The invention discloses a urine separation and collection device, which comprises a urine collection pool arranged on a drain pipe on the bottom of a urinal, wherein an alarming device is arranged in the urine collection pool, the side face of the urine collection pool is connected with the drain pipe through an overflow pipe, a small-flow liquid guide pipe is arranged above the urine collection pool, a valve is arranged on the small-flow liquid guide pipe, and the small-flow liquid guide pipe is further arranged on the drain pipe; a connecting funnel is arranged on the inlet end of the small-flow liquid guide pipe, a bundling funnel is arranged above the collecting funnel, and a filter screen is arranged avove the bundling funnel. The urine separation and collection device has the advantages of simple structure, convenience in assembly, simplicity in maintenance, long service life, and greatly lower production cost compared with current similar products; and the device is environment-friendly and hygienic, the problems of strong odor, poor environmental and hygienic condition and the like caused by a primitive urine collection method are prevented, the separate and sealing type collection of urine enables pollution of flushing water to be low, and subsequent purification treatment and circulated recycling are facilitated.