Application of selective cell-removed pork skin as skin substitute of human body and its preparation method
A technology for human skin and decellularization, applied in the field of pig skin, can solve the problems of high cost, easy infection, high price, etc., and achieve the effects of low price, easy preparation and wide source.
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[0044] The method for preparing the decellularized pigskin of the present invention as a human skin substitute takes about 50 kg of healthy white domestic pigs without skin diseases, kills them alive, shaves and washes the skin, removes a large full-thickness skin, and cuts it back to 0.3-0.4mm The skin slices were cross-linked with 0.5% glutaraldehyde for 10 minutes, washed 5 times with phosphate balanced saline, then soaked in IM physiological saline containing 0.25% trypsin at 37°C for 2 hours, and then placed in 0.5% Triton Shake in -100 distilled water at 37°C 100 times / min for 36 hours, wash with balanced phosphoric acid saline for 5 times, and store in a 4°C refrigerator for later use.
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