Pantala flavescens larva intestinal tract endophytic fungus Curvularia crepinii and application thereof

A technology of endophytic fungi and larvae, applied in the direction of application, fungi, biocides, etc., can solve the problems of human production and health hazards, agricultural product rot, economic loss, etc., achieve good inhibition, simple cultivation conditions, and eliminate production The effect of craft

Inactive Publication Date: 2015-04-22
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phytopathogenic fungi can cause rot, necrosis and wilting of agricultural products, causing serious economic losses, and may a

Method used

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  • Pantala flavescens larva intestinal tract endophytic fungus Curvularia crepinii and application thereof
  • Pantala flavescens larva intestinal tract endophytic fungus Curvularia crepinii and application thereof
  • Pantala flavescens larva intestinal tract endophytic fungus Curvularia crepinii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The isolation and screening of embodiment 1 bacterial strain

[0027] The tested yellow dragon larvae were collected from the river near the campus of Zhejiang Normal University (29°00’17.37”N, 119°29’54.84”E). Disinfect the surface of healthy yellow dragonfly larvae with 75% alcohol for 2 minutes, rinse with sterile water for 3 times, put the intestinal tract obtained after dissection into a sterile mortar for grinding, and use sterile water to gradiently dilute the grinding solution to 10 -1 , 10 -2 , 10 -3 0.2 mL of each gradient dilution was spread on MEA medium (recipe: 20 g of malt extract, 20 g of sucrose, 20 g of agar, 1 g of peptone, 1 L of distilled water, pH 7.0) plate, and cultured in an incubator at 28°C. After the colony grew out, a small amount of hyphae was picked from the edge of the colony, and transferred to the MEA medium plate again, thus obtaining a pure culture of bacterial strain, named QTYC-1, and preserved in the preservation medium (same as ...

Embodiment 2

[0028] Example 2 Identification of endophytic fungus Curvularia crepinii QTYC-1 in the intestinal tract of yellow dragonfly larvae

[0029] 1. Morphological identification

[0030] Its ecological characteristics are as follows: the morphological characteristics of the strain and the preliminary identification are based on the "Identification Handbook of Semi-Knowledgeable Fungi". Such as figure 1 As shown, in MEA (formulation: 20g of malt extract, 20g of sucrose, 20g of agar, 1g of peptone, 1L of distilled water, pH 7.0, sterilized at 120°C for 20min) on a medium plate at a constant temperature of 28°C for 10 days, the diameter can reach 70~ 80mm, the hyphae are light green at first, and then gradually turn dark green, short fluffy, compact, and the colonies are nearly round and flat, with irregular edges. Mycelium is colorless or light brown, septate, multi-branched, 2.0-4.1 μm wide. Conidiophores solitary or in clusters, produced from hyphae terminal and central cells, le...

Embodiment 3

[0034] Example 3 Preparation of endophytic fungus Curvularia crepinii QTYC-1 fermentation broth and fermentation broth extract in the intestinal tract of yellow dragonfly larvae

[0035] Inoculate the fresh fungus Curvularia crepinii QTYC-1 mycelium block into a 250mL conical flask containing ME medium (the formula is: 20g of malt extract, 20g of sucrose, 1g of peptone, 1L of distilled water, pH 7.0, sterilized at 120°C for 20min ), cultivated for 2 to 3 days as the seed liquid; then inoculated the seed liquid into a 1000mL Erlenmeyer flask containing ME medium, and carried out expanded fermentation at 180r / min and 28°C to obtain the intestinal fungus Curvularia Liquid culture of crepinii QTYC-1. During the cultivation period, 50 mL of the fermentation liquid of the intestinal fungus Curvularia crepinii QTYC-1 from the larvae of the yellow dragonfly was taken under aseptic conditions every other day, filtered through two layers of gauze, and the mycelium was separated to obtai...

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Abstract

The invention discloses pantala flavescens larva intestinal tract endophytic fungus Curvularia crepinii and an application thereof. The pantala flavescens larva intestinal tract endophytic fungus Curvularia crepinii QTYC-1 fermentation liquor can significantly inhibit the growth of radicles of barnyard grass and amaranthus retroflexus; the maximum inhibition rate is up to 95.0% and 90.1% respectively; and the damage rate of barnyard grass seedlings sprayed with the fermentation liquor reaches 71.1%. Ethyl acetate extract of the fermentation liquor has very good inhibition effect on radicles of barnyard grass and amaranthus retroflexus; at a concentration of 100 [mu]g/mL, the inhibition activities of the crude extract on barnyard grass and amaranthus retroflexus are 56.8% and 71.2% respectively; at the above concentration, the extract has very good safety for common crops; the inhibition rate is less than 35%, which is comparable to the activity of a positive control of cycloheximide; a very good candidate drug is provided for the development of new herbicides and antibiotic drugs.

Description

technical field [0001] The invention relates to the technical field of using microorganisms to prevent and control farmland weeds and agricultural fungicides, in particular to a fermented liquid and extracts of the intestinal endophytic fungus Curvularia crepinii QTYC-1 used in the control of farmland weeds and agricultural fungicides. in the application. Background technique [0002] Barnyardgrass Echinochloa crusgalli is one of the top ten malignant weeds in the world, and it is the most important weed damage in rice fields, wheat fields and corn fields. Barnyardgrass is widely distributed throughout the country. It often grows as the dominant grass species in moist farmland, wasteland, roadsides, ditches, shallow ditches, ponds and swamps. It is harmful to crops such as rice, corn, beans, potatoes, cotton, cereals, and vegetables. In recent years, it has become the number one malignant weed in rice-producing areas, seriously affecting rice yield and quality. Amaranthus...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P13/02A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 张应烙邵明伟余文华
Owner ZHEJIANG NORMAL UNIVERSITY
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