Nano-gold test paper for detecting major outer membrane protein antigen of chlamydia trachomatis as well as preparation method and application thereof

Abstract

The invention relates to test paper for detecting chlamydia trachomatis infection, and the test paper comprises a baseplate, a sample pad, a conjugate pad, a nitrocellulose membrane and an absorption pad, wherein a detection line and a quality control line are arranged on the nitrocellulose membrane, the detection line on the nitrocellulose membrane is coated by mouse anti-human monoclonal protein antibody IgG (antibody II) of major outer membrane protein antigen of chlamydia trachomatis in the concentration of greater than or equal to 1mg / mL, and the quality control line is coated by goat anti-mouse IgG polyclonal antibody in the concentration of greater than or equal to 1.5mg / mL; the conjugate pad is coated by mouse anti-human monoclonal protein antibody IgG (antibody I) of the MOMP (major outer membrane protein) antigen of the chlamydia trachomatis, which is labeled by nano-gold and is in the concentration of not less than 6 mu g / mL; the sensitivity of detecting the MOMP of the chlamydia trachomatis of the test paper is 0.1ng / ml, the detection value range is 0-200ng / ml and the specificity is 98.5%; and the detection can be completed within 5 minutes.

Description

technical field [0001] The invention relates to the field of disease detection devices, in particular to a test paper for detecting chlamydia trachomatis infection. Background technique [0002] Chlamydia trachomatis (Ct) has a unique developmental cycle, with two forms: Elementary Body and Reticulate Body. Its unique life cycle is that the protoplasma-infected cells develop into reticulum, and the reticulum divides into protoplasma to be released, and then re-infects cells. Ct is a pathogen that survives in the human body for a long time and spreads widely. It is one of the main pathogens of sexually transmitted diseases (STDs) in my country. Infections and other diseases. Studies have found that Ct infection is still related to the onset of many autoimmune diseases, such as chlamydia infection can cause reactive arthritis, Reiter syndrome and so on. [0003] A, B, C or Ba antigen type Ct infection can also lead to a blinding chronic infectious conjunctiva keratitis, name...

AI Technical Summary

Problems solved by technology

At present, there are only ELISA kits for detecting Ct MOMP on the market, and there are no reports of nano-gold rapid detection test strips for detecting Ct MOMP.

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