Preparing method and application of composite biological agent for agricultural root-knot nematodes

A root-knot nematode and biocontrol fungicide technology, applied in the field of agricultural microorganisms, can solve problems such as crop quality decline, and achieve the effects of promoting crop growth, inhibiting plant pathogens, and facilitating cultivation

Active Publication Date: 2016-02-10
LUDONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At the same time, the extensive use of chemical pesticides has caused the

Method used

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  • Preparing method and application of composite biological agent for agricultural root-knot nematodes
  • Preparing method and application of composite biological agent for agricultural root-knot nematodes

Examples

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Example Embodiment

[0029] Example 1 Preparation of agricultural root-knot nematode composite biocontrol agent by shallow dish culture

[0030] Pick 1 loop from the preserved bacterial species on the slope of the test tube and inoculate it in the activation medium, culture it at 30°C and 200 rpm for 36 hours until the bacterial cell becomes turbid, to obtain the activation seed liquid; the activation medium (g / L): peptone 10. Yeast powder 5, glycerol 25, powdered chitosan 2, potassium dihydrogen phosphate 3, magnesium sulfate 3, disodium hydrogen phosphate 3, pH 6.5.

[0031] The solid fermentation medium used: 30% of dry beer lees, 8% of bran, 6% of ground peanut shells, 5% of soy peptone, powdered chitosan (with a degree of deacetylation above 90%) 7.5%, crab shell powder 2.25 %, NaCl0.5%, CaCl 2 0.3%, MgSO 4 0.3%, FeSO 4 0.15%, adding purified water to a water content of 40% based on the total mass of the medium components. Preparation method: first add NaCl, CaCl with water 2 , MgSO 4 , FeSO 4 , ...

Example Embodiment

[0034] Example 2 Preparation of agricultural root-knot nematode composite biocontrol agent in solid fermentation tank

[0035] Pick a loop of bacteria from the slant of the test tube and inoculate it in the activation medium, and incubate at 28°C and 200 rpm for 48 hours until the cells become turbid to prepare the activated seed liquid; the activation medium (g / L): peptone 5. Yeast powder 3, glycerol 20, powdered chitosan 1, potassium dihydrogen phosphate 2, magnesium sulfate 2, disodium hydrogen phosphate 2, pH 6.5.

[0036] The solid fermentation medium used: 17% of dry beer lees, 4% of bran, 3% of ground peanut shells, 2% of soy peptone, 2.55% of powdered chitosan (degree of deacetylation above 90%), and 1 crab shell powder %, NaCl0.2%, CaCl 2 0.1%, MgSO 4 0.1%, FeSO 4 0.055%, add purified water to 70% water content based on the total mass of the medium components. Preparation method: first add NaCl, CaCl with water 2 , MgSO 4 , FeSO 4 , MnSO 4 Dissolve with soy peptone, stir ...

Example Embodiment

[0039] Example 3 Preparation of agricultural root-knot nematode composite biocontrol agent in a ventilated fermentation tank

[0040] Pick a loop of the strains preserved on the slant of the test tube and inoculate it in the activation medium, culture it at 28~30℃, 200rpm for 36~48h until the bacteria are turbid, to prepare the activation seed liquid; the activation medium (g / L): Peptone 8, yeast powder 4, glycerol 22, powdered chitosan 2, potassium dihydrogen phosphate 2, magnesium sulfate 2, disodium hydrogen phosphate 3, pH 6.5.

[0041] The solid fermentation medium used: 30% of dry beer lees, 6% of bran, 5% of ground peanut shells, 5% of soy peptone, 6% of powdered chitosan (with a degree of deacetylation above 90%), and crab shell powder 2 %, NaCl0.5%, CaCl 2 0.3%, MgSO 4 0.3%, FeSO 4 0.15%, add tap water to a water content of 44.75% based on the total mass of the medium components. Preparation method: first add NaCl, CaCl with water 2 , MgSO 4 , FeSO 4 , MnSO 4 Dissolve wi...

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Abstract

The invention relates to a preparing method and application of a composite biological agent for agricultural root-knot nematodes. The biological agent is obtained through solid fermentation of paecilomyces lilacinus YT08 with the preservation number of CG MCC No.10026 and has a powerful inhibitory effect on the agricultural root-knot nematode disease. A preferred culture medium involved in solid fermentation is prepared from 17-30% of dried brewer grain, 4-8% of bran, 3-6% of smashed peanut shells, 2-5% of soy peptone, 2.55-7.5% of powder chitosan (with the deacetylation degree larger than or equal to 90%), 1-2.25% of crab shell meal, 0.2-0.5% of NaCl, 0.1-0.3% of CaCl2, 0.1-0.3% of MgSO4, 0.05-0.15% of FeSO4 and 40-70% of water. The preparing method has the advantages of being easy to operate and control and suitable for industrial production, the raw materials are low in cost, and the composite biological agent has the powerful effect.

Description

technical field [0001] The invention relates to a preparation method of a compound biocontrol agent for agricultural root-knot nematode and its application in the biological control of agricultural root-knot nematode, belonging to the technical field of agricultural microorganisms. Background technique [0002] Plant diseases and insect pests are an important reason for the reduction of crop production. The annual loss due to the reduction of plant diseases and insect pests is huge. The growers use a large amount of chemical pesticides to reduce the occurrence of plant diseases and insect pests, which in turn increases the resistance of pathogenic organisms to chemical drugs, forming a vicious circle. At the same time, the large-scale use of chemical pesticides has caused the quality of crops to decline, and many people talk about food discoloration. In this context, high-efficiency and low-toxic biopesticides have gradually attracted the attention of farmers, production ent...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/79
Inventor 程仕伟解孝满张萍孙虎山刘丹解卫海陈国忠
Owner LUDONG UNIVERSITY
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