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Microorganic fertilizer against various bacteria and its preparation and use

A technology of microbial bacterial fertilizer and multi-antibiotics, applied in biochemical equipment and methods, botanical equipment and methods, microorganisms, etc., can solve the problem that the quick effect of microbial fertilizers is not as good as that of chemical fertilizers, the soil-borne diseases are serious, and the second-generation microbial fertilizers are affected. Development and other issues, to achieve strong inhibitory effect, promote crop growth, and reduce soil-borne diseases.

Inactive Publication Date: 2004-04-21
SOIL & FERTILIZER INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The second-generation microbial fertilizer plays an important role in improving the ecological environment and improving biological security, but its function of simply providing plant nutrients overlaps with that of chemical fertilizers, and the quick-acting performance of microbial fertilizers is not as good as that of chemical fertilizers. Further Development of Second Generation Microbial Fertilizer
[0006] In addition, crop continuous cropping is extremely common in agricultural production, and the resulting soil-borne diseases are very serious, especially in protected cultivation, and these problems cannot be overcome by the application of chemical fertilizers or second-generation microbial fertilizers

Method used

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  • Microorganic fertilizer against various bacteria and its preparation and use
  • Microorganic fertilizer against various bacteria and its preparation and use
  • Microorganic fertilizer against various bacteria and its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 jelly-like bacillus fermentation

[0027] 1) Medium: pH: 7.0-7.2

[0028] For slope and shaker: sucrose: 10g; K 2 HPO 4 : 0.5g; MgSO 4 ·7H 2 O: 0.2g; yeast extract: 0.4g; CaCO 3 : 1g; FeCl 3 : 0.005g; add agar for solid culture: 15-20g.

[0029] For fermenter: 0.1% sucrose, 5% corn flour, 1% soybean cake flour, K 2 HPO 4 : 0.05%, MgSO 4 ·7H 2 O: 0.02%, yeast extract 0.04%, CaCO 3 : 0.1%, FeCl 3 : 0.0005%

[0030] 2) Cultivation and fermentation process

[0031] For slant culture: culture at 28°C±2°C for 36-48 hours.

[0032] Erlenmeyer flask liquid shaker culture: 28°C±2°C, cultivate for 36-48 hours, 200 rpm;

[0033] Fermentation in a first-stage fermenter: 28°C±2°C, culture for 36-48 hours, with aeration between 0.1-1 (volume ratio of aeration per minute to fermentation broth).

[0034] Secondary fermenter fermentation: 28°C±2°C, cultivate for 36-48 hours, and the ventilation rate is between 0.1-1 (the volume ratio of the ventilation rate ...

Embodiment 2

[0037] Embodiment 2 Bacillus licheniformis fermentation

[0038] 1) Medium: g / 1000ml distilled water pH: 7.0

[0039] Medium for slant and shake flask: peptone: 5g; NaCl: 5g; beef extract: 3g; additional agar for solid culture: 15-20g (plus for solid culture)

[0040] Medium for fermenter soybean meal 2%, corn flour 10%, K 2 HPO 4 : 0.05%, MgSO 4 ·7H 2 O: 0.02%, yeast extract 0.04%, NaCl: 0.5%,

[0041] 2) Cultivation and fermentation process

[0042] Incline cultivation: 28°C±2°C, cultivate for 36-48 hours.

[0043] Erlenmeyer flask liquid shaker culture: 28°C±2°C, cultivate for 36-48 hours, 200 rpm.

[0044] Fermentation in a first-stage fermenter: 28°C±2°C, culture for 36-48 hours, with aeration between 0.1-1 (volume ratio of aeration per minute to fermentation broth).

[0045] Secondary fermenter fermentation: 28°C±2°C, cultivate for 36-48 hours, and the ventilation rate is between 0.1-1 (the volume ratio of the ventilation rate per minute to the fermentation broth...

Embodiment 3

[0048] Embodiment 3 Jingyang streptomyces fermentation

[0049] 1) Medium: g / 1000ml distilled water pH: 7.2-7.4

[0050] Inclined and preserved with KNO 3 : 1g; NaCl: 0.5g; MgSO 4 ·7H 2 O: 0.5g; K 2 HPO 4 : 0.5g; FeSO 4 ·7H 2 O: 0.01g; soluble starch: 20g; agar: 20g.

[0051] Bran 80% for solid fermentation, rice straw powder 19%, KH 2 PO 4: 0.5%, (NH 4 ) 2 SO 4 : 0.5%, CaCl 2 0.2%

[0052] Material: water = 1: 2.2

[0053] 2) Cultivation and fermentation process

[0054] Incline cultivation: 22°C±2°C. 48 hours

[0055] Erlenmeyer flask solid culture: 22℃±2℃. 48 hours

[0056] Solid fermentation culture: 22℃±2℃. 48 hours

[0057] The resulting culture is hereinafter referred to as bacterial agent 3.

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Abstract

A multi-resistant microbial fertilizer is prepared from jelly-like bacillus, Bacillus licheniformis, Jingyang streptomycete and trichoderma through determining the proper culture and fermenting conditions, slant culturing, Erlenmeyer flask culturing, fermenting and / or solid culturing, proportional mixing and adsorbing by carrier. Its application method is also disclosed.

Description

Technical field: [0001] The present invention relates to microbial fertilizer and its manufacture method and application, specifically, the present invention relates to a kind of microbial fertilizer with nutrition, biological control and growth promoting effect and its manufacture method, and the different application mode of this microbial fertilizer and cultivation in different crops in the application. Background technique: [0002] Fertilizer is one of the most basic agricultural production materials. Humans first used some natural fertilizers, such as manure, and later developed compost, and then artificial fertilizers. The earliest artificial fertilizers were chemical synthetic fertilizers, referred to as chemical fertilizers. For more than 100 years, chemical fertilizers have played an important role in agricultural production. However, with the increase of chemical fertilizer application, the damage to the soil structure, the negative impact on the ecological env...

Claims

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Application Information

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IPC IPC(8): A01G7/00C05F11/08C12N1/00C12N1/20
Inventor 刘小秧
Owner SOIL & FERTILIZER INST CHINESE ACAD OF AGRI SCI
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