Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof

A gram-positive, leech vibrio technology, applied in applications, bacteria, disinfectants, etc., to ensure high quality, safety effects, and significant application effects

Inactive Publication Date: 2010-02-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some microecological preparations still have many defects in the prevention and treatment of aquatic animal diseases, and Bdellovibrio bacteriophage has unique biological characteristics of lysing bacte

Method used

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  • Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof
  • Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof
  • Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1

[0048] (1) Separation and purification of Bdellovibrio BDS02

[0049] The host Aeromonas hydrophila (purchased from Guangdong Institute of Microbiology Culture Collection, number GIM1.172) was inoculated into 100mL sterilized LB (Luria-Bertani) liquid medium (10g peptone, 5g yeast extract) , Distilled water 1000mL, pH 7.2) in a Erlenmeyer flask, placed in a constant temperature shaker with a rotation speed of 200rpm and a temperature of 28°C for 16h to make it in the logarithmic growth phase. The culture solution was centrifuged at 6000 rpm for 20 minutes at a temperature of 4°C, the supernatant was discarded, and the precipitated bacteria were used 1mL DNB (dilute nutrient broth) liquid medium (nutrient broth 0.8g, casein acid hydrolysate 0.5 g, 0.1g yeast extract, dissolved in 1000mL distilled water, pH 7.2~7.6) after resuspension (concentration is about 3×10 10 cfu / mL) placed in a refrigerator at 4°C for later use.

[0050] Pre-treat the collected mud samples,...

Example Embodiment

[0062] Example 2

[0063] (1) Preparation of microbial preparations to eliminate Gram-positive pathogenic bacteria in aquatic products

[0064] The preparation process is the same as step (2) of Example 1, except that physiological saline is added to resuspend the Bdellovibrio bacteria. The concentration of Bdellovibrio BDS01 and BDS02 are 10 respectively. 10 pfu / mL, mixing Bdellovibrio BDS01 and Bdellovibrio BDS02 in a ratio of 1:1 to obtain a microbial preparation.

[0065] (2) Use microbial preparations to eliminate Gram-positive pathogenic bacteria before eating aquatic products

[0066] ①Prepare gram-positive pathogenic bacteria liquid contained in aquatic products

[0067] Mycobacterium tuberculosis (Mycobacteriμm tuberculosis ATCC607, purchased from China Collection of Common Microorganisms), use liquid medium (take the tender coconut water and filter it through a cotton gauze pad, and then filter it through Whatmann No. 1 filter paper, each 80mL tender coconut juice filtrate Ad...

Example Embodiment

[0072] Example 3

[0073] The microbial preparation containing only Bdellovibrio BDS02 is used to eliminate Gram-positive pathogenic bacteria during the transportation of aquatic products. The specific process is as follows:

[0074] The specifications of the test container, the test water, and the tilapia, vannamei, river clams, and hairy crabs used in the test were the same as in Example 1. Put 4 intact grids in each aquarium, put tilapia, vannamei prawns, river clams and hairy crabs into each grid, and put it flat in the aquarium immediately after closing the lid of the grid , Drain the excess water in the tank so that the water level just covers the grid. Before the experiment, it was disinfected by sodium dichloroisocyanurate, and the water temperature was 26°C. Continuous aeration with water during the experiment. Three aquariums were used as a control group. Only the gram-positive pathogenic bacteria liquid prepared in step (3) of Example 1 was added to the aquatic produc...

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Abstract

The invention discloses a bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and an application thereof. The invention separates to obtain bdellovibrio bacteriovorus BDS02 the length of flagellum of which is at least 1.8mu m. The bdellovibrio bacteriovorus with flagellum on the end is cambered single cell the size of which is 0.65*0.2 mu m when observed by an electron microscope. The two layer plating method is used for cultivating the bdellovibrio bacteriovorus bacterial strain at the temperature of 28 DEG C for four days to form transparent round negativecolony the diameter of which is 1-2mm. Before the microorganism preparation prepared by the bdellovibrio bacteriovorus BDS02 is eaten by aquatic products, common Gram-positive pathogenic bacteria comprising Mycobacterium tuberculosis, star-shaped nocardia asteroids, rhodococcus erythropolis, streptococcus faecalis, staphylococcus epidermidis, clostridium perfringens and corynebacterium glutamicum in the transportation process and cultivation process can be eliminated, which improves the safe factor of raw aquatic products for consumers, protects the health of the consumers and provides guarantee for greenly processing and producing the aquatic products.

Description

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Claims

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Application Information

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Owner SOUTH CHINA UNIV OF TECH
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