Bacillus amyloliquefaciens and application thereof in control of potato late blight

A technology for potato late blight and amylolytic spores, applied in the fields of application, bacteria, and fungicides, can solve problems such as unsatisfactory chemical control effects and environmental pollution, and achieve good development and application prospects, broad antibacterial spectrum, and strong inhibitory effect Effect

Active Publication Date: 2020-06-30
INST OF PLANT PROTECTION FAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain of Bacillus amyloliquefaciens and its application in the prevention and treatment of potato late blight in view of the severe

Method used

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  • Bacillus amyloliquefaciens and application thereof in control of potato late blight
  • Bacillus amyloliquefaciens and application thereof in control of potato late blight
  • Bacillus amyloliquefaciens and application thereof in control of potato late blight

Examples

Experimental program
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Example Embodiment

[0024] Example 1 Isolation and identification of antagonistic bacteria

[0025] l. Soil sample collection and strain separation

[0026] Gently shovel the topsoil around the rhizosphere of healthy tomato plants, use a soil sampler to take a soil sample with a depth of 5-10 cm, pour it into a sterilized spare ziplock bag, make real-time records, and take it back to the laboratory for separation . Weigh 10g of each soil randomly and place it in a 250-mL Erlenmeyer flask containing 90 mL of sterile water and a small amount of glass beads. After fixing the sealing film at the mouth of the bottle, place it on a 200 r / min shaker and shake for 30 minutes. After standing for 5 minutes , Use a pipette to draw 1 ml of soil sample suspension, add it to a test tube containing 9 ml of sterile water, and mix it well, that is, 10 -2 Times the soil sample dilution, using the same method to prepare 10 -3 , 10 -4 , 10 -5 , 10 -6 Times soil sample dilution. Take 100 ul of the prepared soil sample...

Example Embodiment

[0035] Example 2: Inhibition of strain GZ5 in vivo on the growth of hyphae of potato late blight pathogen

[0036] Take the spare rye medium and LB medium, melt them in a microwave oven, mix and pour the plate in equal proportions. Inoculate the Phytophthora infestans mycelium block (D=7 mm) in the center of a mixed petri dish of rye medium and LB in equal proportions. At a distance of 2.5 cm from the opposite sides of the mycelium block, dip the GZ5 on the plate with an inoculation loop Streak the bacterial solution, and take only the same size of the potato infestation fungus hyphae block as a control, repeat 3 times, place it in a 20 ℃ constant temperature incubator, and count the inhibition rate. The result is figure 2 As shown, when the control Phytophthora infestans grows on the petri dishes, the growth of Phytophthora infestans in the test group shows a phenomenon that circumvents Bacillus amyloliquefaciens GZ5, and the inhibition rate is 67.14% after 14 days of culture, ...

Example Embodiment

[0037] Example 3: Inhibition of fermentation broth of strain GZ5 on the growth of Phytophthora infestans hyphae

[0038] Add 2 mL of the fermentation broth of strain GZ5 to 20 mL of melted rye culture medium cooled to about 45 ℃ and mix the plate to make a bacteriostatic plate of the fermentation broth of strain GZ5. Inoculate potato late with a diameter of 7 mm in the center of the plate. Phytophthora hyphae block. Add 2 mL of LB liquid medium to 20 mL of melted rye medium that has been cooled to about 45 ℃ and mix the plate, and also inoculate a 7 mm diameter potato infestation hyphae block in the center of the plate as a control. Repeat 3 times, culture in a 20°C incubator, measure the colony growth diameter by the cross method, and calculate the inhibition rate. The result is image 3 As shown, the fermentation broth of strain GZ5 can significantly inhibit the growth of the hyphae of Phytophthora infestans. The colony growth diameter is 21 mm after 14 days of culture, and th...

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Abstract

The invention discloses bacillus amyloliquefaciens and application thereof in control of potato late blight, and belongs to the technical field of control of crop disease. The bacillus amyloliquefaciens strain is Bacillus amyloliquefaciens GZ5, and is registered and preserved in the China General Microbiological Culture Collection Center on November 21, 2019, and a preservation number is CGMCC No.18987. Fermentation liquor of the Bacillus amyloliquefaciens GZ5 can effectively inhibit growth of phytophthora infestans. The strain GZ5 is obtained from healthy tomato rhizosphere soil in Guangze County, Fujian Province, has good compatibility with environments, is non-toxic, does not have pathogenicity, is wide in antimicrobial spectrum, has antagonism towards multiple kinds of pathogens of alternaria solani, botrytis cinerea, gloeosporium eriobotryae, sclerotinia sclerotiorum, bacillus anthracis, pestalotiopsis on anoectochilus roxburghii, rhizoctonia solani, taxus wallichiana leaf blightpathogens, botryosphaeria rhodina and the like, and thus has good application and development prospects.

Description

technical field [0001] The invention relates to a biocontrol bacterial strain for preventing and treating potato late blight and an application thereof, belonging to the technical field of crop disease prevention and control. Background technique [0002] by Phytophthora infestans ( Phytophthora infestans (Mont) de Bary) is a devastating disease in potato production. It occurs and is prevalent in all major potato producing areas in the world, and it seriously threatens potato production. The damage it causes and the difficulty of control And its impact on society has surpassed rice blast and wheat stripe rust, and is regarded as the largest crop disease in the world. Late blight is common and serious in our country, causing an economic loss of about 8 billion yuan each year due to late blight, which has become the first major obstacle to restricting potato production and industrialization in my country. [0003] So far, the control of potato late blight mainly relies on th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C05G3/60C12R1/07
CPCC12N1/20C05G3/00C12R2001/07C12N1/205
Inventor 李本金王荣波刘裴清林金龙陈庆河翁启勇
Owner INST OF PLANT PROTECTION FAAS
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