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Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide

A technology of Bacillus subtilis and bacterial agents, applied in the field of microorganisms, can solve the problems of reducing quality and storage period, affecting the appearance of potatoes, reducing the quality of potato pieces, etc., achieving the effect of prolonging the storage period, shortening the period and maintaining ecological balance

Active Publication Date: 2016-05-18
山东京青农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, according to the different types of pathogenic bacteria, it often appears as raised or sunken surface lesions on mature potato pieces. In severe cases, the lesions are connected and the quality of potato pieces is reduced.
At the same time, since the epidermal tissue is destroyed, it is easy to be infected by other pathogenic bacteria, causing tuber rot, seriously affecting the appearance of potatoes, reducing their quality and storage period

Method used

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  • Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide
  • Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide
  • Bacillus subtilis, bactericide, preparing method of bactericide and application of bactericide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Isolation and screening of Bacillus subtilis JQ-001 bacterial strain:

[0032] 1. Separation

[0033] (1) Soil collection: 100g of soil samples were collected from the paddy field of Jiansanjiang Farm, Heilongjiang, which had been continuously cropped for more than 5 years, from the soil surface to 10cm. Five samples were collected in parallel at one location, and mixed through a 40-mesh grading sieve. Take 100g of sieved soil for separation.

[0034] (2) Medium preparation: prepared separately

[0035] NA medium: peptone 10g / L, beef extract 3.0g / L, sodium chloride 5g / L, agar 18g / LpH=7.0-7.2;

[0036] And PDA medium: potato 200g, glucose 20g, agar powder 20g, distilled water 1000mL;

[0037] Make media plates.

[0038] (3) Accurately weigh 1 g of soil and place it in a 250 ml Erlenmeyer flask, add 99 ml of sterile water, and shake on a shaker at 200 rpm at 30-37°C for 30 min. Make a soil suspension and dilute to 10 by a 10-fold gradient -8 . Single ...

Embodiment 2

[0049] Embodiment two: the identification of Bacillus subtilis JQ-001 bacterial strain

[0050] 1. Microbiological characteristics: JQ-001 strain is short rod-shaped, 0.6-0.7μm×1.9-3.0μm, arranged singly or in pairs, spores are columnar, mesogenic, cysts are slightly enlarged, and Gram-positive. The strain was cultured in NA medium at 37°C for 24 hours, and the colonies were milky white, with raised surfaces, irregular edges, and dry and opaque.

[0051] 2. Physiological and biochemical characteristics:

[0052] Table 3 Physiological and biochemical characteristics of Bacillus subtilis JQ-001 strain

[0053] Test items

characteristics

Test items

characteristics

Test items

characteristics

7% Nacl growth

+

arginine dihydrolase

-

Mannitol

+

Grow at 15°C

+

β-galactase

-

starch hydrolysis

+

Growth at 50°C

+

H2S production

-

Citric acid utilization

+

D-glucose

...

Embodiment 3

[0057] Embodiment three: the preparation of bacillus subtilis liquid bacterial agent

[0058] (1) Strain activation: inoculate the low-temperature preserved Bacillus subtilis JQ-001 strain on LB solid medium, and culture at 36°C for 36h; pick a single colony and transfer it to LB slant medium, and culture at 36°C for 20h , wash the bacteria on the surface of the culture medium with sterile water as the inoculum.

[0059] (2) Preparation of first-level seed solution: add the inoculum solution prepared in step (1) to the seed medium according to the inoculation amount of 10% (volume percentage), at a rotating speed of 220 rpm, at a temperature of 36° C., and culture on a shaking table for 20 h, as a first-level seed liquid.

[0060] (3) Secondary seed liquid preparation: insert the primary seed liquid prepared by step (2) into the seed culture medium according to the inoculum size of 3% (volume percentage), the ventilation rate is 1.0vvm, the stirring speed is 220rpm, and the c...

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Abstract

The invention discloses bacillus subtilis, bactericide, a preparing method of the bactericide and application of the bactericide. The bacillus subtilis is bacillus subtilis JQ-001 and is preserved in the common microorganism center of China microorganism strain preservation management committee, preservation time is November 9th, 2015, and the preservation register number is CGMCCNo.11632; the liquid bactericide or the solid bactericide can be prepared by means of seed activation, seed culture and fermentation culture. The invention further provides a preparing method of the bacillus subtilis, and application to preparation of medicine for preventing and treating rice blast and potato scab. The bactericide has high bioactivity and has the advantages of being short in production period and low in production cost; meanwhile, environment compatibility of the bactericide is good, the effects of preventing and treating rice blast and potato scab are remarkable, no hazardous effect is generated for people and livestock, and use is convenient.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis, a bacterial agent, a preparation method of the bacterial agent and an application of the bacterial agent. Background technique [0002] Rice blast is one of the important diseases of rice. It occurs in all rice regions in the world, and most of them occur on the leaves and nodes. After the occurrence, it can cause different degrees of production reduction. In severe cases, the production reduction is 40%-50%, causing heavy losses. The disease is caused by Pyrospora oryzae. The fungus overwinters on rice straw and rice with conidia and mycelium. The conidia produced in the next year are spread to rice plants by wind and rain. They germinate and invade the host to spread to adjacent cells, forming a central disease. strain. The conidia formed in the diseased part are spread by wind and rain to infect again, and sowing infected seeds can cause seedling b...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
CPCA01N63/10C12N1/20C12N1/205C12R2001/125
Inventor 史盼盼李元鑫郭婧贾振宇
Owner 山东京青农业科技有限公司
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