Antibacterial peptide activity detection kit and detection method

Abstract

The invention discloses an agar solution, which comprises agar and alkali, wherein the concentration of agar is 2-3%(w/v), the concentration of alkali is no less than 0.8mol/L. The invention also provides a detection kit including the above mentioned agar solution and a detection method of the kit. The kit is employed for detecting the antibacterial peptide activity, the detection result is accurate and reliable, and the kit has low requirement for the experiment condition, the operation is simple, ordinary people can use the kit, and the practicality is strong.

Description

technical field [0001] The invention relates to an antimicrobial peptide activity detection kit and a detection method. Background technique [0002] The detection of antimicrobial peptide activity usually adopts the tube-and-disk method. The tube-and-disk method is based on the diffusion and penetration of antibiotics in the agar plate medium, and compares the size of the inhibition zone of the standard product and the test product against the indicator bacteria to determine the test product. valence. The basic principle of the tube-and-dish method is to place an Oxford cup on an agar plate containing highly sensitive test bacteria, put the solution of the standard product and the product to be tested in the cup, and cultivate it at a constant temperature for 16 to 18 hours. The area producing transparent sterile growth, often circular, is called the zone of inhibition. The diameter of the inhibition zone is related to the potency of the antibiotic, and the potency of the...

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Problems solved by technology

However, it is difficult to sterilize effectively for those who have poor test conditions or have no test operation ability at all. At the same time, because the gel solution forms a gel when the temperature is lowered below 40°C, the optimum temperature for the growth and reproduction of most bacteria is usually 20-40°C, if the indicator bacteria are added below 40°C, the indicator bacteria and the gel-like agar cannot be mixed evenly, resulting in uneven distribution of the indicator bacteria, resulting in inaccurate test results. If the indicator bacteria are added above 40°C, It will make it difficult for the indicator bacteria to grow effectively, reduce the detection efficiency or cause the detection to fail

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