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Application of selective cell-removed pork skin as skin substitute of human body and its preparation method

A human skin and decellularization technology, applied in the field of pigskin, can solve the problems of high cost, easy infection, and high price, and achieve the effects of low price, easy preparation, and wide sources

Inactive Publication Date: 2008-09-03
陕西艾尔肤组织工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disadvantages: First, it needs to be covered and protected with other dressings after being applied to the wound, and second, it is expensive; Integra artificial skin was invented by Burke in 1996 and used in clinical practice and approved by the US FDA
Disadvantages: easy infection after transplantation; keratinocyte membrane is not easy to adhere; expensive

Method used

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  • Application of selective cell-removed pork skin as skin substitute of human body and its preparation method

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Experimental program
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Effect test

Embodiment Construction

[0046] The method for preparing the decellularized pigskin of the present invention as a human skin substitute takes about 50 kg of healthy white domestic pigs without skin diseases, kills them alive, shaves and washes the skin, removes a large full-thickness skin, and cuts it back to 0.3-0.4mm The skin slices were cross-linked with 0.5% glutaraldehyde for 10 minutes, washed 5 times with phosphate-balanced saline, then soaked in IM saline containing 0.25% trypsin at 37°C for 2 hours, and then placed in 0.5% Triton Shake in -100 distilled water at 37°C 100 times / min for 36 hours, wash with balanced phosphoric acid saline for 5 times, and store in a 4°C refrigerator for later use.

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Abstract

The present invention discloses a new application of pig skin which can be substituted for human skin and its preparation method. Said preparation method includes the following steps: killing health living while pig with about 50 kg, shaving hair and cleaning pigskin, skinning and chipping to obtain skin sheet, cross-linking with glutaraldehyde, cleaning with balanced salt solution phosphate, soaking it in IM physiological saline containing trypsase, vibrating with Triton-100, more washing it with balanced salt solution phosphate and storing it in refrigerator with 4 deg.C for stand-by. Said invented product has the functional characteristics of two skin substitutes of Integra artificial skin and ADM, so that it not only can be used as temporary wound surface covering material for protecting surface of wound, but also can be used as permanent substitute for dermis for repairing surface of the wound.

Description

technical field [0001] The present invention relates to the use of pigskin, in particular to the application as a human skin substitute. The invention also relates to a method for preparing pigskin as a human skin substitute. Background technique [0002] At present, for the treatment of skin burn wounds, especially for the treatment of large-scale deep burn wounds, early cutting of scabs and timely sealing of burn wounds are one of the most critical and effective measures to improve the survival rate of burn wounds. One of the best wound coverings is the casualty's autologous skin. However, wounded autologous skin transplantation will not only produce new wounds in the donor area, but also cause scar formation and pigmentation in the donor area due to dermal damage. Therefore, seeking an ideal permanent human skin substitute has become an important topic in burn plastic surgery. [0003] Currently, available human skin substitutes mainly include temporary skin substitute...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60
Inventor 柴家科杨红明刘强
Owner 陕西艾尔肤组织工程有限公司
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