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Preparing method and application of composite biological agent for agricultural root-knot nematodes

A root-knot nematode and biocontrol fungicide technology, applied in the field of agricultural microorganisms, can solve problems such as crop quality decline, and achieve the effects of promoting crop growth, inhibiting plant pathogens, and facilitating cultivation

Active Publication Date: 2016-02-10
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the extensive use of chemical pesticides has caused the quality of crops to decline, and many people talk about food discoloration.

Method used

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  • Preparing method and application of composite biological agent for agricultural root-knot nematodes
  • Preparing method and application of composite biological agent for agricultural root-knot nematodes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 shallow plate culture prepares agricultural root-knot nematode compound biocontrol agent

[0030] Pick 1 ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 30°C and 200rpm for 36 hours until the bacteria are turbid to obtain the activated seed liquid; the activation medium (g / L): peptone 10. Yeast powder 5, glycerin 25, chitosan powder 2, potassium dihydrogen phosphate 3, magnesium sulfate 3, disodium hydrogen phosphate 3, pH 6.5.

[0031] Solid fermentation medium used: 30% dry brewer’s grains, 8% bran, 6% crushed peanut shells, 5% soybean peptone, 7.5% powdered chitosan (deacetylation degree above 90%), 2.25% crab shell powder %, NaCl0.5%, CaCl 2 0.3%, MgSO 4 0.3%, FeSO 4 0.15%, according to the conversion of the total mass of the medium components, add purified water to reach a water content of 40%. Preparation method: first dissolve NaCl and CaCl with water 2 , MgSO 4 , FeSO ...

Embodiment 2

[0034] Embodiment 2 solid fermenter prepares agricultural root-knot nematode composite biocontrol agent

[0035] Pick 1 ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 28°C and 200rpm for 48h until the bacteria are turbid to obtain the activated seed solution; the activation medium (g / L): peptone 5. Yeast powder 3, glycerin 20, chitosan powder 1, potassium dihydrogen phosphate 2, magnesium sulfate 2, disodium hydrogen phosphate 2, pH 6.5.

[0036] The solid fermentation medium used: 17% dry brewer's grains, 4% bran, 3% crushed peanut shells, 2% soybean peptone, 2.55% powdered chitosan (deacetylation degree above 90%), and 1% crab shell powder %, NaCl0.2%, CaCl 2 0.1%, MgSO 4 0.1%, FeSO 4 0.055%, according to the conversion of the total mass of the medium components, add purified water to reach a water content of 70%. Preparation method: first dissolve NaCl and CaCl with water 2 , MgSO 4 , FeSO 4 ...

Embodiment 3

[0039] Embodiment 3 ventilated fermenter prepares agricultural root-knot nematode composite biocontrol agent

[0040] Pick 1 ring from the preserved strains on the slant of the test tube and inoculate it in the activated medium, and cultivate it at 28~30°C and 200rpm for 36~48h until the bacteria are turbid to obtain the activated seed liquid; the activated medium (g / L): peptone 8, yeast powder 4, glycerin 22, chitosan powder 2, potassium dihydrogen phosphate 2, magnesium sulfate 2, disodium hydrogen phosphate 3, pH 6.5.

[0041] Solid fermentation medium used: 30% dry brewer’s grains, 6% bran, 5% crushed peanut shells, 5% soybean peptone, 6% powdered chitosan (deacetylation degree above 90%), 2% crab shell powder %, NaCl0.5%, CaCl 2 0.3%, MgSO 4 0.3%, FeSO 4 0.15%, according to the conversion of the total mass of the medium components, add tap water to a water content of 44.75%. Preparation method: first dissolve NaCl and CaCl with water 2 , MgSO 4 , FeSO 4 , MnSO 4 ...

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Abstract

The invention relates to a preparing method and application of a composite biological agent for agricultural root-knot nematodes. The biological agent is obtained through solid fermentation of paecilomyces lilacinus YT08 with the preservation number of CG MCC No.10026 and has a powerful inhibitory effect on the agricultural root-knot nematode disease. A preferred culture medium involved in solid fermentation is prepared from 17-30% of dried brewer grain, 4-8% of bran, 3-6% of smashed peanut shells, 2-5% of soy peptone, 2.55-7.5% of powder chitosan (with the deacetylation degree larger than or equal to 90%), 1-2.25% of crab shell meal, 0.2-0.5% of NaCl, 0.1-0.3% of CaCl2, 0.1-0.3% of MgSO4, 0.05-0.15% of FeSO4 and 40-70% of water. The preparing method has the advantages of being easy to operate and control and suitable for industrial production, the raw materials are low in cost, and the composite biological agent has the powerful effect.

Description

technical field [0001] The invention relates to a preparation method of a compound biocontrol agent for agricultural root-knot nematode and its application in the biological control of agricultural root-knot nematode, belonging to the technical field of agricultural microorganisms. Background technique [0002] Plant diseases and insect pests are an important reason for the reduction of crop production. The annual loss due to the reduction of plant diseases and insect pests is huge. The growers use a large amount of chemical pesticides to reduce the occurrence of plant diseases and insect pests, which in turn increases the resistance of pathogenic organisms to chemical drugs, forming a vicious circle. At the same time, the large-scale use of chemical pesticides has caused the quality of crops to decline, and many people talk about food discoloration. In this context, high-efficiency and low-toxic biopesticides have gradually attracted the attention of farmers, production ent...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/79
Inventor 程仕伟解孝满张萍孙虎山刘丹解卫海陈国忠
Owner LUDONG UNIVERSITY
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