Antigen-free porcine dermal collagen fibers

A collagen fiber, antigen-free technology, used in fiber processing, chemical post-processing of fibers, animal raw materials, etc., can solve the problems of unstable biocompatibility, poor mechanical properties, and unsuitable pore structure for three-dimensional cell growth. Excellent mechanical properties, controllable conditions, significant ecological and environmental benefits

Active Publication Date: 2011-07-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. There is still the problem that the pore structure is not suitable for the three-dimensional growth of cells in the preparation of tissue engineering scaffold materials by using acellular porcine dermal matrix materials;
[0007] 2. Using the hydrolyzate of pigskin to compound with other natural/syntheti...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Weighing: Weigh the purified pigskin accurately, as the basis for the calculation of the following processes.

[0050] (2) Cleaning: ①. Technical regulations: water 400%; internal temperature: 18°C; penetrant JFC 0.1%. ② Operation method: After adjusting the water volume and internal temperature, add penetrant JFC, rotate for 60 minutes, and control the water.

[0051] (3) Acid pickling: ①. Technical regulations: water 30%; internal temperature: 10℃; formic acid 1.2%, NaCl 6%. ②Operation method: After adjusting the water volume and internal temperature, add NaCl, rotate for 15 minutes, and then add formic acid diluted with 8-15 times room temperature water from the drum shaft hole during rotation, and rotate for 60 minutes. Check the end point of pickling pH=2.2~2.5.

[0052] (4) Enzyme treatment: ①. Technical regulations: Carry out in an acid bath. Compound enzyme skin collagen treatment agent F 0.10%. ②. Operation method: After the acid pickling meets the requirement...

Embodiment 2

[0067] (1) Weighing: Weigh the purified pigskin accurately, as the basis for the calculation of the following processes.

[0068] (2) Cleaning: ①. Technical regulations: water 500%; internal temperature: 20℃; penetrant JFC 0.3%. ②. Operation method: After adjusting the water volume and internal temperature, add penetrant JFC, rotate for 75 minutes, and control the water.

[0069] (3) Acid pickling: ①. Technical regulations: water 30%; internal temperature: 20℃; formic acid 1.5%, NaCl 7.0%. ②. Operation method: After adjusting the water volume and internal temperature, add NaCl, rotate for 45 minutes, and then add formic acid diluted with 8-15 times room temperature water from the drum shaft hole during rotation, and rotate for 80 minutes. Check the end point of pickling pH=2.2~2.5.

[0070] (4) Enzyme treatment: ①. Technical regulations: Carry out in an acid bath. Compound enzyme skin collagen treatment agent F 0.6%. ②Operation method: After the acid pickling meets the requiremen...

Embodiment 3

[0085] (1) Weighing: Weigh the purified pigskin accurately, as the basis for the calculation of the following processes.

[0086] (2) Cleaning: ①. Technical regulations: water 600%; internal temperature: 24℃; penetrant JFC 0.5%. ② Operation method: After adjusting the water volume and internal temperature, add penetrant JFC, rotate for 90 minutes, and control the water.

[0087] (3) Acid pickling: ①. Technical regulations: water 80%; internal temperature: 24℃; formic acid 3.0%, NaCl 10%. ②Operation method: After adjusting the water volume and internal temperature, add NaCl, rotate for 15 minutes, and then add formic acid diluted with 8-15 times room temperature water from the drum shaft hole during rotation, and rotate for 90 minutes. Check the end point of pickling pH=2.2~2.5.

[0088] (4) Enzyme treatment: ①. Technical regulations: Carry out in an acid bath. Composite enzyme skin collagen treatment agent F 0.80%. ②. Operation method: After the acid pickling meets the requiremen...

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PUM

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Abstract

The invention discloses antigen-free porcine dermal collagen fibers and a preparation method thereof. The antigen-free porcine dermal collagen fibers with excellent performance and wide application are prepared by using purified porcine skin as a raw material and adopting a method of combining physics, chemistry and biochemistry to perform a series of treatment such as fluffing, fiber decomposition, fiber separation, carding and the like on the purified porcine skin on the premise of ensuring good mechanical property of the purified porcine skin unchangeable. The antigen-free porcine dermal collagen fibers have good biocompatibility, good mechanical property and proper degradability, and can be widely applied to preparation of medicinal biological materials such as tissue engineering scaffold materials, hemostatic materials, medicinal operation stitches and the like.

Description

Technical field [0001] The invention relates to antigen-free pig dermal collagen fibers and a preparation method thereof, and belongs to the field of biomedical materials. Background technique [0002] So far, as far as the world is concerned, whether it is organ transplantation or tissue defect repair, there are serious shortages of allogeneic organs or tissue donors, and negative effects such as rejection caused by allogeneic organ or tissue transplantation. The rise and rapid development of tissue engineering have brought hope to organ transplantation and repair and reconstruction of soft and hard tissues. Tissue engineering materials, especially the research and development of tissue engineering scaffold materials, have become and will continue to be one of the research hotspots and focuses of international biomaterials. [0003] Collagen has good biocompatibility, weak antigenicity, excellent hydrophilicity and unique porous structure. It is a rare ideal biomaterial. The app...

Claims

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Application Information

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IPC IPC(8): D01C3/00C12S11/00D01B9/00A61L15/40A61L17/08A61L27/36A61L27/60
Inventor 但年华但卫华林海胡杨曾睿肖世维吴霞
Owner SICHUAN UNIV
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