85 results about "Colletotrichum coccodes" patented technology

The invention provides a streptomyces, which is named as Streptomyces sp. And is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65917. The invention also provides a fermentation broth of the streptomyces, and an ethanol extract and a crude extract of the fermentation broth. The novel streptomyces, the crude extract thereof and the like have broad-spectrum antibacterial activity, have a good antagonistic effect on various pathogenic bacteria such as banana long leaf spot germs and banana colletotrichum gloeosporioides, can enable hyphae of the banana colletotrichum gloeosporioides to shrink, deform and break, inhibit spore germination of the banana colletotrichum gloeosporioides, and have a good anti-bacterial effect on the banana colletotrichum gloeosporioides. According to the present invention, with the application of the bacterial strain, the colletotrichum gloeosporioides spores are shrunk and broken, the growth of the colletotrichum gloeosporioides is inhibited, the peroxidase activity, the polyperoxidase activity, the CAT activity and the like in the banana peel can be improved, and the bacterial strain is the potential biological agent for the prevention and control of the colletotrichum gloeosporioides diseases, and has wide development space and good development and application prospects.

The invention provides application of a pseudomonas parabarium YC15 strain in efficient prevention and treatment of wheat stem rot and various pathogenic bacteria. The invention provides a new application of the pseudomonas parabarium YC15 strain in prevention and control of plant diseases, and research shows that the YC15 strain has broad-spectrum bacteriostatic activity, has remarkable inhibitory activity on various plant pathogenic bacteria, can inhibit pathogenic fungi such as wheat stem rot, sesame root rot and colletotrichum gloeosporioides, and has a wide application prospect in prevention and control of plant diseases. And the compound has a relatively good inhibition effect on pathogenic bacteria, namely sesames pseudomonas solanacearum. The YC15 strain is applied to prevention and control of wheat stem rot, and pot experiments show that after the YC15 strain is adopted for treatment, plant lesions can be reduced, the inhibition effect of pathogenic bacteria on plants can be broken, the pathogenic bacteria can be effectively inhibited, plant growth can be promoted, and the wheat stem rot can be efficiently prevented and controlled. The invention provides new application of the YC15 strain, and the YC15 strain can be used for preventing and treating various plant protobacteria and diseases thereof, and has good development and application potential as a biocontrol bacterium.

The invention provides an isothermal amplification primer probe set for detecting Colletotrichum fucicola of Carya illinoensis, a rapid detection kit, a method for detecting Colletotrichum fucicola and application of the isothermal amplification primer probe set and the rapid detection kit, and belongs to the technical field of biological detection. Aiming at the limitation problems that the current apocarya colletotrichum gloeosporioides is difficult to identify, excessively depends on expensive instruments and technicians and the like, the apocarya colletotrichum gloeosporioides LFD-RPA detection technology research is developed, the apocarya colletotrichum gloeosporioides can be simply, conveniently and efficiently detected, the apocarya colletotrichum gloeosporioides LFD-RPA has no cross reaction with 22 representative strains of the colletotrichum gloeosporioides, the specificity is high, the detection time is the lowest detection limit is 10 pg of genome DNA, and the sensitivity is high. The method is suitable for port quarantine, scientific research institutions, agriculture and forestry management, farmer households and other departments to rapidly detect the diseases in real time on site.

The invention discloses bacillus for preventing and treating anthracnose of camellia oleifera and application of the bacillus. The preservation number of the bacillus CH-1-7 provided by the invention is GDMCC (Glucose Digital Microorganism Center) No. 66219; the bacillus amyloliquefaciens has the capability of generating living-promoting substances, can generate organic phosphorus and siderophores, has the nitrogen fixation capability, can inhibit the growth of various pathogenic bacteria, especially colletotrichum gloeosporioides, can be used for preventing and treating camellia oleifera anthracnose, and has important application value in the field of plant protection.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The invention discloses low-temperature-resistant rice endophytic streptomyces with biological prevention and control effects on common crop diseases and application of the low-temperature-resistant rice endophytic streptomyces, relates to the field of microorganisms, and aims to solve the problems that an existing rice blast biocontrol strain cannot play a stable effect under a low-temperature condition and is poor in rice blast control effect. The low-temperature-resistant rice endophytic streptomycete is alfalfa streptomycete F05 and is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 17, 2025, and the preservation number is CGMCC No.33539. The streptomyces medicaginis F05 disclosed by the invention can stably play a biological prevention and control role at a low temperature of 5-15 DEG C. The compound has broad-spectrum antibacterial performance, is used for inhibiting corn stalk rot bacteria, green bean colletotrichum, rice bakania, botrytis cinerea and rhizoctonia solani, and can also be used for decomposing starch and cellulose.

The invention belongs to the field of comprehensive utilization of palm leaves, and particularly relates to a natural preservative containing palm leaf extract as well as a preparation method and application of the natural preservative. According to the natural preservative disclosed by the invention, sunflower discs, betel nut leaves, mint leaves, oil-tea cakes and barley are added into lactobacillus plantarum to prepare fermentation liquor, the fermentation liquor is sterilized, and then palm leaf extract and purslane extract are added, so that the antibacterial rate on mango colletotrichum gloeosporioides, litchi fruit colletotrichum gloeosporioides and strawberry colletotrichum gloeosporioides can be remarkably increased; the preservative can effectively prevent fruits from decaying and deterioration, can effectively prolong the storage life of fruits when being used for preservation and fresh keeping of fruits, has small toxic and side effects, and greatly improves the safety.

The invention relates to the field of microorganisms, in particular to a broad-spectrum antagonistic bacterium bacillus velezensis SJD33 and application thereof. The preservation number of the bacillus velezensis SJD33 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.29456. The bacillus velezensis SJD33 provided by the invention has the advantages that the preservation number is CGMCC No.29456; the strain has a remarkable inhibition effect on pear fruit rot pathogens (physalospora piricola, penicillium, alternaria alternata, athelia fruit rot pathogens, botrytis cinerea and colletotrichum gloeosporioides) and other fruit pathogenic bacteria (apple core pathogens, brown rot pathogens and pomegranate dry rot pathogens). The SJD33 can obviously reduce the natural rotting rate of the pear fruits; the SJD33 can be rapidly colonized in fruit wounds; the SJD33 does not influence the fruit quality and can delay the reduction of the fruit hardness and the L-ascorbic acid; the SJD33 secondary metabolite is stable in activity and has great application potential. The strain provides a new strain resource for a fruit disease biocontrol resource library.

The invention discloses application of a CsNOMT gene and sakuranetin in prevention and treatment of tea tree anthracnose. It is found for the first time that methyltransferase encoded by the tea tree-derived CsNOMT gene can efficiently catalyze naringenin to be converted into sakuranetin, the endogenous phytoalexin level of a tea tree is remarkably improved, and then the disease resistance of the tea tree-derived CsNOMT gene to Colletotrichum carolinae is enhanced. The silence of the CsNOMT gene can reduce the sakuranetin content by 30-40%, resulting in the increase of the scab area; and by overexpressing the gene, the sakuranetin content is increased by 30%, and the scab area is remarkably reduced. In addition, the growth of colletotrichum can be effectively inhibited by exogenously spraying 0.05-0.3 mM of sakuranetin, and the optimal concentration is 0.1-0.3 mM. The invention provides a new strategy for green prevention and control of tea tree anthracnose, and has the characteristics of high efficiency, safety and environmental friendliness.

The invention relates to the field of agricultural biotechnology and plant protection, and particularly provides application of 2-heptanone to preparation of a preparation for preventing and treating plant diseases. The bactericidal composition has a strong bacteriostatic effect on litchi colletotrichum gloeosporioides, strawberry colletotrichum gloeosporioides, mango leaf blight, pawpaw colletotrichum gloeosporioides, wheat gibberellic disease, mango colletotrichum gloeosporioides, tomato fusarium wilt, banana fusarium wilt, banana colletotrichum gloeosporioides and corn root rot. The fumigant developed on the basis of 2-heptanone and used for preventing and treating phytopathogen is expected to be used for preventing and treating compound diseases caused by various pathogenic bacteria, or comprehensively preventing and controlling different crop diseases in a crop rotation system, and has important development value.

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.

The present application provides a kind of streptomyces, named Streptomyces sp., is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No:65917.The present application also provides fermentation broth of the streptomyces and ethanol extract and crude extract of fermentation broth thereof.The new species of streptomyces and its crude extract and the like of the present application have broad-spectrum antibacterial activity, have good antagonism to many pathogenic bacteria such as banana long-shaped spot fungus and banana anthracnose fungus, and can make banana anthracnose fungus hyphae wrinkle, deform, break, inhibit banana anthracnose fungus spore germination, make banana anthracnose fungus spore germination wrinkle, break, inhibit banana anthracnose fungus growth, and can also improve peroxidase, polyperoxidase, CAT activity and the like in banana peel, is a potential biological agent for preventing and treating anthracnose disease, stem rot disease and the like, has broad development space, and has good development and application prospect.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

The invention discloses a novel methoxyimino acetate derivative containing a chloroacetamide structure as well as a preparation method and application of the novel methoxyimino acetate derivative. According to the invention, a methoxyimino acetate skeleton is used as a leading structure, and different pharmacophores are introduced on the basis of the leading structure, so that a series of novel methoxyimino acetate skeleton derivatives containing a chloroacetamide structure are synthesized. In-vitro tests of the compounds show that the compounds have excellent inhibition effects on plant pathogenic fungi such as fusarium graminearum, eggplant verticillium wilt, mango anthracnose pathogen, alternaria solani, sclerotinia sclerotiorum, muskmelon fusarium wilt pathogen and the like; the methoxyl acrylate derivative pesticide with high activity and novel action mechanism is expected to be developed.

This invention relates to the field of crop genetics and breeding, and discloses the sweet sorghum disease resistance gene CPK4, its encoded protein, and its applications. The nucleotide sequence of the sweet sorghum disease resistance gene CPK4 is shown in SEQ ID NO.1, or has more than 90% homology with the sequence shown in SEQ ID NO.1 and encodes a protein with a nucleotide sequence that promotes the release of reactive oxygen species (ROS) in plants. By utilizing the CPK4 gene cloned from sweet sorghum, and through the specific interaction between its encoded protein and the NADPH oxidase SbRbohD protein, the ROS signaling pathway induced by pathogens is specifically activated, strengthening the defense response of sweet sorghum against anthracnose. This achieves a targeted enhancement of sweet sorghum's resistance to anthracnose. Furthermore, this gene is specifically induced by the molecular patterns of anthracnose and pathogens, and its disease resistance mechanism differs from existing NLR-mediated immune pathways, enriching the types of sweet sorghum disease resistance gene resources.

The application discloses a kind of compounds with carbonyl coupling rigid fragment and its preparation method and bactericidal purposes.The compound has the structure shown in general formula I.A rigid carbonyl skeleton is constructed by connecting amino-containing 1,2,4-triazole through amide bond, and maleic acid structure and bioactive biological amine fragment are introduced, which are integrated in the same molecular skeleton by carbonyl coupling method.The compound synthesis route is short, the reaction condition is mild, and the yield is high.The biological activity test results show that the compound has broad-spectrum and significant inhibitory activity on rice sheath blight fungus, tomato botrytis cinerea, rice blast fungus, grape anthracnose fungus, oilseed rape sclerotinia sclerotiorum and many other plant pathogenic fungi.The compound has multi-target synergistic mechanism and low risk of drug resistance.

The application discloses a pear anthracnose resistance gene MYC2- like and application, and belongs to the field of plant genetic engineering. MYC2‑like The nucleotide sequence of the gene is shown as SEQ ID NO:1, the protein sequence encoded by the gene is shown as SEQ ID NO:2, the gene is significantly up-regulated in response to MeJA and anthracnose fungus induction; the gene is located in the plant cell nucleus and has a conserved bHLH domain; a MYC2‑like MYC2‑like The constructed overexpression vector is used for transforming Solanum lycopersicum callus, so that the resistance of the callus to Colletotrichum gloeosporioides is increased, and the application provides an important gene resource for cultivating pear anthracnose resistance varieties.

The invention belongs to the technical field of pesticides, and discloses a pesticide composition for tea tree diseases and a preparation method and application thereof. The pesticide composition is prepared from alpha-ethylidene-phenylacetaldehyde. Tests prove that the alpha-ethylidene-phenylacetaldehyde has an obvious inhibiting effect on the growth of tea anthracnose pathogen hyphae and can be used for preventing and treating tea anthracnose. According to the invention, alpha, alpha-ethylidene-phenylacetaldehyde is used as a synthetic spice for food, it is found for the first time that alpha, alpha-ethylidene-phenylacetaldehyde can be used as a potential disease control agent for control of tea tree diseases, has the characteristics of high efficiency, safety and no pollution, and effectively overcomes the problem of chemical pesticide residues.

The present invention relates to a method for preparing a fermentation liquid of Streptomyces hygroscopicus and its application. The method for preparing a fermentation liquid of Streptomyces hygroscopicus comprises the following steps: inoculating a fermentation medium with a strain named Streptomyces hygroscopicus JN3-4, and fermenting and culturing, wherein the deposit number of Streptomyces hygroscopicus JN3-4 is CGMCC No. 27649. The fermentation liquid prepared by the above method can be used for biological control of pathogenic bacteria such as Aggregative Small Hole Shell Fungus, Red Bean Anthracnose Bacteria, Red Pine Blight Bacteria, Corn Big Spot Bacteria, Tobacco Target Spot Bacteria, Rice Bakanae Bacteria, Melon Vine Cut Bacteria, Pepper Anthracnose Bacteria, Pear Boy's Disk Fruit Rot Bacteria, Cucumber Sclerotinia Bacteria, Blueberry Canker Bacteria, Larch Blight Bacteria, etc., and has the advantages of good biological control effect, wide antibacterial spectrum, wide application range, etc.

The application provides a cycloalkyl-substituted triazolopyridine amide compound and a preparation method and application thereof, and belongs to the technical field of pesticides. The cycloalkyl-substituted triazolopyridine amide compound provided by the application introduces an unsubstituted or substituted cyclohexyl group or an unsubstituted or substituted cyclopentyl group on a triazole ring, has excellent inhibitory effect on pepper Phytophthora capsici Leonian, and has an EC 50 as low as 0.3 micromole / L; and the cycloalkyl-substituted triazolopyridine amide compound and derivatives thereof also have broad-spectrum inhibitory activity on rice Helminthosporium leaf spot fungus, wheat Helminthosporium leaf spot fungus, brassica sclerotinia, wheat Gibberella zeae, wheat take-all fungus, tomato botrytis cinerea, potato late blight fungus, pepper Phytophthora capsici Leonian, tomato early blight fungus, rice bacterial wilt fungus, potato dry rot fungus, cucumber anthracnose fungus and rice rice blast fungus, and have a good application prospect in the preparation of anti-plant pathogenic fungus drugs.

The invention relates to the technical field of bioengineering, in particular to a coffee reverse osmosis protein gene and application thereof. The nucleotide sequence of the coffee reverse osmosis protein gene is as shown in SEQ ID NO. 1. The amino acid sequence of the coffee reverse osmosis protein coded by the coffee reverse osmosis protein gene is as shown in SEQ ID NO. 2. The coffee reverse osmosis protein has antibacterial biological activity, can be used for preparing antibacterial products, is applied to prevention and control of crop diseases, and can inhibit growth of Colletotrichum gloeosporioides, sugarcane red rot bacteria and tea leaf blight bacteria.

The present invention relates to the technical field of microbial control, and discloses a Bacillus velezensis nanomaterial composite microbial agent, a preparation method thereof and an application. The microbial agent of the present invention is prepared by the following steps: preparing a bacterial suspension of Bacillus velezensis; after mixing sodium alginate with the bacterial suspension, adding nano-carbon and stirring to obtain a mixed solution; adding the mixed solution into a stirred calcium chloride solution, and reacting to obtain a Bacillus velezensis microcapsule microbial agent. The microbial agent of the present invention has an antibacterial rate of >56% against Citrus brown spot pathogen, Citrus anthracnose pathogen, Citrus greasy spot and yellow spot pathogen, Banana wilt pathogen, Banana long-shaped spot pathogen; the control effect on Huanglongbing reaches 57.84%. After being irradiated by ultraviolet rays, the antibacterial ability of the microbial agent of the present invention only decreases by 4.32%, and it can play a biological control role for a long time. The growth amount of citrus seedlings treated with the microbial agent of the present invention can be increased by 220.39%, and the number of newly grown leaves can be increased by 244.67%, having a good plant growth promotion effect.

The application discloses a strain of Brevibacillus sp. HNCS-1, relates to the technical field of tea tree planting, and has a preservation number of CCTCC NO: M2022713. The strain is classified and named as Brevibacillus sp., and is preserved in the China Center for Type Culture Collection on May 25, 2022, and is located at Wuhan University, Wuhan, China, and has a postal code of 430072. The strain shows significant inhibitory effects on common pathogenic bacteria in tea gardens, such as tea anthracnose bacteria, tea leaf penicillium, tea fusarium, tea white star bacteria, tea round brown spot disease, and tea wheel spot disease, is environment-friendly, and is non-toxic and non-harmful.

The invention provides a heterocyclic ring-containing nicotinamide compound, a synthesis method and application of the heterocyclic ring-containing nicotinamide compound in prevention and treatment of plant fungal diseases, and relates to the technical field of pesticides. The heterocyclic ring-containing nicotinamide compound is synthesized by using easily available raw materials and a simple, practical and low-cost synthesis method. The compound has a synergistic effect on rhizoctonia solani, magnaporthe oryzae, mango anthracnose pathogen, rubber anthracnose pathogen, litchi anthracnose pathogen, banana anthracnose pathogen, coffee anthracnose pathogen, fusarium graminearum, wheat powdery mildew pathogen, valsa mali, botrytis cinerea, sclerotinia sclerotiorum, sigatoka musae, banana scab pathogen, apple ring spot pathogen, botrytis cinerea, botrytis cinerea, botrytis cinerea, botrytis cinerea and botrytis cinerea. The compound has an excellent inhibiting effect on plant fungal diseases caused by fungi such as tobacco alternaria alternata, cotton rhizoctonia solani, exserohilum turcicum and hevea brasiliensis, is novel in structure, and has huge potential for creation of novel pesticides.

This invention discloses a PEG-mediated genetic transformation method for *A. anthracnose* protoplasts. Through the preparation, transformation, and transformation of *A. anthracnose* protoplasts, and the screening of transformants, the hygromycin B resistance gene fragment was successfully transferred into the *A. anthracnose* genome, achieving successful genetic transformation of *A. anthracnose* protoplasts. More importantly, this invention establishes a new enzymatic digestion system and improves the culture mechanism in protoplast preparation. During the culture and screening of transformants, a more suitable resistance concentration is used, balancing the stringency of screening with the survival of transformants. This improves transformation efficiency, shortens the overall transformation process, and establishes a more efficient and convenient genetic transformation system for *A. anthracnose* protoplasts.

The invention belongs to the technical field of plant disease and insect pest prevention and control, and particularly relates to application of nerol in prevention and control of rubber anthracnose. It is found for the first time that nerol with low concentration (0.5 [mu] L / mL) can effectively inhibit colletotrichum gloeosporioides, phytophthora capsici and colletotrichum gloeosporioides, and especially the inhibition rate of nerol to colletotrichum gloeosporioides and colletotrichum gloeosporioides can reach 100%. Besides, nerol also has obvious inhibitory activity on rubber anthrax bacteria inoculated on in-vitro leaves, and can obviously reduce the area of scabs, and the inhibition rate reaches 98.4%. The results show that the nerol has a good prevention and treatment effect on the rubber anthracnose, the nerol belongs to a plant-sourced volatile compound, and few residues are left when the nerol is used for preventing and treating the rubber tree anthracnose, so that the nerol can be used as a potential green pesticide for preventing and treating the rubber anthracnose, and a new way is provided for preventing and treating the rubber anthracnose.

The invention discloses a fungus strain 41233 from Western sand and application of the fungus strain 41233, the taxonomic name of the fungus strain 41233 is Plectosphaerella sp., the preservation number of the fungus strain 41233 is GDMCC No.67505, and the preservation date of the fungus strain 41233 is December 19, 2025. The invention further discloses application of the fungus strain 41233 and a crude extract of the fungus strain 41233 in preparation of pathogenic bacterium inhibition products. The fungus strain 41233 sourced from Xiaxa has inhibitory activity on mango colletotrichum gloeosporioides and / or phytophthora capsici, and a crude extract of the fungus strain 41233 sourced from Xiaxa has inhibitory activity on various pathogenic bacteria such as colletotrichum gloeosporioides, phytophthora and fruit rot pathogens, and can be used for preparing biocontrol inoculants or further mining active natural products. And a new green prevention and control means is provided for plant disease prevention and control.

The present invention provides a biocontrol yeast strain and an application thereof. The yeast strain is named Meyerozyma caribbica G31887. The Meyerozyma caribbica G31887 was deposited in the General Microbiology Center of China Culture Collection Administration on November 3, 2021, with the address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number being CGMCC No. 23714. The Meyerozyma caribbica G31887 can grow normally under the conditions of a temperature of 10°C-50°C, a pH of 3-11, and the addition of 0.1%-10% NaCl to the culture medium. The yeast fermentation liquid of the present invention has an inhibitory effect on watermelon wilt pathogen, longan scorch rot pathogen, pear anthracnose pathogen, pear ring spot pathogen, pear black spot pathogen, loquat anthracnose pathogen and grape gray mold pathogen, has certain temperature, acid, alkali and salt tolerance, and has a good control effect on grape gray mold, providing a strain screening source for biological control of grape gray mold.

The invention provides application of banana root exudates or an ethanol extract of the banana root exudates in preparation of 10-hydroxydecanoic acid. The new streptomyces is used for inducing the change of banana root exudates, the key root exudates 10-hydroxydecanoic acid can be obtained through separation, gibberellic disease and the like can be effectively antagonized, but no inhibition effect is achieved on banana fusarium oxysporum fourth physiological race and the like; however, the 10-hydroxydecanoic acid can effectively inhibit the fourth physiological race of the banana wilt pathogen in the banana rhizosphere soil, effectively prevent and treat the banana wilt caused by the fourth physiological race of the banana wilt pathogen, improve the abundance of Sphingomonas bacteria, Bacillus bacteria, Pseudolaabrys bacteria and the like in the banana rhizosphere soil, and improve the yield of the banana wilt disease caused by the fourth physiological race of the banana wilt pathogen and the banana wilt disease caused by the fourth physiological race of the banana wilt pathogen. The invention provides a new method and idea for preventing and controlling fusarium wilt and preventing and controlling wheat gibberellic disease, strawberry colletotrichum gloeosporioides, banana colletotrichum gloeosporioides, litchi colletotrichum gloeosporioides, mango colletotrichum gloeosporioides, grape botrytis

The invention relates to the technical field of fruit trees, in particular to a pear anthracnose resistance evaluation method. The method comprises the following steps: (1) rejuvenating and culturing colletotrichum gloeosporioides; (2) inoculating and culturing pear fruit germplasm resource fruits by using the colletotrichum gloeosporioides obtained in the step (1); (3) measuring the diameter of the scab at the 9th day of inoculation, and calculating the average value of the diameter of the scab; and (4) according to the average value of the scab diameters of the 9th day obtained in the step (3), dividing the resistance of the pear germplasm resources to the anthracnose germs into five grades by a systematic clustering analysis method, and taking the five grades as a criterion for judging the resistance of the pear fruits to the anthracnose germplasm germplasm resources to the anthracnose germplasm germplasm resources. The pear anthracnose resistance evaluation method is not limited by the external environment, is short in evaluation period, low in cost and simple to operate, and can greatly improve the screening efficiency of pear fruit resistance germplasm resources.