53results about How to "Strong inhibitory effect" patented technology

The invention belongs to the field of grain storage, and particularly relates to a cinnamon oil contained insect repelling mildew preventive for grain storage. The insect repelling mildew preventive is prepared from the following raw materials in parts by weight: 10-15 parts of cinnamon oil, 6-10 parts of garlic essential oil, 3-5 parts of clove essential oil, 0.5-2 parts of lemongrass essential oil, 1-3 parts of essential oil of zanthoxylum maxim, 2-5 parts of star anise oil and 50-65 parts of ethanol. The insect repelling mildew preventive is prepared from natural plant essential oil, is safe and environmentally friendly and is free of pollution, and each single raw material or the combination adopted by the insect repelling mildew preventive has uniquefungicidal and anti-bacterial effects and has an distinct effect of killing common pests in a grain storage granary in a fumigation and contact manner.

The invention relates to Bacillus subtilis and the application thereof in prevention and treatment of continuous cropping obstacle of greenhouse vegetable. The Bacillus subtilis is M-2 strain with the preservation number of CGMCC No. 2271. The M-2 strain is plant growth-promoting rhizobacteria separated from oceanic mangrove plant, which can restrain the growth of various harmful fungi such as fusarium wilt of cucumber, root rot of tomato, the root rot of soybean and the like, and can effectively prevent and treat the continuous cropping obstacle of the greenhouse vegetable and increase the quality and output of the greenhouse vegetable.

The invention discloses an application of bacteroides fragilis in resisting aquacultural pathogenic bacteria. The application of the bacteroides fragilis in resisting the aquacultural pathogenic bacteria comprises the application of the bacteroides fragilis in preparation of an antibacterial medicine for the aquacultural pathogenic bacteria, and medicinal compositions and feed additives which contain the bacteroides fragilis. The bacteroides fragilis comprises living bacteria of bacteroides fragilis, inactivated bacteroides fragilis, bacteroides fragilis lysates and / or bacteroides fragilis culture supernatant fluid. According to the application of the bacteroides fragilis in resisting the aquacultural pathogenic bacteria disclosed by the invention, experiments prove that the bacteroides fragilis has strong inhibiting effects on the aquacultural pathogenic bacteria, such as vibrio parahaemolyticus, vibro splendidus, vibrio harveyi, vibrio anguillarum or pseudomonas aeruginosa, does not have medicine resistance, and is safe and non-toxic. Meanwhile, the medicinal compositions and the feed additives which contain the bacteroides fragilis also have the same or similar effects.

The invention discloses bacillus amyloliquefaciens and application thereof in control of potato late blight, and belongs to the technical field of control of crop disease. The bacillus amyloliquefaciens strain is Bacillus amyloliquefaciens GZ5, and is registered and preserved in the China General Microbiological Culture Collection Center on November 21, 2019, and a preservation number is CGMCC No.18987. Fermentation liquor of the Bacillus amyloliquefaciens GZ5 can effectively inhibit growth of phytophthora infestans. The strain GZ5 is obtained from healthy tomato rhizosphere soil in Guangze County, Fujian Province, has good compatibility with environments, is non-toxic, does not have pathogenicity, is wide in antimicrobial spectrum, has antagonism towards multiple kinds of pathogens of alternaria solani, botrytis cinerea, gloeosporium eriobotryae, sclerotinia sclerotiorum, bacillus anthracis, pestalotiopsis on anoectochilus roxburghii, rhizoctonia solani, taxus wallichiana leaf blightpathogens, botryosphaeria rhodina and the like, and thus has good application and development prospects.

The invention provides a 4,6-dihydro-benzo [h] tetrazole [5,1-b] quinazoline-7,8-diketone and a synthetic method of the 4,6-dihydro-benzo [h] tetrazole [5,1-b] quinazoline-7,8-diketone. The method comprises the steps that 5-amino-1H-tetrazole, paraformaldehyde, 2-hydroxyl-1, 4- naphthoquinone and toluene sulfonic acid at a catalyst amount are evenly mixed, heated and stirred, the temperature of the mixture is controlled to be in the range of 120-130 DEG C, and the reaction time is 1-3 hours. The 4, 6-dihydro-benzo [h] tetrazole [5, 1-b] quinazoline-7, 8-diketone is a heterozygote of adjacent naphthoquinone and tetrazol [1, 5-a] pyrimidine and is high in antitumor activity. The synthetic method has the advantages that the environment-friendly effect is achieved, raw materials are easy to obtain, operations are simple, and the yield is high.

The invention discloses a bacillus licheniformis AM-53 used for controlling cunninghamia lanceolata anthracnose, and an application thereof. The bacillus licheniformis has a collection number of CCTCC No: M2012273. The bacillus licheniformis AM-53 provided by the invention has a high inhibiting effect against colletotrichum gloeosporioides, and has an important significance in cunninghamia lanceolata anthracnose controlling. The bacillus licheniformis AM-53 has an important effect in plant disease bio-control. The strain has simple culturing conditions, and is easy to store. Industrialized production of the strain is easy. The strain had good development and application prospect.

The invention discloses antibacterial mouthwash. The antibacterial mouthwash is prepared from the following components in parts by weight: 8 to 15 parts of ethanol, 0.2 to 0.5 part of sodium chloride,0.03 to 0.08 part of sodium fluoride, 1.5 to 3 parts of sodium dodecyl sulfate, 0.03 to 0.06 part of sodium monofluorophosphate, 2 to 5 parts of flos lonicerae, 1 to 3 parts of propolis, 1 to 3 partsof chamomile, 0.5 to 2 parts of thymol, 0.5 to 1 part of food sweetner, 0.3 to 0.8 part of food preservative and the balance of deionized water, wherein the total amount of the components is 100 weight parts. The mouthwash disclosed by the invention has stronger inhibiting effects on staphylococcus aureus and candida albicans; the clearance rate of oral bacteria reaches 20 percent or more; two oral diseases of decayed tooth and periodontal diseases can be effectively prevented; in addition, pure natural Chinese medicinal components such as the flos lonicerae and the chamomile are adopted to serve as antibacterial components, so that flora balance in an oral cavity is controlled while effective sterilization is realized, and the damage to teeth and oral mucosa is avoided.

The invention discloses sophora tonkinensis endophytic fungus TRXY-34-1. The sophora tonkinensis endophytic fungus TRXY-34-1 has the type name of Fusarium solani TRXY-34-1, an ITS sequence of the Fusarium solani TRXY-34-1 is as shown in SEQ ID NO. 1, and the Fusarium solani TRXY-34-1 is collected by the General Microorganisms Center of China Committee for Culture Collection of Microorganisms, which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on May 13, 2015 and has the collection number of CGMCC No. 10767. According to the sophora tonkinensis endophytic fungus TRXY-34-1, the endophytic fungus Fusarium solani TRXY-34-1 is obtained through being separated from roots of a medicinal plant, i.e., Sophora tonkinensis and carrying out screening for the first time and has a powerful inhibiting action on Fusarium solani, and thus a broad application prospect is brought to the field of biological control on panax notoginseng fungal diseases.

This invention provides a compound represented by formula (1) or a pharmaceutically acceptable salt thereof. Specifically, the present invention provides a compound represented by formula (1) or a pharmaceutically acceptable salt thereof [Therein, A is O, S, or N—R6; ring G is a 5-membered or 6-membered aromatic ring, etc., including 1-3 heteroatoms selected from O, S and N as constituent atoms; R1 and R2 are each independently a hydrogen atom, a halogen atom, or an optionally-substituted C1-6 alkyl carbonyl group, etc.; R3, R4 and R5 are each independently a hydrogen atom, a halogen atom, or an optionally-substituted C1-6 alkyl carbonyl group, etc.; and R6 is a hydrogen atom or an optionally-substituted C1-6 alkyl group, etc.].

The invention relates to a preparation method of growth-promoting synbiotic bacterium liquid and application. The preparation method comprises the steps of adding complex of synanthrin and astragalus polysaccharide into an MRS (Methicillin Resistant Staphylococcus) culture medium at a proper ratio, filtering and separating by adopting a microporous filtering membrane and collecting a filtrate; inoculating activated lactobacillus casei on the filtrate of the culture medium containing the complex of the synanthrin and the astragalus polysaccharide, and placing in an anaerobic incubator with temperature of 37 DEG C for standing culture, thus obtaining the synbiotic bacterium liquid containing the synanthrin, the astragalus polysaccharide and the activated lactobacillus casei; the synbiotic is added into complete feed and the effect of feeding and mixing simultaneously can be achieved; the synanthrin and the astragalus polysaccharide can provide nutrition for the lactobacillus casei in intestinal tracts and generate the acid environment to enhance multiplication of beneficial bacteria, and by complementary relation of the synanthrin, the astragalus polysaccharide and the lactobacillus casei, the inhibiting action on pathogenic microorganisms is strong; defense mechanisms of organisms can be resisted, the intestinal tracts of the organisms can be adjusted to maintain the healthy state; the immunocompetence is high, so that the quantity of pathogenic bacteria is reduced and the existence of pathogenic harmful bacteria and exogenous germs can be inhibited.

The invention belongs to the technical field of plant disease biocontrol, and particularly relates to a novel Burkholderia cenocepacia XJYC 2 and application in the prevention and control of litchi downy blight disease and litchi anthraenose. The novel Burkholderia cenocepacia is named as the Burkholderia cenocepacia XJYC 2 which is deposited in the Guangdong Microbial Culture Collection Center, referred to as GDMCC, the address is the Guangdong Institute of Microbiology, floor 5, building 59, courtyard No.100, Xianlie Middle Road, Guangzhou, the preservation number is GDMCC No: 60699, and thenovel Burkholderia cenocepacia is deposited on June 17, 2019. The invention further provides a biological preparation based on the novel Burkholderia cenocepacia. The novel Burkholderia cenocepacia and the biological preparation thereof have significant inhibitory effects on both the peronophythora litchii and litchi anthraenose, and have good biological control effect and broad application prospects.

The present invention relates to a medicine composition with the function of resisting inflammation. Said medicine composition is formed from (by weight portion) 1-6 portions of prunella spike, 1-5 portions of eucalyptus leaf and 1-4 portions of lonicera flower. Said invention also provides its preparation method and concrete steps. Said medicine composition can be made into injection or eye drops, and can be used for curing the diseases of pelvic inflammation, cervicitis, salpingitis, vulvitis, vaginitis, conjunctivitis, keratitis and trachoma, etc, and can obtain obvious therapeutic effect.

The present invention belongs to the field of pharmaceuticals and chemical industry, and discloses preparation of a quinoline-based derivative based on topoisomerase I inhibition, and uses of the quinoline-based derivative as an anti-inflammatory drug, wherein the structural formula of the quinoline-based derivative is represented by a formula (I), R1 is hydrogen, fluorine, chlorine, bromine, iodine, methoxy, methyl, trifluoromethyl, nitro and nitrile, R2 and R3 respectively are hydrogen, chlorine, bromine, iodine, methoxy, methyl, trifluoromethyl, nitro and nitrile, R4 is carboxyl, methyl, phenyl, cyano and hydrogen, n is 0, 1, 2 and 3, and X is CH or N. According to the present invention, the quinoline-based derivative can remarkably inhibit the activity of human DNA topoisomerase I, provides significant inhibition effects in inflammations, and has broad application space in the preparation of anti-inflammatory drugs. The formula (I) is defined in the specification.

The invention provides a method for constitutive expression of a target antibacterial peptide AgPlectasin in Pichia pastoris by use of a GAP promoter. According to the method, an AOX promoter in a vector pPICAgPlectasin is replaced by the GAP promoter to construct a constitutive expression vector pGAPAgpPICAgPlectasin and transform Pichia pastoris X-33; and the obtained recombinant yeast is subjected to fermentation culture and can secrete and generate AgPlectasin. The method provided by the invention realizes constitutive expression of the target antibacterial peptide AgPlectasin in Pichia pastoris for the first time, and after 120 hours of culture, the total protein concentration in the fermentation liquid reaches 532.4mg / L; the supernate of the fermentation liquid is subjected to G25desalination and SP ion-exchange chromatography, and a pure target product can be obtained with an output of 118.37mg / L; and the purity is 93.27% according to Genetools analysis. Bacteriostasis experiments indicate that AgPlectasin has a strong inhibition effect on the ATCC standard strain of staphylococcus aureus, and the MIC of AgPlectasin is 0.09-1.47muM. The AgPlectasin obtained by the method provided by the invention can be applied to the fields of antibacterial drugs, food additives, cosmetics, feed additives and the like, and has high application value and broad market prospect.

The present invention discloses a novel antimicrobial peptide and an application thereof in citrus preservation. A linear tetrapeptide which has a strong inhibition effect on rotten fungi after citrusharvesting is separated out from fermentation liquid of penicillum oxalicum SG-4 by steps of ethanol precipitation, concentration and freeze-drying, high performance liquid chromatography, etc. Aftermagnetic resonance imaging, high-resolution mass spectrometry and other spectrum analysis, the substance is found to be a propylene glycol monomethyl ether connected linear 4-peptide compound Thr-Thr-Val-Ser (propanediol tetrapeptide, PTP for short), parts of peptide bonds and alpha-amino groups show methylation, and structures and functions of the type of the compound are not reported internationally. Experiments show that the PTP has the strong inhibition effect on rotten fungi penicillium italicum after the citrus harvesting, antimicrobial functions also exist when PTP is at a low concentration of 1 [mu]g / mL, and the effect of the PTP is significantly better than commonly used citrus preservatives of bellkute, a prochloraz and imazalil compound, carbendazim, etc. in the market. The antibiotic is a potential citrus preservative and can be used on a large scale in industrial and agricultural production.

The invention discloses an N-naphthyl-alpha-amido aryl phosphonic acid, with a general molecular formula (I). The invention also discloses a preparation method and a use of the N-naphthyl-alpha-amido aryl phosphonic acid. The N-naphthyl-alpha-amido aryl phosphonic acid is prepared from 1-naphthylamine, aromatic aldehyde and phosphorous acid based on a mannich reaction at the presence of acid, wherein the molar ratio of the 1-naphthylamine, the aromatic aldehyde, the phosphorous acid and concentrated hydrochloric acid is 1:(1-1.4):(1-1.4):(0.9-1.1), reaction temperature is 100-150 DEG C, and reaction time is 1-3 hours. The N-naphthyl-alpha-amido aryl phosphonic acid disclosed by the invention has the advantages of simple synthesizing process and short reaction time, and the synthesized compound has a stronger inhibiting effect on phosphate minerals and can be used in a neutral or weakly alkaline condition.

Provided is a method for treatment of atopic dermatitis and infectious dermatitis with biological spa therapy. The method seeks to cure or alleviate symptoms of atopic dermatitis by bathing in a bathwater containing, as dominant bacteria, not less than 105 Bacillus bacteria per 1 mL of the bathwater.

The invention discloses a sophora tonkinensis endophytic bacterium B21. The sophora tonkinensis endophytic bacterium B21 is classified and named as Burkholderia sp. B21, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10806. According to the sophora tonkinensis endophytic bacterium B21 provided by the invention, a strain of endophytic bacterium Burkholderia sp. B21 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Burkholderia sp. B21 has strong inhibiting effect on panax notoginseng colletotrichum gloeosporioides, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention belongs to the biological pharmacy technical field, in particular relates to streptoseomycin extracted from marine actinomycete streptomyces seoulensis liquid fermentation as well as preparation method and application thereof. Streptoseomycin is obtained by separation, purification and fermentation on streptomyces seoulensis from ocean. Streptoseomycin has strong inhibition function on anaerobe and has no toxicity on cell, thus streptoseomycin can be taken as lead compound of antibiotic production and can be applied in preparation of antibiotic medicine.

The invention belongs to the field of cosmetics, and relates to anti-acne essence and a preparation method thereof, the anti-acne essence includes the raw materials of four components of A, B, C and D, the B component includes propylene glycol, water, peony root extract, honeysuckle extract and garcinia mangostana peel extract in formula I, and the B component includes water, butylene glycol and Spiraea Ulmaria extract in formula II. The anti-acne essence is prepared form effective acne removing ingredients according to a pure Chinese herbal formula, and is free of any added hormones and prohibited ingredients. The product is natural, mild, safe and effective in acne removing effects, and the preparation method is simple and controllable in process and low in cost, and is suitable for wideapplication.

Belonging to the technical field of genetic engineering antibodies, the invention specifically discloses a human vascular endothelial growth factor receptor (VEGFR2 or KDR) targeted antibody JZB00 and a fusion antibody JZA02 of an interferon alpha (IFN alpha) mutant, a preparation method and application thereof. The invention also discloses amino acid sequences of the heavy chain and light chain immunoglobulin molecules of JZA02. The invention also provides a construction method of JZA02 heavy chain and light chain gene, CHO cells are transfected, monoclone is selected by means of limiting dilution method, and then eukaryotic cell secretory expression and affinity chromatography purification are carried out to obtain the fusion antobody. The fusion antibody provided by the invention can specifically bound to VEGFR2, and can inhibit tumor angiogenesis, also a coupling mutation interferon part can play a direct part of tumor killing and immunoregulation, and no toxic or side effect is generated, thus better inhibiting tumor growth. The fusion antibody can inhibit human umbilical vein endothelial cells (HUVEC) in vitro and the proliferation of part of tumor cells, and the inhibition degree is higher than that of a fusion antibody JZA01 of wild type IFN alpha and JZB00.

The invention relates to a preparation method of dendrobium aurantiacum powder. The preparation method comprises the following steps of (1) performing cleaning treatment: removing leaves of fresh dendrobium aurantiacum, adding the dendrobium aurantiacum without leaves in cleaning liquid, performing soaking and cleaning, performing fishing out, and removing surface moisture for standby application;(2) performing drying: drying the dehydrated dendrobium aurantiacum until the water content is 60-70%, and performing cutting into slices; (3) performing freezing treatment: freezing the dendrobium aurantiacum slices; (4) performing differential pressure puffing treatment: performing differential pressure puffing on the frozen dendrobium aurantiacum slices, to prepare the puffed dendrobium aurantiacum slices; (5) performing low-temperature reflow extraction and concentration: adding an organic solvent to the puffed dendrobium aurantiacum slices, and performing low-temperature reflow extraction and concentration to prepare primary concentrate; (6) performing concentration: sending primary concentrate into a spherical vacuum concentrating tank, and performing concentration to prepare secondary concentrate; (7) performing drying and crushing: drying a secondary concentrated solution, and performing crushing, to prepare crude dendrobium aurantiacum powder; and (8) preparing components. According to the method provided by the invention, the bioactive components of the dendrobium aurantiacum are low in loss rate, high in dissolving and leaching efficiency and high in biology absorptionand utilization rate.

The invention discloses an externally used traditional Chinese medicine composition capable of avoiding allergy and relieving itching. The externally used traditional Chinese medicine composition is prepared from, by weight, 40 to 70 parts of raspberry, 20 to 40 parts of fructus ligustrum lucidum, 10 to 40 parts of haematococcus pluvialis, 30 to 50 parts of frutas galangae, and 10 to 20 parts of oriental cherry flower. It is confirmed by experiments that the externally used traditional Chinese medicine composition possesses extremely excellent inhibition effects on hyaluronidase, so that the externally used traditional Chinese medicine composition possesses excellent allergy prevention effect. The externally used traditional Chinese medicine composition is also capable of relieving itching preferably. Skin care preparations prepared from the externally used traditional Chinese medicine composition are capable of avoiding generation of bloodshot skin preferably. Raw materials are easily available; a preparation method is simple, green, and safe; the externally used traditional Chinese medicine composition can be accepted by allergy sufferers; and application prospect and market prospect are promising.

The invention discloses pantala flavescens larva intestinal tract endophytic fungus Curvularia crepinii and an application thereof. The pantala flavescens larva intestinal tract endophytic fungus Curvularia crepinii QTYC-1 fermentation liquor can significantly inhibit the growth of radicles of barnyard grass and amaranthus retroflexus; the maximum inhibition rate is up to 95.0% and 90.1% respectively; and the damage rate of barnyard grass seedlings sprayed with the fermentation liquor reaches 71.1%. Ethyl acetate extract of the fermentation liquor has very good inhibition effect on radicles of barnyard grass and amaranthus retroflexus; at a concentration of 100 [mu]g / mL, the inhibition activities of the crude extract on barnyard grass and amaranthus retroflexus are 56.8% and 71.2% respectively; at the above concentration, the extract has very good safety for common crops; the inhibition rate is less than 35%, which is comparable to the activity of a positive control of cycloheximide; a very good candidate drug is provided for the development of new herbicides and antibiotic drugs.

The invention discloses a power grid phase detection system. The system comprises a voltage detection link, a lowpass filtering link, a notch link, and further comprises an amplitude detecting link, and the lowpass filtering link and the notch link are embedded in a phase-locked loop; the invention further includes a phase detection method. According to the system and the method, through the embedded lowpass filtering link and notch link, low-frequency, medium-high-frequency harmonic components of power grid voltage are suppressed and the process of controller tuning is simplified without considering the influence of phase delay.

The invention provides an oxazole histone deacetylase inhibitor and a preparation method thereof. The preparation method is characterized by comprising the following steps: dissolving an oxazole compound 1A by using an NH2OK solution, adding hydroxylamine, stirring at room temperature, detecting a reaction endpoint by using TLC, removing a solvent under reduced pressure, adding a hydrochloric acidsolution for acidification, extracting by using dichloromethane, and drying by distillation to obtain a crude product; the oxazole compound provided by the invention has a very strong inhibition effect on histone deacetylase and cancer cells, and compared with histone deacetylase inhibitors on the market, the oxazole compound provided by the invention has the advantages that the activity of the oxazole compound on histone deacetylase is remarkably improved, has a better effect on solid tumors such as lung cancer, and can overcome the problems of low activity, poor curative effect on solid tumors and the like of the existing histone deacetylase inhibitor.

The invention belongs to the field of antitumor drug preparation, in particular to a 1-methyl-7H-indene[1, 2-b]quinolinetrifluoromesylate-7-(4-dimethylamino)benzyl alkene derivant used as a DNA topoisomerase inhibitor and a preparation method thereof. The preparation method comprises the following steps of: (1) salification reaction: reacting 7H-indene [1, 2-b]quinoline with methyl trifluoromethanesulfonate in the molar ratio of 1 to (2-3) under the protection of nitrogen gas at room temperature with stirring to obtain 7H-indene[1, 2-b]quinolinetrifluoromesylate; (2) coupling reaction: reacting the obtain 7H-indene[1, 2-b]quinolinetrifluoromesylate and 4-dimethylaminobenzaldehyde in the molar ratio of 1.0:(1.4-1.6) in glacial acetic acid in refluxing to prepare the 1-methyl-7H-indene[1, 2-b]quinolinetrifluoromesylate-7-(4-dimethylamino)benzyl alkene derivant. The prepared compound has strong inhibition action on leukemia cells and human laryngeal squamous carcinoma cells; moreover, the preparation method has the advantages of simple process and good yield reaching about 65%.

The invention discloses an aminodisuccinic or iminodisuccinic succinate application. The application refers to that aminodisuccinic or iminodisuccinic succinate is used as an olivine inhibitor in mineral flotation. The invention further discloses a using method of the olivine inhibitor in ilmenite; after floatation desulphurization of the ilmenite, acid is added so that pH of a flotation system iscontrolled to be 2-5; and before a flotation collector is added, the olivine inhibitor is added. The olivine inhibitor is higher in chelating capability, and stronger in adsorption to olivine; the olivine inhibitor is applied to the refractory ilmenite flotation technology, and the selective inhibition on the olivine can be enhanced; and the grade and recovery rate of titanium concentrate are improved. Meanwhile, the olivine inhibitor is liable to degrade, and soil and water pollution is not caused; and the olivine inhibitor is an environmental friendly green chemical.

The invention provides an application of a ferroptosis inducer RSL3 in preparation of a medicine for inhibiting glioblastoma, and also provides a medicine for combined application of the ferroptosis inducer RSL3 and temozolomide. The invention provides a more effective treatment scheme for glioblastoma, and also provides a solution capable of remarkably inhibiting glioblastoma under the condition of temozolomide resistance.

The invention discloses crassostrea gigas DM9-domain-containing protein CgDM9CP-2. The amino acid sequence of the protein is shown as SEQ ID NO.1. The preparation method includes the sequential stepsthat PCR amplification is performed on crassostrea gigas CgDM9CP-2 gene coding sequence fragments with specific primers P1 and P2, wherein the DNA sequence of the primer P1 is shown as SEQ ID NO.2, and the DNA sequence of the primer P2 is shown as SEQ ID NO.3; a PCR amplification product and a pET-30 a carrier are subjected to Nde1 and Xho1 digestion and then connected through ligase to be transformed, and sequencing authentication of a recombinant is performed; the recombinant is transferred into an escherichia coli Transetta (DE3) expression strain for induced culture, then, purification andrenaturation are performed, and the recombinant protein with the amino acid sequence shown in the sequence table SEQ ID NO.1 is obtained. The rassostrea gigas DM9-domain-containing protein CgDM9CP-2can be applied to preparation of pichia pastoris inhibiting medicine.