Efficient phosphorus-dissolving growth-promoting bacterium and bio-fertilizer prepared from same as well as application of efficient phosphorus-dissolving growth-promoting bacterium

A technology of biological fertilizer and compound microbial agent, applied in the field of microbiology, can solve the problems of phosphorus deficiency, low utilization rate of phosphorus fertilizer, and decreased production potential, and achieve the effects of efficient utilization and saving of phosphorus fertilizer, promotion of crop growth, and enhancement of effectiveness

Inactive Publication Date: 2016-03-30
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a widely adaptable high-efficiency phosphorus-dissolving growth-promoting bacterium, a microbial agent prepared therefrom, a biol

Method used

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  • Efficient phosphorus-dissolving growth-promoting bacterium and bio-fertilizer prepared from same as well as application of efficient phosphorus-dissolving growth-promoting bacterium
  • Efficient phosphorus-dissolving growth-promoting bacterium and bio-fertilizer prepared from same as well as application of efficient phosphorus-dissolving growth-promoting bacterium
  • Efficient phosphorus-dissolving growth-promoting bacterium and bio-fertilizer prepared from same as well as application of efficient phosphorus-dissolving growth-promoting bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation and cultivation of embodiment 1 Aspergillus niger A6

[0027] A strain of fungus A6 with a strong ability to dissolve insoluble phosphorus in soil was screened from the farmland soil (calcareous soil) of Shijiazhuang City, Hebei Province, my country. Based on the observation of the morphological characteristics of the strain and the results of molecular biology sequencing, the strain A6 was finally determined to be black Aspergillus niger.

[0028] (1) Aspergillus niger A6 medium

[0029] Fermentation broth formula (kg / t fermentation broth): 0.1kg dipotassium hydrogen phosphate, 0.1kg potassium dihydrogen phosphate, 4.3kg sodium chloride, 0.1kg magnesium sulfate, 8.6kg starch, 4.3kg beef extract, 4.3kg peptone, yeast Powder 4.3kg, sucrose 8.6kg, ammonium sulfate 2.6kg, calcium carbonate 10kg, ferrous sulfate 0.001kg, soybean oil 6.8kg, prepared with water.

[0030](2) Cultivation of Aspergillus niger A6

[0031] Inoculate the Aspergillus niger A6 strain on b...

Embodiment 2

[0033] The preparation of embodiment 2 high-efficiency phosphorus-dissolving and growth-promoting microbial bacterial agents

[0034] 1. Cultivation of Aspergillus niger A6

[0035] (1) Composition of medium (kg / t fermentation liquid)

[0036] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg,K 2 HPO 4 0.2kg, NaCl2.5kg, MgSO 4 ·7H 2 O0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH7.0, prepared with water.

[0037] (2) Fermentation culture

[0038] Aspergillus niger A6 was inoculated on potato sucrose (PDA) medium and cultured at 25-28°C for 3 days. Transfer the spores of Aspergillus niger A6 into a 1000mL Erlenmeyer flask, and conduct liquid fermentation at 26-28°C and 220r / min for 36h. Then insert 50L seed tank by 1v / v% inoculum amount, under 200r / min, pH7.0, ventilation rate 0.7vvm condition, cultivate after 36h, then transfer to 500L fermenter by 10v / v% inoculum amount, at 2...

Embodiment 3

[0043] Embodiment 3 Aspergillus niger A6 dissolves the effect of insoluble phosphorus on solid plate

[0044] Experimental object: phosphorus-dissolving growth-promoting bacteria-Aspergillus niger A6, and Bacillus megaterium (ATCC14581) and Penicillium bayley (ATCC20851) were used as controls.

[0045] Under indoor conditions, bone meal (bonemeal), iron phosphate FePO 4 , calcium hydrogen phosphate CaHPO 4 , tricalcium phosphate Ca 3 (PO 4 ) 2 , octacalcium phosphate Ca 8 h 2 (PO 4 ) 6 , Moroccan phosphate rock powder (MRP), lecithin (lecithin), aluminum phosphate AlPO 4 , calcium fluorophosphate Ca 5 (PO 4 ) 3 F is poorly soluble phosphorus, cultured with Pikavskaya solid medium for 5 days, and the phosphorus-dissolving ability of each strain was determined.

[0046] The measurement results of the colonies of phosphate-dissolving bacteria and the diameter of the phosphorus-dissolving circle showed (Table 1), A6 in bone meal, FePO 4 , CaHPO 4 , Ca 3 (PO 4 ) 2 ...

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Abstract

The invention discloses an efficient phosphorus-dissolving growth-promoting bacterium (Aspergillus niger) A6 screened from limy soil in northern China and provides a microbial agent and a bio-fertilizer preparation which are prepared from the strain A6. The microbial agent is capable of efficiently dissolving powdered rock phosphate, and the dissolving rate after 5 days reaches 80.4% and exceeds that of phosphorus-dissolving fungi strains reported at present. According to the A6 microbial agent, the validity that phosphorus is difficultly dissolved in soil is remarkably improved, and effective phosphorus in soil is increased by 8.13mu g/g-8.95mu g/g; compared with contrast, the phosphorus absorbing quantity of crops is increased by 82.01%; the corn yield is increased by 15.85%. The efficient phosphorus-dissolving growth-promoting bacterium is significant for the saving of phosphorus fertilizer resources, the sustainable yield increase of grains and the protection of farmland ecological environments.

Description

technical field [0001] The invention belongs to the field of microbiology, and in particular relates to a high-efficiency phosphorus-dissolving and growth-promoting bacterium, a biological fertilizer preparation prepared therefrom and its application. Background technique [0002] Phosphorus is the second most important element for plant growth after nitrogen, but its availability is very low due to soil fixation, making it difficult to meet the needs of crop growth (Wild, 1988). Studies have shown that more than 90% of the phosphorus fertilizer applied to the soil is converted into insoluble phosphorus, acidic soil is converted into iron phosphate and aluminum phosphate, and calcareous soil is converted into calcium phosphate (Hagin and Hadas, 1962; NagelschmidtG, et al. 1994). In order to increase crop yield, the amount of phosphate fertilizer has increased sharply, and phosphate rock resources are facing a crisis of depletion. It is estimated that the world's phosphate r...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F11/08C05G3/04C12R1/685C05G3/80
Inventor 范丙全
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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