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Method for extracting pigskin collagen peptides

A technology of pigskin collagen and its extraction method is applied in the field of deep processing of pigskin, which can solve the problems of cumbersome operation and damage, and achieve the effects of high purity, short production cycle and easy absorption

Inactive Publication Date: 2011-08-17
湖北远成赛创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the method for extracting pigskin collagen peptides generally uses chemical means to degrease twice (such as Chinese patent application 200810061250.0), which is cumbersome and inevitably leads to the destruction of amino acid and other material structures.

Method used

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  • Method for extracting pigskin collagen peptides
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  • Method for extracting pigskin collagen peptides

Examples

Experimental program
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Embodiment 1

[0023] A method for extracting pigskin collagen peptides, the steps are as follows:

[0024] Weigh 1kg of fresh pigskin, wash it with 40°C water, add 7kg of pure water, then add 15g of anhydrous sodium sulfite, stir evenly, heat up to 100°C and reflux for 4 hours, then filter while it is hot; then cool the filtrate to 55°C, use 0.1M food-grade flake sodium hydroxide solution to adjust the pH to 7.5, add 30g lipase, keep the temperature at 55°C, stir for 8 hours, and use 0.1M food-grade flake sodium hydroxide solution to keep the pH value of the system stable during the reaction ; Then add 15g of alkaline protease, use 0.1M food-grade sodium hydroxide solution to keep the pH value of the reaction system at 7.5, keep the temperature at 55°C, and stir for 8 hours; Filtrate the solution to obtain a supernatant; add 20 g of powdered activated carbon to the supernatant, keep the temperature at 42°C for 1 hour, stir and absorb for decolorization, and filter out the activated carbon; ...

Embodiment 2

[0028] A method for extracting pigskin collagen peptides, the steps are as follows:

[0029] Weigh 2kg of fresh pigskin, wash it with water at 38°C, add 15kg of pure water, add 40g of anhydrous sodium sulfite, stir evenly, heat up to 100°C and reflux for 4 hours, then filter while it is hot; cool the filtrate to 60°C, and use Adjust the pH to 7 with 0.1M food-grade flake sodium hydroxide solution, add 50g of lipase, keep the temperature at 60°C, stir for 7 hours, and use 0.1M food-grade flake sodium hydroxide solution to keep the pH value of the system stable during the reaction ; Then add 40g of alkaline protease, keep the pH value of the reaction system at 7 with 0.1M food-grade flake sodium hydroxide solution, keep the temperature at 60°C, stir for 8 hours; then raise the temperature to 90°C, keep warm for 20min to kill the enzyme; Filtrate the solution to obtain a supernatant; add 40 g of powdered activated carbon to the supernatant, keep the temperature at 45°C for 1 hour...

Embodiment 3

[0033] A method for extracting pigskin collagen peptides, the steps are as follows:

[0034] Weigh 10kg of fresh pigskin, wash it with water at 45°C, add 90kg of pure water, add 160g of anhydrous sodium sulfite, stir evenly, heat up to 100°C and reflux for 5 hours, then filter while it is hot; cool the filtrate to 50°C, and use Adjust pH to 8 with 0.1M food-grade flake sodium hydroxide solution, add 280g lipase, keep the temperature at 50°C, stir for 9 hours, and use 0.1M food-grade flake sodium hydroxide solution to keep the pH value of the system stable during the reaction ; Then add 150g of alkaline protease, keep the pH value of the reaction system at 8 with 0.1M food-grade flake sodium hydroxide solution, keep the temperature at 50°C, and stir for 10 hours; Filtrate the solution to obtain a supernatant; add 220 g of powdered activated carbon to the supernatant, keep the temperature at 44°C, stir and adsorb for 1 hour to decolorize, and filter out the activated carbon; the...

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Abstract

The invention discloses a method for extracting pigskin collagen peptides. The method comprises the following steps that: fresh pig skin serving as a main raw material undergoes cleaning, water addition, addition of sodium sulfite and boiling, filtration, defatting of lipase, enzymolysis of protease, enzymic inactivation, decoloring and membrane separation, and the obtained filtrate is dried to form the pigskin collagen peptides. The method has the advantages of short production period, low cost, mild reaction conditions and environmental friendliness, and the products prepared by the method are high in safety and widely applied in the fields of healthcare products, cosmetics, medicines and the like.

Description

technical field [0001] The method of the invention relates to the technical field of deep processing of pigskin, in particular to a method for extracting pigskin collagen peptides, which is a method for obtaining pigskin collagen peptides by using fresh pigskin as a raw material by purely biological means. Background technique [0002] Pig skin, a by-product of pork production, is heat-treated during pork processing in meat factories and cannot be used for leather manufacturing. Wherein the protein content is as high as more than 33%, and the collagen protein content is about 88%, and its nutritional value is very considerable. Due to the special and complex spatial structure of collagen, its molecular weight is as high as 300KD. Compared with collagen peptides, it is difficult to be absorbed by the human body. Therefore, collagen is hydrolyzed into small molecular polypeptide substances by biological means to develop products that are beneficial to the human body. Has broa...

Claims

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Application Information

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IPC IPC(8): C12P21/06B01D61/00
Inventor 叶传发张小东胡承四叶思
Owner 湖北远成赛创科技有限公司
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