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Bacillus amyloliquefaciens and application thereof in control of potato late blight

A technology for potato late blight and amylolytic spores, applied in the fields of application, bacteria, and fungicides, can solve problems such as unsatisfactory chemical control effects and environmental pollution, and achieve good development and application prospects, broad antibacterial spectrum, and strong inhibitory effect Effect

Active Publication Date: 2020-06-30
INST OF PLANT PROTECTION FAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of Bacillus amyloliquefaciens and its application in the prevention and treatment of potato late blight in view of the severe occurrence of potato late blight at present, the area is expanding year by year, the chemical control effect is not ideal, and the problem of environmental pollution is brought at the same time

Method used

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  • Bacillus amyloliquefaciens and application thereof in control of potato late blight
  • Bacillus amyloliquefaciens and application thereof in control of potato late blight
  • Bacillus amyloliquefaciens and application thereof in control of potato late blight

Examples

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Effect test

Embodiment 1

[0024] Example 1 Isolation and identification of antagonistic bacteria

[0025] l. Soil sample collection and strain isolation

[0026] Gently shovel off the topsoil around the rhizosphere of healthy tomato plants, use a soil sampler to take a soil sample at a depth of 5-10 cm, pour it into a sterilized ziplock bag, make real-time records, and take it back to the laboratory for isolation . Randomly weigh 10 g of each soil and place it in a 250 mL Erlenmeyer flask filled with 90 mL of sterile water and a small amount of glass beads. After the bottle mouth is fixed with a sealing film, it is shaken on a shaker at 200 r / min for 30 min, and after standing for 5 min, , draw 1 ml of the soil sample suspension with a pipette gun, add it into a test tube filled with 9 ml of sterile water and mix well, that is 10 -2 The same method was used to prepare 10 times soil sample dilution solution in turn. -3 、10 -4 、10 -5 、10 -6 times soil sample dilution. Take 100 ul of the prepared...

Embodiment 2

[0035] Example two: Inhibitory effect of bacterial strain GZ5 live body on the mycelial growth of potato late blight pathogen

[0036] Take the spare rye medium and LB medium, melt them in the microwave, mix them in equal proportions and pour them onto plates. Inoculate the mycelium block of P. infestans (D=7 mm) in the center of the mixed culture dish of rye medium and LB in equal proportions, and dip the GZ5 on the plate with an inoculation loop at a distance of 2.5 cm from the opposite sides of the mycelium block. Bacterial liquid was streaked, and only the mycelial block of P. infestans of the same size was connected as a control, repeated 3 times, placed in a constant temperature incubator at 20 ℃ for cultivation, and the antibacterial rate was counted. The result is as figure 2 As shown, when the control P. infestans covered the petri dish, the growth of P. amyloliquefaciens in the test group showed the phenomenon of evading Bacillus amyloliquefaciens GZ5, and the bact...

Embodiment 3

[0037] Embodiment three: the inhibitory action of bacterial strain GZ5 fermented liquid to the mycelia growth of P. infestans

[0038] Add 2 mL of the fermentation broth of strain GZ5 to 20 mL of melted rye medium cooled to about 45 ℃, mix and invert the plate to make an antibacterial plate of the fermentation liquid of strain GZ5, and inoculate potato latex with a diameter of 7 mm in the center of the plate. Phytophthora mycelium block. Add 2 mL of LB liquid medium to 20 mL of melted rye medium cooled to about 45 °C, mix and pour the plate, and inoculate the mycelial block of P. infestans with a diameter of 7 mm in the center of the plate as a control. Repeat 3 times, culture in a 20°C incubator, measure the growth diameter of the colony by the cross method, and calculate the inhibition rate. The result is as image 3 The results showed that the fermentation broth of strain GZ5 could significantly inhibit the mycelial growth of P. infestans. After 14 days of cultivation, th...

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Abstract

The invention discloses bacillus amyloliquefaciens and application thereof in control of potato late blight, and belongs to the technical field of control of crop disease. The bacillus amyloliquefaciens strain is Bacillus amyloliquefaciens GZ5, and is registered and preserved in the China General Microbiological Culture Collection Center on November 21, 2019, and a preservation number is CGMCC No.18987. Fermentation liquor of the Bacillus amyloliquefaciens GZ5 can effectively inhibit growth of phytophthora infestans. The strain GZ5 is obtained from healthy tomato rhizosphere soil in Guangze County, Fujian Province, has good compatibility with environments, is non-toxic, does not have pathogenicity, is wide in antimicrobial spectrum, has antagonism towards multiple kinds of pathogens of alternaria solani, botrytis cinerea, gloeosporium eriobotryae, sclerotinia sclerotiorum, bacillus anthracis, pestalotiopsis on anoectochilus roxburghii, rhizoctonia solani, taxus wallichiana leaf blightpathogens, botryosphaeria rhodina and the like, and thus has good application and development prospects.

Description

technical field [0001] The invention relates to a biocontrol bacterial strain for preventing and treating potato late blight and an application thereof, belonging to the technical field of crop disease prevention and control. Background technique [0002] by Phytophthora infestans ( Phytophthora infestans (Mont) de Bary) is a devastating disease in potato production. It occurs and is prevalent in all major potato producing areas in the world, and it seriously threatens potato production. The damage it causes and the difficulty of control And its impact on society has surpassed rice blast and wheat stripe rust, and is regarded as the largest crop disease in the world. Late blight is common and serious in our country, causing an economic loss of about 8 billion yuan each year due to late blight, which has become the first major obstacle to restricting potato production and industrialization in my country. [0003] So far, the control of potato late blight mainly relies on th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C05G3/60C12R1/07
CPCC12N1/20C05G3/00C12R2001/07C12N1/205
Inventor 李本金王荣波刘裴清林金龙陈庆河翁启勇
Owner INST OF PLANT PROTECTION FAAS
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