2results about How to "Good therapeutic effect" patented technology

The application is suitable for the field of biological medicine technology, and provides a preparation method and application of CAR-T cells loaded with biological calcified nanomaterials, which comprises the following steps: step one, mixing black phosphorus quantum dots, calcium peroxide and lipid components, and purifying by ultrasonic emulsification and ultrafiltration centrifugation to obtain BCLT with mitochondria-targeting molecule triphenylphosphine modified on the surface; step two, co-incubating CAR-T cells with membrane dye after staining, and adding a disulfide bond-containing reduction-responsive linker arm DSPE-S-S-NHS; and step three, co-incubating BCLT with CAR-T cells, so that the BCLT is connected to the surface of the CAR-T cells through a chemical bond to obtain CAR-T cells loaded with biological calcified nanomaterials. The application significantly improves the infiltration capacity and killing effect of the CAR-T cells on solid tumors, can relieve the problem of tumor heterogeneity, and has excellent biological safety and synergistic anti-tumor effect.

The invention belongs to the technical field of biology, and discloses application of isoliquiritigenin in preparation of a product for preventing and / or treating fish infected with a siniperca chuatsi and frog iridovirus (MRV), the EC50 of the isoliquiritigenin is 3.08 mu M, and the isoliquiritigenin has a remarkable in-vivo and in-vitro anti-MRV effect, so that the treatment administration effect is the best, the premixing administration is the second time, and the effect of preventing and / or treating the fish infected with the siniperca chuatsi and frog iridovirus is achieved. The result shows that the isoliquiritigenin possibly has a direct damage effect on MRV virus particles and inhibits MRV virus proliferation; besides, excessive inflammation and cell apoptosis caused by MRV infection can cause fish tissue damage and can promote virus proliferation and escape, and isoliquiritigenin can relieve tissue damage caused by MRV virus, inhibit virus proliferation and play a comprehensive antiviral role through anti-inflammatory and anti-apoptosis effects; meanwhile, the invention also provides a medicine containing isoliquiritigenin, a fish feed and a fish feed additive.