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Method for preparing decellularized dermis material for skin grafting

A technology of acellular dermis and skin transplantation, which is applied in the field of preparation of acellular dermal materials for skin transplantation, can solve the problems of reducing immunogenicity, residual biological toxicity, and destroying cells, and achieve the effect of reducing immunogenicity

Inactive Publication Date: 2015-07-01
BEIJING JISHUITAN HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing acellular dermis manufacturing technologies all use chemical reagents to destroy the cells, and combine glutaraldehyde and other cross-linking agents to cross-link to reduce immunogenicity, which has the disadvantages of destroying the collagen structure and remaining biological toxicity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] The present invention is described in detail below in conjunction with the examples: a preparation method of acellular dermal material for skin transplantation, which adopts the technical scheme of repeated freezing and thawing combined with ultrasonic oscillation method, and the specific process steps are as follows:

[0016] 1. Take the skin of the back of the 3-month-old Zhonghuaxiang pig, wash it, remove the hair with a depilator, and cut it with a skin drum to take a medium-thick skin piece with a thickness of 0.25-0.35 mm for later use;

[0017] 2. Seal and mark the aseptic plastic bags of the skin slices and place them in a -85°C ultra-low temperature refrigerator, freeze them for 7 hours, take them out, rewarm them in warm water at 45°C until the skin slices are completely melted, and repeat the low-temperature freezing and rewarming process 3 times;

[0018] 3. Immerse the skin piece that has been repeatedly frozen and thawed in 4% hypertonic sodium chloride sol...

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Abstract

The invention discloses a method for preparing a decellularized dermis material for skin grafting. According to the decellularized dermis material which is prepared from process steps of repeatedly freezing and thawing pig skin with the combination of ultrasonic oscillation, cell components in dermis are sufficiently removed through a physical method, the immunogenicity of the dermis is reduced without affecting the three-dimensional structure and arrangement polarity of collagenous fiber, complete basilar membrane can be maintained to be used as a complete tissue frame and a template for dermis regeneration, and the natural three-dimensional interspace of the decellularized dermis is beneficial for growth of fibroblast and vascular endothelial cells and reconstruction of matrix, so that the decellularized dermis material can be used as good tissue engineering dermis to be applied to clinical application.

Description

Technical field [0001] The invention relates to the field of medical materials for clinical application in medical burns and plastic surgery, in particular to a method for preparing acellular dermis materials for skin transplantation. Background technique [0002] At present, in the clinical practice of medical burns and plastic surgery, there are huge wounds with skin loss in patients with severe trauma and extensive burns, and autologous skin transplantation is basically the main way to eliminate wounds in treatment, but the source of autologous skin is tight, especially for large areas. It is even more difficult or unrealistic to obtain autologous skin for skin defects. Therefore, during the treatment process, skin substitutes are needed to cover the wound temporarily or for a long time. Finding skin substitutes to cover the wound has become the first choice for the treatment of severe trauma and extensive burns. need. The current skin dermis substitutes, whose sources i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/24A61L27/60
Inventor 张国安宁方刚赵筱卓
Owner BEIJING JISHUITAN HOSPITAL
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