Novel antimicrobial peptide and application thereof in citrus preservation

Abstract

The present invention discloses a novel antimicrobial peptide and an application thereof in citrus preservation. A linear tetrapeptide which has a strong inhibition effect on rotten fungi after citrusharvesting is separated out from fermentation liquid of penicillum oxalicum SG-4 by steps of ethanol precipitation, concentration and freeze-drying, high performance liquid chromatography, etc. Aftermagnetic resonance imaging, high-resolution mass spectrometry and other spectrum analysis, the substance is found to be a propylene glycol monomethyl ether connected linear 4-peptide compound Thr-Thr-Val-Ser (propanediol tetrapeptide, PTP for short), parts of peptide bonds and alpha-amino groups show methylation, and structures and functions of the type of the compound are not reported internationally. Experiments show that the PTP has the strong inhibition effect on rotten fungi penicillium italicum after the citrus harvesting, antimicrobial functions also exist when PTP is at a low concentration of 1 [mu]g/mL, and the effect of the PTP is significantly better than commonly used citrus preservatives of bellkute, a prochloraz and imazalil compound, carbendazim, etc. in the market. The antibiotic is a potential citrus preservative and can be used on a large scale in industrial and agricultural production.

Description

technical field [0001] The present invention relates to the isolation and identification of an antimicrobial peptide PTP that has an inhibitory effect on Penicillium citrus, which can be mainly applied to the inhibition of the citrus postharvest rot-causing bacterium Penicillium citrus and the postharvest preservation of citrus. Background technique [0002] Citrus is one of the pillar industries of the rural economy in southern my country, but in recent years, the phenomenon of seasonal oversupply is very prominent, and its storage period is very short. Corruption and quality deterioration occur, causing huge economic losses. Although chemical fungicides such as Baket and Prochloraz have a certain effect on the preservation of citrus, the quality of the treated fruit deteriorates, the sugar content is reduced, and it has toxic side effects on the human body, and the residual time is long and pollutes the environment. Many countries have banned the use of chemical fungicides...

AI Technical Summary

Problems solved by technology

[0002] Citrus is one of the pillar industries of the rural economy in southern my country, but in recent years, the phenomenon of seasonal oversupply is very prominent, and its storage period is very short. Corruption and quality deterioration, resulting in huge economic losses

Although chemical fungicides such as Baket and Prochloraz have a

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