been seen, and achieves good application prospects, high specificity and good sensitivity.

Inactive Publication Date: 2012-04-11
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only ELISA kits for detecting Ct MOMP on the market, and there are no reports of nano-gold rapid detection test strips for detecting Ct MOMP.

Method used

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  • Nano-gold test paper for detecting major outer membrane protein antigen of chlamydia trachomatis as well as preparation method and application thereof
  • Nano-gold test paper for detecting major outer membrane protein antigen of chlamydia trachomatis as well as preparation method and application thereof
  • Nano-gold test paper for detecting major outer membrane protein antigen of chlamydia trachomatis as well as preparation method and application thereof

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Experimental program
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Embodiment Construction

[0026] 1 Preparation of test materials

[0027] Chlamydia trachomatis MOMP Antigen Mouse Anti-Human Monoclonal Antibody IgG (Antibody II), Cat. No. C55180M, produced by Biodesign, with a titer of 1:200,000; Goat Anti-Mouse Polyclonal Antibody (Goat Anti-Mouse Polyclonal Antibody), Cat. No. 101-0301 , produced by Tianjin Biotechnology Co., Ltd.; the Chlamydia trachomatis MOMP antigen mouse anti-human monoclonal antibody IgG (antibody I), product number C55135M, titer 1:200000, produced by Biodesign.

[0028] The preparation method of 2 nanometer gold test paper

[0029] (1) Pretreatment of nitrocellulose membrane and conjugation pad

[0030] Spray the mouse anti-human monoclonal antibody II of Chlamydia trachomatis MOMP antigen with a concentration greater than or equal to 1 mg / mL on the nitrocellulose membrane with an XYZ-3000 three-dimensional spraying device to form a detection line, and the concentration greater than or equal to 1.5 mg / mL Goat anti-mouse polyclonal...

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Abstract

The invention relates to test paper for detecting chlamydia trachomatis infection, and the test paper comprises a baseplate, a sample pad, a conjugate pad, a nitrocellulose membrane and an absorption pad, wherein a detection line and a quality control line are arranged on the nitrocellulose membrane, the detection line on the nitrocellulose membrane is coated by mouse anti-human monoclonal protein antibody IgG (antibody II) of major outer membrane protein antigen of chlamydia trachomatis in the concentration of greater than or equal to 1mg / mL, and the quality control line is coated by goat anti-mouse IgG polyclonal antibody in the concentration of greater than or equal to 1.5mg / mL; the conjugate pad is coated by mouse anti-human monoclonal protein antibody IgG (antibody I) of the MOMP (major outer membrane protein) antigen of the chlamydia trachomatis, which is labeled by nano-gold and is in the concentration of not less than 6 mu g / mL; the sensitivity of detecting the MOMP of the chlamydia trachomatis of the test paper is 0.1ng / ml, the detection value range is 0-200ng / ml and the specificity is 98.5%; and the detection can be completed within 5 minutes.

Description

technical field [0001] The invention relates to the field of disease detection devices, in particular to a test paper for detecting chlamydia trachomatis infection. Background technique [0002] Chlamydia trachomatis (Ct) has a unique developmental cycle, with two forms: Elementary Body and Reticulate Body. Its unique life cycle is that the protoplasma-infected cells develop into reticulum, and the reticulum divides into protoplasma to be released, and then re-infects cells. Ct is a pathogen that survives in the human body for a long time and spreads widely. It is one of the main pathogens of sexually transmitted diseases (STDs) in my country. Infections and other diseases. Studies have found that Ct infection is still related to the onset of many autoimmune diseases, such as chlamydia infection can cause reactive arthritis, Reiter syndrome and so on. [0003] A, B, C or Ba antigen type Ct infection can also lead to a blinding chronic infectious conjunctiva keratitis, name...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/531
Inventor 方立超郑峻松程平黄辉蒋丽莉李艳邓均贺娟
Owner ARMY MEDICAL UNIV
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