The invention discloses bacillus wettable powder containing brassinolide and a preparation method thereof. The bacillus wettable powder containing brassinolide is mainly prepared from brassinolide, a bacillus solution, nonyl phenol polyoxyethylene ether, ethanol, skim milk powder, methylcellulose, D425, sodium dodecyl benzene sulfonate and kieselguhr. The brassinolide is capable of improving the activity of plant based enzymes, improving the seed vigor, promoting early development, enhancing the photosynthetic efficiency, increasing the crop yield, improving the crop quality, improving the cold resistance, drought resistance, waterlogging resistance and saline-alkaline tolerance of crops, improving the stress tolerance, and alleviating pesticide phytotoxicity and chemical pollution phytotoxicity, and is capable of resisting hazards of multiple types of virus, bacteria and fungus and enhancing the crop disease resistance. The bacillus is capable of directly restraining or killing multiple types of plant diseases. The wettable powder is safe, environmentally friendly and efficient, is stable in production process, and can be widely applied to crops such as rice, flue-cured tobacco, corn, wheat, stawberries, watermelons, hot pepper, tomatoes and cucumbers.