Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide

A technology of Bacillus subtilis and bacterial agents, applied in the field of microorganisms, can solve the problems of reducing quality and storage period, affecting the appearance of potatoes, reducing the quality of potato pieces, etc., achieving the effect of prolonging the storage period, shortening the period and maintaining ecological balance

Active Publication Date: 2016-05-18
山东京青农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, according to the different types of pathogenic bacteria, it often appears as raised or sunken surface lesions on mature potato pieces. In severe cases, the lesions are connected and the quality of potato pieces is

Method used

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  • Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide
  • Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide
  • Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1: Isolation and screening of Bacillus subtilis JQ-001 strain:

[0032] 1. Separation

[0033] (1) Soil collection: 100g soil samples were collected from the surface of the soil in the rice field of Jiansanjiang Farm in Heilongjiang for more than 5 years to 10cm, and 5 samples were collected in parallel at one location and mixed through a 40-mesh grading sieve. Take 100g of the soil under the sieve for separation.

[0034] (2) Medium preparation: separate preparation

[0035] NA medium: peptone 10g / L, beef extract 3.0g / L, sodium chloride 5g / L, agar 18g / L, pH=7.0-7.2;

[0036] And PDA medium: 200g potato, 20g glucose, 20g agar powder, 1000mL distilled water;

[0037] Make medium plates.

[0038] (3) Accurately weigh 1g of soil and place it in a 250ml Erlenmeyer flask, add 99ml of sterile water, shake at 200rpm at 30-37℃ for 30min. Make a soil suspension and dilute it to 10 -8 . Separate single colonies in NA medium and PDA medium, and separate single colonies were activat...

Example Embodiment

[0049] Example 2: Identification of Bacillus subtilis JQ-001 strain

[0050] 1. Microbiological characteristics: The strain of JQ-001 strain is short rod-shaped, 0.6-0.7μm×1.9-3.0μm, arranged individually or in pairs, columnar spores, mesophytic, slightly enlarged cysts, Gram-positive. The strain was cultured on NA medium at 37°C for 24h, the colony was milky white, the surface was convex, the edges were not neat, and it was dry and opaque.

[0051] 2. Physiological and biochemical characteristics:

[0052] Table 3 Physiological and biochemical characteristics of Bacillus subtilis JQ-001 strain

[0053] Test items

[0054] Note: "+" means positive reaction, "-" means negative reaction

[0055] 3. Molecular biology identification:

[0056] The 16S rDNA amplified sequence of the JQ-001 strain was compared in the NCBI database, and the homology with Bacillus subtilis was 99.38%. Combined with the morphological and physiological and biochemical characteristics of the JQ-001 strain, it w...

Example Embodiment

[0057] Example 3: Preparation of Bacillus subtilis liquid inoculum

[0058] (1) Strain activation: inoculate the Bacillus subtilis JQ-001 strain stored at low temperature on LB solid medium and culture it at 36℃ for 36h; pick a single colony and transfer it to LB slant medium and cultivate it at 36℃ for 20h , Wash the surface of the culture medium with sterile water as the inoculum.

[0059] (2) Preparation of the first-level seed solution: add the inoculum prepared in step (1) to the seed culture medium at an inoculum amount of 10% (volume percentage), rotate at 220 rpm, at a temperature of 36°C, and incubate on a shaker for 20 hours as the first level Seed liquid.

[0060] (3) Preparation of secondary seed liquid: Inoculate the seed culture medium with the primary seed liquid prepared in step (2) according to the inoculum of 3% (volume percentage), the aeration volume is 1.0 vvm, the stirring speed is 220 rpm, and the cultivation time For 20h. As a secondary seed liquid.

[0061]...

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Abstract

The invention discloses bacillus subtilis, bactericide, a preparing method of the bactericide and application of the bactericide. The bacillus subtilis is bacillus subtilis JQ-001 and is preserved in the common microorganism center of China microorganism strain preservation management committee, preservation time is November 9th, 2015, and the preservation register number is CGMCCNo.11632; the liquid bactericide or the solid bactericide can be prepared by means of seed activation, seed culture and fermentation culture. The invention further provides a preparing method of the bacillus subtilis, and application to preparation of medicine for preventing and treating rice blast and potato scab. The bactericide has high bioactivity and has the advantages of being short in production period and low in production cost; meanwhile, environment compatibility of the bactericide is good, the effects of preventing and treating rice blast and potato scab are remarkable, no hazardous effect is generated for people and livestock, and use is convenient.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis, a bacterial agent, a preparation method of the bacterial agent and an application of the bacterial agent. Background technique [0002] Rice blast is one of the important diseases of rice. It occurs in all rice regions in the world, and most of them occur on the leaves and nodes. After the occurrence, it can cause different degrees of production reduction. In severe cases, the production reduction is 40%-50%, causing heavy losses. The disease is caused by Pyrospora oryzae. The fungus overwinters on rice straw and rice with conidia and mycelium. The conidia produced in the next year are spread to rice plants by wind and rain. They germinate and invade the host to spread to adjacent cells, forming a central disease. strain. The conidia formed in the diseased part are spread by wind and rain to infect again, and sowing infected seeds can cause seedling b...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
CPCA01N63/10C12N1/20C12N1/205C12R2001/125
Inventor 史盼盼李元鑫郭婧贾振宇
Owner 山东京青农业科技有限公司
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