Application of sophora tonkinensis endophytic fungus TRXY-34-1 in prevention and treatment of panax notoginseng root rot

A technology of endophytic fungi and Fusarium solani, applied in the biological field, can solve problems such as threats to health, pollution, and production reduction of Panax notoginseng, and achieve strong inhibitory effects and broad application prospects

Active Publication Date: 2016-04-13
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional authentic production area of ​​Sanqi in Guangxi, due to the low altitude and high temperature and humidity weather conditions, this disease often occurs at the same time, resulting in a large amount of production reduction or even failure of Sanqi, and brought h

Method used

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  • Application of sophora tonkinensis endophytic fungus TRXY-34-1 in prevention and treatment of panax notoginseng root rot
  • Application of sophora tonkinensis endophytic fungus TRXY-34-1 in prevention and treatment of panax notoginseng root rot
  • Application of sophora tonkinensis endophytic fungus TRXY-34-1 in prevention and treatment of panax notoginseng root rot

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Experimental program
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Effect test

Embodiment 1

[0024] Isolation, screening and identification of strains

[0025] 1. Isolation of strains

[0026] Test material: wild Vietnamese pagoda tree collected from the limestone area of ​​Tiandeng County, Guangxi.

[0027] Tested strains: provided by Institute of Plant Pathology, Guangxi University.

[0028] Medium: 1000ml potato dextrose agar medium (PDA medium), PDA medium contains 300g of potatoes, 20g of glucose, 20g of agar and 0.1g of chloramphenicol, and the pH of the medium is 6.0±0.2.

[0029] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (availa...

Embodiment 2

[0076] The Inhibitory Effect of the Metabolites of the Strains on the Root Rot of Panax notoginseng

[0077] 1. Fermentation culture of bacteria and extraction of fermentation products

[0078] (1) Inoculate the endophytic fungus TRXY-34-1 of Sophora vietnamese on a flat plate of potato dextrose agar medium (PDA plate), culture it at 28°C for 20 days, cut the obtained culture material into pieces and transfer to In the Erlenmeyer flask of 2 liters of aseptic solid culture medium (containing the dextrose of 400g potato, 20g and 20g sucrose), place 28 ℃ of conditions to ferment for 60 days;

[0079] (2) After the fermentation is completed, soak the fermented product with 2 times the amount of methanol of the fermented product and sonicate it for 40 minutes, then filter it with gauze, and get the filtrate;

[0080] (3) Repeat step (2) twice, combine the two filtrates and concentrate under reduced pressure to form an extract, and obtain the methanol crude extract of the fermentat...

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Abstract

The invention discloses sophora tonkinensis endophytic fungus TRXY-34-1. The sophora tonkinensis endophytic fungus TRXY-34-1 has the type name of Fusarium solani TRXY-34-1, an ITS sequence of the Fusarium solani TRXY-34-1 is as shown in SEQ ID NO. 1, and the Fusarium solani TRXY-34-1 is collected by the General Microorganisms Center of China Committee for Culture Collection of Microorganisms, which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on May 13, 2015 and has the collection number of CGMCC No. 10767. According to the sophora tonkinensis endophytic fungus TRXY-34-1, the endophytic fungus Fusarium solani TRXY-34-1 is obtained through being separated from roots of a medicinal plant, i.e., Sophora tonkinensis and carrying out screening for the first time and has a powerful inhibiting action on Fusarium solani, and thus a broad application prospect is brought to the field of biological control on panax notoginseng fungal diseases.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to the application of a Vietnamese Sophora japonica endophyte TRXY-34-1 in the prevention and treatment of Panax notoginseng root rot. Background technique [0002] The prevention and control of plant diseases in modern agriculture is overly dependent on the use of chemical pesticides. The application of a large number of chemical pesticides not only has long-term damage to the ecological environment, but also causes the quality of agricultural products to decline, pesticide residues exceed the standard, pathogenic bacteria are resistant to drugs, and are harmful to humans and animals. Many questions. It is of great significance to find safer and more effective methods of disease control, and the use of biological methods to prevent and control plant diseases can effectively solve the above problems. [0003] Panax notoginseng F.H.chen, also known as Tianqi, Jinbuhuan, etc., ha...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02A01N63/04A01P3/00C12R1/77
CPCA01N63/30C12P1/02C12N1/145C12R2001/77
Inventor 黄荣韶姚裕群蓝芳李良波
Owner GUANGXI UNIV
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